The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Protective Effect of Arecae Semen and Coptidis Rhizoma in a Chronic Reflux Esophagitis Rat Model

만성 역류성 식도염 모델에서 빈랑(檳榔)와 황련(黃連) 복합물의 보호 효과

  • Lee, Se Hui (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Jin A (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ji Hye (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Roh, Seong-Soo (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이세희 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이지혜 (한국한의학연구원 한약자원연구센터) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2021.02.16
  • Accepted : 2021.04.05
  • Published : 2021.03.30
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Abstract

Objective: Chronic reflux esophagitis (CRE), characterized by esophageal mucosa ulcer, is caused by continuous backflow of gastric acid and consequent inflammation due to unstable gastroesophageal sphincter. The aim of the present study was to clarify the effect of an Arecae Semen and Coptidis Rhizoma mixture (AC-mix) on CRE. Methods: CRE was surgically induced in SD rats with three experimental groups used: normal; CRE control; and CRE treatment (200 mg/kg AC-mix). Blood and esophageal tissue were collected after two weeks of drug administration. The anti-oxidant activity of the AC-mix was measured by total polyphenol and total flavonoid contents as well as by radical scavenging activity with protein levels evaluated using western blotting. Results: CRE damage to the esophageal mucosa was significantly reduced in the AC-mix group as compared with the controls, and administration of the AC-mix was seen to inhibit NF-κBp65 activity. Consequently, the inactivation of NF-κBp65 significantly inhibited inflammatory mediators such as COX-2 and iNOS. Moreover, the anti-oxidant enzyme HO-1 significantly increased through activation of the Nrf2-Keap1 pathway. Matrix metalloproteinase-2 (MMP-2), which can break down collagen from the basement membrane and extracellular matrix, was decreased following AC-mix treatment, and elevated levels of MMP-2 were regulated by its tissue inhibitor. Conclusions: These results show that AC-mix can alleviate esophageal mucosa ulcer though inhibition of the NF-κBp65 inflammatory pathway and enhancement of the anti-oxidant Nrf2-Keap1 pathway.

Keywords

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