Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Neuroprotective and anti-inflammatory activity of marine sponge extract and HPLC profiling of its components

해면 추출물의 신경세포 보호 및 항염증 활성과 함유 성분의 HPLC 프로파일링

  • Kim, Da-Eun (Natural Product Informatics Research Center, Korea Institute of Science and Technology) ;
  • Kim, Min-Seon (Natural Product Informatics Research Center, Korea Institute of Science and Technology) ;
  • An, Hye Suck (Marine Biology Research Division, National Marine Biodiversity Institute of Korea) ;
  • Lee, Jae Wook (Natural Product Research Center, Korea Institute of Science and Technology) ;
  • Park, Jin-Soo (Natural Product Informatics Research Center, Korea Institute of Science and Technology)
  • Received : 2020.12.24
  • Accepted : 2020.12.30
  • Published : 2021.03.31
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Abstract

Marine sponges contain pharmacologically attractive substances that exhibit strong cytotoxicity and are used as materials to isolate potential drug candidates. However, with a growing interest in marine ecosystem conservation, it is becoming increasingly difficult to gather a sponge for natural product research. To build a database to cope with this issue, we measured the neuroprotective and anti-inflammatory activity of 181 sponge extracts. As a result, we found 17 samples with neuroprotective effects and 14 samples with anti-inflammatory effects. In addition, high-performance liquid chromatography (HPLC) analysis was performed to compare the components contained in each sample, and based on HPLC profiles, a dendrogram according to similarity was created. The results of this study suggested the possibility of discovering the active compounds in the sponge and laid the basis for efficient research on the sponge.

해면동물은 강력한 세포독성을 나타내는 함유성분으로 의약후보물질 탐색의 중요한 소재로 이용되고 있으나 최근 해양생태계 보전에 관심이 높아짐에 따라 해면동물 확보는 더욱 어려움을 겪고 있다. 이러한 경향에 대응하고 해양 천연물연구의 활성화를 위한 해양생물자원 데이터베이스 구축을 위하여 181개 해면동물 추출물에 대한 HPLC 프로파일을 생성하고 항염증 및 신경세포보호 활성을 탐색하였다. 이를 통하여 해면동물 추출물 간의 HPLC를 통한 함유성분에 따른 클러스터링이 가능하였고 또한 각각 17개, 14개 시료에서 항염증 활성과 신경세포보호활성이 확인되었다. 이와 같은 연구는 해면동물을 포함한 해양생물자원에서의 효과적인 생리활성 탐색에 도움이 될 것이라고 판단한다.

Keywords

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