Journal of Korean Dental Science

Korean Academy of Dental Science (대한치의학회)
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Cytokine Release from Organotypic Human Oral Tissue Following Exposure to Oral Care Product Chemicals

  • Yang, Song-Yi (Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry) ;
  • Piao, Yin-Zhu (Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry) ;
  • Kim, Kwang-Mahn (Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry) ;
  • Kwon, Jae-Sung (Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry)
  • Received : 2021.05.07
  • Accepted : 2021.07.21
  • Published : 2021.12.30
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Abstract

Purpose: Measuring viability of a three-dimensional in vitro organotypic human oral tissue model has been suggested as an alternative test method to the oral mucosa irritation test of oral care products. The aim of this study was to investigate the production of two different cytokines using organotypic human oral tissue model following exposure to chemicals that are commonly used in oral care products. Materials and Methods: The organotypic human oral tissues were exposed to ethanol, sodium lauryl sulphate or hydrogen peroxide for 90 minutes. Following exposure, interleukin (IL)-1α and IL-8 productions were assessed and correlated with cell viability testing as well as histology of the organotypic human oral tissues. Result: High levels of IL-8 were released from organotypic human oral tissues in all of the test and control groups without any significant differences between them. In contrast, differences were found in IL-1α release between the test and control groups. Additionally, the trend of IL-1α release corresponded to the phenotypes observed in histological analysis while different trend existed between IL-1α release and cell viability. Conclusion: The study concluded the non-specific release of IL-8 for the assessment of oral care product chemicals' toxicity, while potential of measuring IL-1α cytokine level as the possible alternative test method.

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References

  1. de Souza Costa CA, Hebling J, Scheffel DL, Soares DG, Basso FG, Ribeiro AP. Methods to evaluate and strategies to improve the biocompatibility of dental materials and operative techniques. Dent Mater. 2014; 30: 769-84. https://doi.org/10.1016/j.dental.2014.04.010
  2. Hanks CT, Wataha JC, Sun Z. In vitro models of biocompatibility: a review. Dent Mater. 1996; 12: 186-93. https://doi.org/10.1016/S0109-5641(96)80020-0
  3. Wataha JC. Principles of biocompatibility for dental practitioners. J Prosthet Dent. 2001; 86: 203-9. https://doi.org/10.1067/mpr.2001.117056
  4. Vajrabhaya L, Sithisarn P. Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers. Int Endod J. 1997; 30: 141-4. https://doi.org/10.1046/j.1365-2591.1997.00056.x
  5. Williams DF. On the mechanisms of biocompatibility. Biomaterials. 2008; 29: 2941-53. https://doi.org/10.1016/j.biomaterials.2008.04.023
  6. Browne RM. The in vitro assessment of the cytotoxicity of dental materials--does it have a role? Int Endod J. 1988; 21: 50-8. https://doi.org/10.1111/j.1365-2591.1988.tb00955.x
  7. ISO (International Organization for Standardization). Biological evaluation of medical devices- part 10: tests for irritation and skin sensitization. ISO 10993-10. Geneva: International Organization for Standardization; 2010.
  8. Prieto P, Cole T, Curren R, Gibson RM, Liebsch M, Raabe H, Tuomainen AM, Whelan M, Kinsner-Ovaskainen A. Assessment of the predictive capacity of the 3T3 Neutral Red Uptake cytotoxicity test method to identify substances not classified for acute oral toxicity (LD50>2000 mg/kg): results of an ECVAM validation study. Regul Toxicol Pharmacol. 2013; 65: 344-65. https://doi.org/10.1016/j.yrtph.2012.11.013
  9. Moharamzadeh K, Brook IM, Van Noort R, Scutt AM, Thornhill MH. Tissue-engineered oral mucosa: a review of the scientific literature. J Dent Res. 2007; 86: 115-24. https://doi.org/10.1177/154405910708600203
  10. Vande Vannet BM, Hanssens JL. Cytotoxicity of two bonding adhesives assessed by three-dimensional cell culture. Angle Orthod. 2007; 77: 716-22. https://doi.org/10.2319/052706-212.1
  11. Vande Vannet B, Hanssens JL, Wehrbein H. The use of three-dimensional oral mucosa cell cultures to assess the toxicity of soldered and welded wires. Eur J Orthod. 2007; 29: 60-6. https://doi.org/10.1093/ejo/cjl063
  12. McGinley EL, Moran GP, Fleming GJ. Biocompatibility effects of indirect exposure of base-metal dental casting alloys to a human-derived three-dimensional oral mucosal model. J Dent. 2013; 41: 1091-100. https://doi.org/10.1016/j.jdent.2013.08.010
  13. Moharamzadeh K, Colley H, Murdoch C, Hearnden V, Chai WL, Brook IM, Thornhill MH, Macneil S. Tissue-engineered oral mucosa. J Dent Res. 2012; 91: 642-50. https://doi.org/10.1177/0022034511435702
  14. Klausner M, Ayehunie S, Breyfogle BA, Wertz PW, Bacca L, Kubilus J. Organotypic human oral tissue models for toxicological studies. Toxicol In Vitro. 2007; 21: 938-49. https://doi.org/10.1016/j.tiv.2007.01.024
  15. Koschier F, Kostrubsky V, Toole C, Gallo MA. In vitro effects of ethanol and mouthrinse on permeability in an oral buccal mucosal tissue construct. Food Chem Toxicol. 2011; 49: 2524-9. https://doi.org/10.1016/j.fct.2011.06.018
  16. Takahashi S, Abe T, Gotoh J, Fukuuchi Y. Substrate-dependence of reduction of MTT: a tetrazolium dye differs in cultured astroglia and neurons. Neurochem Int. 2002; 40: 441-8. https://doi.org/10.1016/S0197-0186(01)00097-3
  17. Kwon JS, Illeperuma RP, Kim J, Kim KM, Kim KN. Cytotoxicity evaluation of zinc oxide-eugenol and non-eugenol cements using different fibroblast cell lines. Acta Odontol Scand. 2014; 72: 64-70. https://doi.org/10.3109/00016357.2013.798871
  18. Kwon JS, Lee SB, Kim CK, Kim KN. Modified cytotoxicity evaluation of elastomeric impression materials while polymerizing with reduced exposure time. Acta Odontol Scand. 2012; 70: 597-602. https://doi.org/10.3109/00016357.2011.641127
  19. Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clin Oral Investig. 1997; 1: 154-62. https://doi.org/10.1007/s007840050027
  20. Schwarze T, Leyhausen G, Geurtsen W. Long-term cytocompatibility of various endodontic sealers using a new root canal model. J Endod. 2002; 28: 749-53. https://doi.org/10.1097/00004770-200211000-00001
  21. Roberta T, Federico M, Federica B, Antonietta CM, Sergio B, Ugo C. Study of the potential cytotoxicity of dental impression materials. Toxicol In Vitro. 2003; 17: 657-62. https://doi.org/10.1016/S0887-2333(03)00107-3
  22. Bednarkiewicz A, Rodrigues RM, Whelan MP. Non-invasive monitoring of cytotoxicity based on kinetic changes of cellular autofluorescence. Toxicol In Vitro. 2011; 25: 2088-94. https://doi.org/10.1016/j.tiv.2011.09.008
  23. Repetto G, del Peso A, Zurita JL. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc. 2008; 3: 1125-31. https://doi.org/10.1038/nprot.2008.75
  24. Baert JH, Veys RJ, Ampe K, De Boever JA. The effect of sodium lauryl sulphate and triclosan on hamster cheek pouch mucosa. Int J Exp Pathol. 1996; 77: 73-8. https://doi.org/10.1046/j.1365-2613.1996.00965.x
  25. Kwon JS, Kim KM, Kim KN. A comparative study of three cytotoxicity test methods for nanomaterials using sodium lauryl sulfate. J Nanosci Nanotechnol. 2014; 14: 8043-7. https://doi.org/10.1166/jnn.2014.9415
  26. Kwon JS, Lee SB, Kim KM, Kim KN. Positive control for cytotoxicity evaluation of dental vinyl polysiloxane impression materials using sodium lauryl sulfate. Acta Odontol Scand. 2014; 72: 618-22. https://doi.org/10.3109/00016357.2013.879996
  27. Moharamzadeh K, Franklin KL, Brook IM, van Noort R. Biologic assessment of antiseptic mouthwashes using a three-dimensional human oral mucosal model. J Periodontol. 2009; 80: 769-75. https://doi.org/10.1902/jop.2009.080610
  28. Gardner AM, Xu FH, Fady C, Jacoby FJ, Duffey DC, Tu Y, Lichtenstein A. Apoptotic vs. nonapoptotic cytotoxicity induced by hydrogen peroxide. Free Radic Biol Med. 1997; 22: 73-83. https://doi.org/10.1016/S0891-5849(96)00235-3
  29. Hanks CT, Fat JC, Wataha JC, Corcoran JF. Cytotoxicity and dentin permeability of carbamide peroxide and hydrogen peroxide vital bleaching materials, in vitro. J Dent Res. 1993; 72: 931-8. https://doi.org/10.1177/00220345930720051501
  30. Xu Q, Izumi K, Tobita T, Nakanishi Y, Feinberg SE. Constitutive release of cytokines by human oral keratinocytes in an organotypic culture. J Oral Maxillofac Surg. 2009; 67: 1256-64. https://doi.org/10.1016/j.joms.2009.02.003
  31. Sfakianakis A, Barr CE, Kreutzer DL. Actinobacillus actinomycetemcomitans-induced expression of IL-1alpha and IL-1beta in human gingival epithelial cells: role in IL-8 expression. Eur J Oral Sci. 2001; 109: 393-401. https://doi.org/10.1034/j.1600-0722.2001.00162.x
  32. Welk A, Rosin M, Ludtke C, Schwahn C, Kramer A, Daeschlein G. The peritoneal explant test for evaluating tissue tolerance to mouthrinses. Skin Pharmacol Physiol. 2007; 20: 162-6. https://doi.org/10.1159/000098703
  33. Gantenbein-Ritter B, Potier E, Zeiter S, van der Werf M, Sprecher CM, Ito K. Accuracy of three techniques to determine cell viability in 3D tissues or scaffolds. Tissue Eng Part C Methods. 2008; 14: 353-8. https://doi.org/10.1089/ten.tec.2008.0313
  34. Fisichella M, Dabboue H, Bhattacharyya S, Saboungi ML, Salvetat JP, Hevor T, Guerin M. Mesoporous silica nanoparticles enhance MTT formazan exocytosis in HeLa cells and astrocytes. Toxicol In Vitro. 2009; 23: 697-703. https://doi.org/10.1016/j.tiv.2009.02.007