The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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A Procedure for Inducing the Occurrence of Rice Seedling Blast in Paddy Field

  • Qin, Peng (College of Agronomy, Hunan Agricultural University) ;
  • Hu, Xiaochun (Key Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs, Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd.) ;
  • Jiang, Nan (College of Agronomy, Hunan Agricultural University) ;
  • Bai, Zhenan (Key Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs, Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd.) ;
  • Liu, Tiangang (Key Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs, Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd.) ;
  • Fu, Chenjian (Key Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs, Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd.) ;
  • Song, Yongbang (Key Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs, Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd.) ;
  • Wang, Kai (Key Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs, Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd.) ;
  • Yang, Yuanzhu (College of Agronomy, Hunan Agricultural University)
  • Received : 2020.12.01
  • Accepted : 2021.03.01
  • Published : 2021.04.01
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Abstract

Rice blast caused by the filamentous fungus Magnaporthe oryzae, is arguably the most devastating rice disease worldwide. Development of a high-throughput and reliable field blast resistance evaluation system is essential for resistant germplasm screening, resistance genes identification and resistant varieties breeding. However, the occurrence of rice blast in paddy field is easily affected by various factors, particularly lack of sufficient inoculum, which always leads to the non-uniform occurrence and reduced disease severity. Here, we described a procedure for adequately inducing the occurrence of rice seedling blast in paddy field, which involves pretreatment of diseased straw, initiation of seedling blast for the first batch of spreader population, inducing the occurrence of the second batch of spreader population and test materials. This procedure enables uniform and consistent infection, which facilitates efficient and accurate assessment of seedling blast resistance for diverse rice materials.

Keywords

References

  1. Calvero, J. S. Jr., Coakley, S. M. and Teng, P. S. 1996. Development of empirical forecasting models for rice blast based on weather factors. Plant Pathol. 45:667-678. https://doi.org/10.1046/j.1365-3059.1996.d01-168.x
  2. Fones, H. N., Bebber, D. P., Chaloner, T. M., Kay, W. T., Steinberg, G. and Gurr, S. J. 2020. Threats to global food security from emerging fungal and oomycete crop pathogens. Nat. Food 1:332-342. https://doi.org/10.1038/s43016-020-0075-0
  3. Hunan Insititute of Plant Protection. 2006. Methods of identification and evaluation of rice resistance to Magnaporthe grisea. Administration for Quality and Technology Supervision of Hunan Province, Changsha, China. 7 pp.
  4. Jiang, H., Feng, Y., Bao, L., Li, X., Gao, G., Zhang, Q., Xiao, J., Xu, C. and He, Y. 2012. Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding. Mol. Breed. 30:1679-1688. https://doi.org/10.1007/s11032-012-9751-6
  5. Jiang, H., Feng, Y., Qiu, L., Gao, G., Zhang, Q. and He, Y. 2020. Identification of blast resistance QTLs based on two advanced backcross populations in rice. Rice 13:31. https://doi.org/10.1186/s12284-020-00392-6
  6. Katsantonis, D., Kadoglidou, K., Dramalis, C. and Puigdollers, P. 2017. Rice blast forecasting models and their practical value: a review. Phytopathol. Mediterr. 56:187-216.
  7. Liang, Y., Yan, B.-Y., Peng, Y.-L., Ji, Z.-J., Zeng, Y.-X., Wu, H.-L. and Yang, C.-D. 2017. Molecular screening of blast resistance genes in rice germplasms resistant to Magnaporthe oryzae. Rice Sci. 24:41-47. https://doi.org/10.1016/j.rsci.2016.07.004
  8. Peng, Z., Liu, Y., He, Q., Tan, Y., Pei, Y., Deng, H. and Xing, J. 2019. Population diversity and variation of the rice blast fungus under different climate conditions. Eur. J. Plant Pathol. 155:881-889. https://doi.org/10.1007/s10658-019-01819-7
  9. Raveloson, H., Ratsimiala Ramonta, I., Tharreau, D. and Sester, M. 2017. Long-term survival of blast pathogen in infected rice residues as major source of primary inoculum in high altitude upland ecology. Plant Pathol. 67:610-618. https://doi.org/10.1111/ppa.12790
  10. Skamnioti, P. and Gurr, S. J. 2009. Against the grain: safeguarding rice from rice blast disease. Trends Biotechnol. 27:141-150. https://doi.org/10.1016/j.tibtech.2008.12.002
  11. Sun, P., Liu, J., Wang, Y., Jiang, N., Wang, S., Dai, Y., Gao, J., Li, Z., Pan, S., Wang, D., Li, W., Liu, X., Xiao, Y., Liu, E., Wang, G.-L. and Dai, L. 2013. Molecular mapping of the blast resistance gene Pi49 in the durably resistant rice cultivar Mowanggu. Euphytica 192:45-54. https://doi.org/10.1007/s10681-012-0829-3
  12. Talbot, N. J. 2003. On the trail of a cereal killer: exploring the biology of Magnaporthe grisea. Annu. Rev. Microbiol. 57:177-202. https://doi.org/10.1146/annurev.micro.57.030502.090957
  13. Vasudevan, K., Vera Cruz, C. M., Gruissem, W. and Bhullar, N. K. 2014. Large scale germplasm screening for identification of novel rice blast resistance sources. Front. Plant Sci. 5:505.