Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Determination of isovitexin from Lespedeza cuneata using a validated HPLC-UV method

  • Lee, Ju Sung (Department of Plant Science and Technology, Chung-Ang University) ;
  • Paje, Leo Adrianne (Department of Plant Science and Technology, Chung-Ang University) ;
  • Yoo, Sang-Woo (Research & Development Center, Natural Way Co., Ltd.) ;
  • Lee, Seong (Dankook University Hospital Research Institute of Clinical Medicine) ;
  • Ku, Ja-Jung (Forest Bioinformation Division, National Institute of Forest Science) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2020.10.07
  • Accepted : 2021.01.18
  • Published : 2021.03.31
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Abstract

Isovitexin, a marker compound with various pharmacological activities, in Lespedeza cuneata, was analyzed using high performance liquid chromatography coupled with UV (HPLC/UV). There are no previous reports on using L. cuneata as the source material for the quantification of isovitexin. In this study, we developed an optimized method using HPLC-UV analysis, which was validated using various parameters. Our method demonstrated high specificity, and good separation of the chromatographic peak was achieved. Parameters such as linearity (r2≥0.9997), precision, and accuracy indicated that our proposed analytical method had good reliability and sensitivity. These results demonstrate the utility and convenience of our method for rapidly quantifying isovitexin in L. cuneata extracts.

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References

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