Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Integrated analysis of transcriptomic and proteomic analyses reveals different metabolic patterns in the livers of Tibetan and Yorkshire pigs

  • Duan, Mengqi (College of Animal Science, Tibet Agriculture and Animal Husbandry University) ;
  • Wang, Zhenmei (College of Animal Science, Tibet Agriculture and Animal Husbandry University) ;
  • Guo, Xinying (College of Animal Science, Tibet Agriculture and Animal Husbandry University) ;
  • Wang, Kejun (College of Animal Sciences and Veterinary Medicine, Henan Agricultural University) ;
  • Liu, Siyuan (College of Animal Science, Tibet Agriculture and Animal Husbandry University) ;
  • Zhang, Bo (National Engineering Laboratory for Animal Breeding/Beijing Key Laboratory for Animal Genetic Improvement, China Agricultural University) ;
  • Shang, Peng (College of Animal Science, Tibet Agriculture and Animal Husbandry University)
  • Received : 2020.05.16
  • Accepted : 2020.09.13
  • Published : 2021.05.01
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Abstract

Objective: Tibetan pigs, predominantly originating from the Tibetan Plateau, have been subjected to long-term natural selection in an extreme environment. To characterize the metabolic adaptations to hypoxic conditions, transcriptomic and proteomic expression patterns in the livers of Tibetan and Yorkshire pigs were compared. Methods: RNA and protein were extracted from liver tissue of Tibetan and Yorkshire pigs (n = 3, each). Differentially expressed genes and proteins were subjected to gene ontology and Kyoto encyclopedia of genes and genomes functional enrichment analyses. Results: In the RNA-Seq and isobaric tags for relative and absolute quantitation analyses, a total of 18,791 genes and 3,390 proteins were detected and compared. Of these, 273 and 257 differentially expressed genes and proteins were identified. Evidence from functional enrichment analysis showed that many genes were involved in metabolic processes. The combined transcriptomic and proteomic analyses revealed that small molecular biosynthesis, metabolic processes, and organic hydroxyl compound metabolic processes were the major processes operating differently in the two breeds. The important genes include retinol dehydrogenase 16, adenine phosphoribosyltransferase, prenylcysteine oxidase 1, sorbin and SH3 domain containing 2, ENSSSCG00000036224, perilipin 2, ladinin 1, kynurenine aminotransferase 1, and dimethylarginine dimethylaminohydrolase 1. Conclusion: The findings of this study provide novel insight into the high-altitude metabolic adaptation of Tibetan pigs.

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References

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