The Korean Journal of Physiology and Pharmacology

  • 제25권3호
  • /
  • Pages.251-258
  • /
  • 2021
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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Flos magnoliae constituent fargesin has an anti-allergic effect via ORAI1 channel inhibition

  • Hong, Phan Thi Lam (Department of Physiology, Dongguk University College of Medicine) ;
  • Kim, Hyun Jong (Channelopathy Research Center (CRC), Dongguk University College of Medicine) ;
  • Kim, Woo Kyung (Channelopathy Research Center (CRC), Dongguk University College of Medicine) ;
  • Nam, Joo Hyun (Department of Physiology, Dongguk University College of Medicine)
  • 투고 : 2021.02.25
  • 심사 : 2021.03.09
  • 발행 : 2021.05.01
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초록

Flos magnoliae (FM), the dry flower buds of Magnolia officinalis or its related species, is a traditional herbal medicine commonly used in Asia for symptomatic relief of and treating allergic rhinitis, headache, and sinusitis. Although several studies have reported the effects of FM on store-operated calcium entry (SOCE) via the ORAI1 channel, which is essential during intracellular calcium signaling cascade generation for T cell activation and mast cell degranulation, the effects of its isolated constituents on SOCE remain unidentified. Therefore, we investigated which of the five major constituents of 30% ethanoic FM (vanillic acid, tiliroside, eudesmin, magnolin, and fargesin) inhibit SOCE and their physiological effects on immune cells. The conventional whole-cell patch clamp results showed that fargesin, magnolin, and eudesmin significantly inhibited SOCE and thus human primary CD4+ T lymphocyte proliferation, as well as allergen-induced histamine release in mast cells. Among them, fargesin demonstrated the most potent inhibitory effects not only on ORAI1 (IC50 = 12.46 ± 1.300 μM) but also on T-cell proliferation (by 87.74% ± 1.835%) and mast cell degranulation (by 20.11% ± 5.366%) at 100 μM. Our findings suggest that fargesin can be a promising candidate for the development of therapeutic drugs to treat allergic diseases.

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참고문헌

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