Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Tsg101 Is Necessary for the Establishment and Maintenance of Mouse Retinal Pigment Epithelial Cell Polarity

  • Le, Dai (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Lim, Soyeon (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Min, Kwang Wook (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Park, Joon Woo (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Kim, Youjoung (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Ha, Taejeong (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Moon, Kyeong Hwan (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Wagner, Kay-Uwe (Department of Oncology, Wayne State University) ;
  • Kim, Jin Woo (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
  • Received : 2021.02.02
  • Accepted : 2021.03.02
  • Published : 2021.03.31
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Abstract

The retinal pigment epithelium (RPE) forms a monolayer sheet separating the retina and choroid in vertebrate eyes. The polarized nature of RPE is maintained by distributing membrane proteins differentially along apico-basal axis. We found the distributions of these proteins differ in embryonic, post-natal, and mature mouse RPE, suggesting developmental regulation of protein trafficking. Thus, we deleted tumor susceptibility gene 101 (Tsg101), a key component of endosomal sorting complexes required for transport (ESCRT), in embryonic and mature RPE to determine whether ESCRT-mediated endocytic protein trafficking correlated with the establishment and maintenance of RPE polarity. Loss of Tsg101 severely disturbed the polarity of RPE, which forms irregular aggregates exhibiting non-polarized distribution of cell adhesion proteins and activation of epidermal growth factor receptor signaling. These findings suggest that ESCRT-mediated protein trafficking is essential for the development and maintenance of RPE cell polarity.

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