Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-inflammatory activity of Scutellaria Baicalensis root extract in lipopolysaccharide-induced RAW 264.7 cells

LPS로 유도된 RAW264.7세포주에서 황금뿌리 물추출물의 항염증활성

  • Lee, Ye Eun (Department of Efficacy Study, Institute of Jinan red ginseng) ;
  • Park, Hong Jin (Department of Efficacy Study, Institute of Jinan red ginseng) ;
  • Park, Chung-berm (Department of Efficacy Study, Institute of Jinan red ginseng) ;
  • Hwang, Seung-mi (Department of Efficacy Study, Institute of Jinan red ginseng)
  • Received : 2020.12.23
  • Accepted : 2021.02.28
  • Published : 2021.04.30
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Abstract

Scutellaria baicalensis has been used as a traditional medicine for diarrhea, dysentery, hypertension, hemorrhaging, insomnia, inflammation, and respiratory infections. This study examined the anti-inflammatory effect of Scutellaria baicalensis water extract (SWE) in lipopolysaccharide (LPS)-induced RAW 264.7 cells. To evaluate the anti-inflammatory effect of SWE, RAW 264.7 macrophages were stimulated with LPS to induce the production of inflammation-related factors, which were measured by western blotting. In RAW 264.7 cells, SWE inhibited the production of nitric oxide (NO) without causing cell toxicity. SWE also reduced the expression of inducible NO synthase and cyclooxygenase-2 protein, as well as the production of pro-inflammatory cytokines (such as tumor necrosis factor-α). The phosphorylation levels of the mitogen-activated protein kinase family members, such as JNK and p38, were also reduced by SWE. Thus, SWE could be used as a potential anti-inflammatory agent.

Scutellaria baicalensis water extract (SWE)는 지질 다당류 LPS로 유도된 RAW 264.7 세포에서 NO 및 전 염증성 사이토카인인 TNF-α의 생성을 세포 독성을 유발하지 않고 유의하게 억제하였다. 또한, SWE는 iNOS 및 COX-2의 단백질발현을 농도의존적으로 감소시켰으며, ERK, JNK, p38과 같은 MAPKs 계열의 인산화 발현 수준을 조사한 결과 JNK와 p38의 발현 수준을 감소시켰다. 이는 SWE가 p38 인산화를 억제함으로써 iNOS, COX, 그리고 TNF-α와 같은 전 염증성 사이토카인의 발현을 감소시키며 결론적으로 NO의 생성을 억제시킨다는 결과를 도출할 수 있었다. 본 연구는 항염증 효능 검증뿐 아니라 염증대사기전의 주요인자를 탐색함으로써 황금의 기능성 소재로써의 가능성을 시사한다.

Keywords

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