Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Antibacterial Activity of Streptomyces sp. J46 against Bacterial Shot Hole Disease Pathogen Xanthomonas arboricola pv. pruni

Streptomyces sp. J46의 세균성구멍병원균 Xanthomonas arboricola pv. pruni에 대한 항균 활성

  • Lee, Jeong Eun (Department of Agricultural Chemistry, Chonnam National University) ;
  • Lim, Da Jung (Department of Agricultural Chemistry, Chonnam National University) ;
  • Kim, In Seon (Department of Agricultural Chemistry, Chonnam National University)
  • Received : 2021.03.02
  • Accepted : 2021.03.12
  • Published : 2021.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

BACKGROUND: Bacterial shot hole of stone fruits is a seriuos plant disease caused by Xanthomonas arboricola pv. pruni (Xap). Techniques to control the disease are required. In this study, microorganisms with antibacterial activity were isolated to develop as a microbial agent against the bacterial shot hole. METHODS AND RESULTS: An isolate with the strongest activity among the isolates was identified as Streptomyces avidinii based on 16S rRNA gene sequence analysis and designated Streptomyces sp. J46. J46 showed suppression of bacterial leaf spot with a control value of 90% at 10 times-diluted cell free supernatant. To investigate antibacterial metabolites produced by J46, the supernatant of J46 was extracted with organic solvents, and the extracts were subjected to chromatography works. Antibacterial metabolites were not extractable with organic solvents. Both reverse and normal phase techniques were not successful because the metabolites were extremely water soluble. The antibacterial metabolites were not volatiles but protein compounds based on hydrolysis enzyme treatment. CONCLUSION: Our study suggests that Streptomyces sp. J46 may be a potential as an microbial agent against bacterial shot hole. Further study to identify the metabolites is required in more detail.

Keywords

References

  1. Zotchev SB (2012) Marine actinomycetes as an emerging resource for the drug development pipelines. Journal of Biotechnology, 158, 168-175. https://doi.org/10.1016/j.jbiotec.2011.06.002.
  2. Miles WG, Daines RH and Rue JW (1977) Presymptomatic egress of Xanthomonas pruni from infected peach leaves. Phytopathology, 67, 895-897. https://doi.org/10.1094/Phyto-67-895
  3. Rolfs FM (1915) Bacterial disease of stone fruits. Cornell University Agriculture Experimental Station Memoir, 8, 381-436.
  4. Kim SY, Kwon TY, Kim IS, Choi SY, Choi CD, Em JY (2001) Protection of peach trees from bacterial shot hole with bordeaux mixture spray during the postharvest season. Research in Plant Disease, 7, 37-41. https://doi.org/10.5423/RPD.2008.14.3.182.
  5. Choi J, Lee E and Park Y (2000) Shot hole of peach and Japanese plum caused by Xanthomonas campestris pv. pruni and Erwinia nigrifluens in Korea. Research in Plant Disease, 6, 10-14.
  6. EPPO/OEPP. 2006. Data sheets on quarantine pests. Xanthomonas arboricola pv. pruni. http://www.eppo.org/QUARANTINE/listA2.htm.
  7. Kim CH (2000) Review of fungicide resistance problems in Korea. Korean Journal of Pesticide Science, 4, 1-10.
  8. Ritchie D (2004) Copper-containing fungicides/bactericides and their use in management of bacterial spot on peaches. Southeast Regional Newsletter, 4, 1-3.
  9. Atkinson NJ and Urwin PE (2012) The interaction of plant biotic and abiotic stresses: from genes to the field. Journal of Experimental Botany, 63, 3523-3543. https://doi.org/10.1093/jxb/ers100.
  10. Miyadoh S (1993) Research on antibiotic screening in Japan over the last decade: a producing microorganism approach. Actinomycetologica, 7, 100-106. https://doi.org/10.3209/saj.7-100.
  11. Umezawa H (1965) A new antibiotic, kasugamycin. The Journal of Antibiotics, 18, 101-103.
  12. Takeuchi S, Hirayama K, Ueda K, Sakai H and Yonehara H (1958) Blasticidin S, a new antibiotic. The Journal of Antibiotics, 11, 1-5.
  13. Timoshchuk VA, Kulinkovich LN, Olimpieva TI, Boreko EI and Vladyko GV (1988) Synthesis of nucleosides of uronic acids V. synthesis and antivirus activity of nucleosides of D-xyluronic acid. Pharmaceutical Chemistry Journal, 22, 41-49. https://doi.org/10.1007/BF00759055
  14. Endo A and Misato T (1969) Polyoxin D, a competitive inhibitor of UDP-N-acetylglucosamine: chitin N-acetylglucosaminyltransferase in Neurospora crassa. Biochemical and Biophysical Research Communications, 37, 718-722. https://doi.org/10.1016/0006-291X(69)90870-5.
  15. Isono K, Nagatsu J, Kawashima Y and Suzuki S (1965) Studies on polyoxins, antifungal antibiotics: Part I. Isolation and characterization of Polyoxins A and B. Agricultural and Biological Chemistry, 29, 848-854. https://doi.org/10.1080/00021369.1965.10858475.
  16. Kim IS, Ryu JY, Hur HG, Gu MB, Kim SD, Shim JH (2004) Sphingomonas sp. strain SB5 degrades carbofuran to a new metabolite by hydrolysis at the furanyl ring. Journal of Agricultural and Food Chemistry, 52, 2309-2314. https://doi.org/10.1021/jf035502l.
  17. Herrmann Jr EC, Gabliks J, Engle C and Perlman PL (1960) Agar diffusion method for detection and bioassay of antiviral antibiotics. Experimental Biology and Medicine, 103, 625-628. https://doi.org/10.3181/00379727-103-25617.
  18. Randhawa PS and Civerolo EL (1985) A detached-leaf bioassay for Xanthomonas campestris pv. pruni. Phytopathology, 75, 1060-1063. https://doi.org/10.1094/Phyto-75-1060.
  19. Suter M, Cazin Jr J, Butler JE and Mock DM (1988) Isolation and characterization of highly purified streptavidin obtained in a two-step purification procedure from Streptomyces avidinii grown in a synthetic medium. Journal of Immunological Methods, 113, 83-91. https://doi.org/10.1016/0022-1759(88)90384-5.
  20. Morgan TD, Oppert B, Czapla TH and Kramer KJ (1993) Avidin and streptavidin as insecticidal and growth inhibiting dietary proteins. Entomologia Experimentalis et Applicata, 69, 97-108. https://doi.org/10.1111/j.1570-7458.1993.tb01733.x.
  21. Da Silva Vasconcellos FC, De oliveira AG, Lopes-Santos L, Cely MVT, Simionato AS, Pistori JF and Andrade G (2014) Evaluation of antibiotic activity produced by Pseudomonas aeruginosa LV strain against Xanthomonas arboricola pv. pruni. Agricultural Sciences, 5, 71-76. https://doi.org/10.4236/AS.2014.51008.
  22. Kawaguchi A, Inoue K and Inoue Y (2014) Biological control of bacterial spot on peach by nonpathogenic Xanthomonas campestris strains AZ98101 and AZ98106. Journal of General Plant Pathology, 80, 158-163. https://doi.org/10.1007/s10327-014-0506-6.