Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Evaluation of different molecular methods for detection of Senecavirus A and the result of the antigen surveillance in Korea during 2018

  • Heo, JinHwa (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Lee, Min-Jung (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Kim, HyunJoo (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Lee, SuKyung (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Choi, Jida (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Kang, Hae-Eun (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Nam, Hyang-Mi (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Nah, JinJu (Foreign Animal Disease Division, Animal and Plant Quarantine Agency)
  • Received : 2020.12.31
  • Accepted : 2021.03.23
  • Published : 2021.03.30
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Abstract

Senecavirus A (SVA), previously known as Seneca Valley virus, can cause vesicular disease and neonatal losses in pigs that is clinically indistinguishable from foot-and-mouth disease virus (FMDV). After the first case report in Canada in 2007, it had been restrictively identified in North America including United States. But, since 2015, SVA emerged outside North America in Brazil, and also in several the Asian countries including China, Thailand, and Vietnam. Considering the SVA occurrence in neighboring countries, there has been a high risk that Korea can be introduced at any time. In particular, it is very important in terms of differential diagnosis in the suspected case of vesicular diseases in countries where FMD is occurring. So far, several different molecular detection methods for SVV have been published but not validated as the reference method, yet. In this study, seven different molecular methods for detecting SVA were evaluated. Among them, the method by Flowler et al, (2017) targeted to 3D gene region with the highest sensitivity and no cross reaction with other vesicular disease agents including FMDV, VSV and SVD, was selected and applied further to antigen surveillance of SVA. A total of 245 samples of 157 pigs from 61 farms submitted for animal disease diagnose nationwide during 2018 were tested all negative. In 2018, no sign of SVA occurrence have been confirmed in Korea, but the results of the surveillance for SVA needs to be continued and accumulated at a high risk of SVA in neighboring countries.

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References

  1. Agnol AMD, Otonel RAA, Leme RA, Alfieri AA, Alfieri AF. 2017. A TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets. Molecular and Cellular Probes 33: 28-31. https://doi.org/10.1016/j.mcp.2017.03.002
  2. Arzt J, Bertram MR, Vu LT, Pauszek SJ, Hartwig EJ, Smoliga GR, Phuong NT. 2019. First detection and genome sequence of Senecavirus A in Vietnam. Microbiology Resource Announcements 8(3).
  3. Bracht AJ, O'Hearn ES, Fabian AW, Barrette RW, Sayed A. 2016. Real-time reverse transcription PCR assay for detection of Senecavirus A in swine vesicular diagnostic specimens. PLoS One 11(1): e0146211. https://doi.org/10.1371/journal.pone.0146211
  4. Feronato C, Leme RA, Diniz JA, Agnol AMD, Alfieri AF, Alfieri AA. 2018. Development and evaluation of a nested-PCR assay for Senecavirus A diagnosis. Tropical Animal Health and Production 50(2): 337-344. https://doi.org/10.1007/s11250-017-1436-z
  5. Fowler VL, Ransburgh RH, Poulsen EG, Wadsworth J, King DP, Mioulet V, Fang Y. 2017. Development of a novel real-time RT-PCR assay to detect Seneca Valley virus-1 associated with emerging cases of vesicular disease in pigs. Journal of Virological Methods 239: 34-37. https://doi.org/10.1016/j.jviromet.2016.10.012
  6. Joshi LR, Mohr KA, Clement T, Hain KS, Myers B, Yaros J, Caron L. 2016. Detection of the emerging picornavirus Senecavirus A in pigs, mice, and houseflies. Journal of Clinical Microbiology 54(6): 1536-1545. https://doi.org/10.1128/JCM.03390-15
  7. Knowles NJ, Hales LM, Jones BH, Landgraf JG, House JA, Skele KL, Hallenbeck PL. 2006. Epidemiology of Seneca Valley virus: identification and characterization of isolates from pigs in the United States. Northern Lights EUROPIC.
  8. Leme RA, Alfieri AF, Alfieri AA. 2017. Update on Senecavirus infection in pigs. Viruses 9(7): 170. https://doi.org/10.3390/v9070170
  9. Leme RA, Oliveira TE, Alcantara BK, Headley SA, Alfieri AF, Yang M, Alfieri AA. 2016. Clinical manifestations of Senecavirus A infection in neonatal pigs, Brazil, 2015. Emerging Infectious Diseases 22(7): 1238. https://doi.org/10.3201/eid2207.151583
  10. Leme RA, Zotti E, Alcantara BK, Oliveira MV, Freitas LA, Alfieri AF, Alfieri AA. 2015. Senecavirus A: an emerging vesicular infection in Brazilian pig herds. Transboundary and Emerging Diseases 62(6): 603-611. https://doi.org/10.1111/tbed.12430
  11. Liu F, Wang Q, Huang Y, Wang N, Shan H. 2020. A 5-year Review of Senecavirus A in China since Its Emergence in 2015. Frontiers in Veterinary Science 7.
  12. Maggioli MF, Lawson S, de Lima M, Joshi LR, Faccin TC, Bauermann FV, Diel DG. 2018. Adaptive immune responses following Senecavirus A infection in pigs. Journal of Virology 92(3).
  13. Pasma T, Davidson S, Shaw SL. 2008. Idiopathic vesicular disease in swine in Manitoba. The Canadian Veterinary Journal 49(1): 84.
  14. Rudin CM, Poirier JT, Senzer NN, Stephenson J, Loesch D, Burroughs KD, Hallenbeck PL. 2011. Phase I clinical study of Seneca Valley Virus (SVV-001), a replicationcompetent picornavirus, in advanced solid tumors with neuroendocrine features. Clinical Cancer Research 17(4): 888-895. https://doi.org/10.1158/1078-0432.CCR-10-1706
  15. Saeng-Chuto K, Rodtian P, Temeeyasen G, Wegner M, Nilubol D. 2018. The first detection of Senecavirus A in pigs in Thailand, 2016. Transboundary and Emerging Diseases 65(1): 285-288. https://doi.org/10.1111/tbed.12654
  16. Wu Q, Zhao X, Chen Y, He X, Zhang G, Ma J. 2016. Complete genome sequence of Seneca Valley virus CH-01-2015 identified in China. Genome Announcements 4(1).
  17. Zhang X, Xiao J, Ba L, Wang F, Gao D, Zhang J, Qi P. 2018. Identification and genomic characterization of the emerging Senecavirus A in southeast China, 2017. Transboundary and Emerging Diseases 65(2): 297-302. https://doi.org/10.1111/tbed.12750