Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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1-Methoxylespeflorin G11 Protects HT22 Cells from Glutamate-Induced Cell Death through Inhibition of ROS Production and Apoptosis

  • Lee, Phil Jun (College of Pharmacy and Research Institute of Pharmaceutical Science and Technology, Ajou University) ;
  • Pham, Chau Ha (Biometrology Group, Korea Research Institute of Standards and Science (KRISS)) ;
  • Thuy, Nguyen Thi Thanh (College of Pharmacy and Research Institute of Drug Development, Chonnam National University) ;
  • Park, Hye-Jin (College of Pharmacy and Research Institute of Pharmaceutical Science and Technology, Ajou University) ;
  • Lee, Sung Hoon (College of Pharmacy, Chung-Ang University) ;
  • Yoo, Hee Min (Biometrology Group, Korea Research Institute of Standards and Science (KRISS)) ;
  • Cho, Namki (College of Pharmacy and Research Institute of Drug Development, Chonnam National University)
  • Received : 2020.11.23
  • Accepted : 2020.12.28
  • Published : 2021.02.28
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Abstract

This study aimed to investigate the neuroprotective effects of 1-methoxylespeflorin G11 (MLG), a pterocarpan, against glutamate-induced neurotoxicity in neuronal HT22 hippocampal cells. The protective effects of MLG were evaluated using MTT assay and microscopic analysis. The extent of apoptosis was studied using flow cytometric analysis performed on the damaged cells probed with annexin V/propidium iodide. Moreover, mitochondrial reactive oxygen species (ROS) were assessed using flow cytometry through MitoSOXTM Red staining. To determine mitochondrial membrane potential, staining with tetramethylrhodamine and JC-1 was performed followed by flow cytometry. The results demonstrated that MLG attenuates glutamate-induced apoptosis in HT22 cells by inhibiting intracellular ROS generation and mitochondrial dysfunction. Additionally, MLG prevented glutamate-induced apoptotic pathway in HT22 cells through upregulation of Bcl-2 and downregulation of cleaved PARP-1, AIF, and phosphorylated MAPK cascades. In addition, MLG treatment induced HO-1 expression in HT22 cells. These results suggested that MLG exhibits neuroprotective effects against glutamate-induced neurotoxicity in neuronal HT22 cells by inhibiting oxidative stress and apoptosis.

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References

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