Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Determination of Bergenin in Different Parts of Bergenia ciliata using a Validated RP-HPLC Method

  • Ali, Ejaz (Punjab University College of Pharmacy, University of the Punjab) ;
  • Hussain, Khalid (Punjab University College of Pharmacy, University of the Punjab) ;
  • Bukhari, Nadeem Irfan (Punjab University College of Pharmacy, University of the Punjab) ;
  • Arshad, Najma (Institute of Molecular Biology and Biotechnology, Centre for Research in Molecular Medicine, University of Lahore) ;
  • Hussain, Amjad (Punjab University College of Pharmacy, University of the Punjab) ;
  • Abbas, Nasir (Punjab University College of Pharmacy, University of the Punjab) ;
  • Arshad, Sohail (Faculty of Pharmacy, Baha Uddin Zakariya University) ;
  • Parveen, Sajida (Punjab University College of Pharmacy, University of the Punjab) ;
  • Shehzadi, Naureen (Punjab University College of Pharmacy, University of the Punjab) ;
  • Qamar, Shaista (Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences) ;
  • Qamar, Abida (Punjab University College of Pharmacy, University of the Punjab)
  • Received : 2021.02.15
  • Accepted : 2021.03.19
  • Published : 2021.03.31
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Abstract

Bergenia ciliata (Family: Saxifragaceae) is a folklore remedy for the treatment of various ailments in Asian countries. Bergenin (1) has been isolated as an active constituent in many studies, however, the amount of bergenin has not been determined in all parts of the plant. A simple RP-HPLC method was developed to determine the amount of bergenin in methanol extracts of leaves, rhizomes and roots of the plant. Separation was achieved on an Agilent Eclipse XDB-C18 column maintained at 25 ℃ using isocratic solvent system (water: methanol: acetic acid; 62.5:37:0.5 v/v/v) adjusted at pH 2 0 at a flow rate of 1.0 mL/min. and detected at 275 nm. Correlation coefficient (0.9952) showed linearity of concentration (5-200 ㎍/mL) and response. The values of LOD (0.00947 ㎍/mL) and LOQ (0.02869 ㎍/mL) indicated that method was sensitive. The recovery of bergenin was 99.99-100% indicating accuracy of method. The methanol extract of rhizomes contained higher amount of bergenin (19.4%) than roots (9.2%) and leaves (6.9%). It is concluded that methanol extract of rhizomes is a better source of bergenin than other parts of the plant. The findings are useful for standardization of bergenin containing extracts and herbal preparations.

Keywords

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