Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Development of HPLC Method for Quality Assessment of Marker Components in Atractylodis Rhizoma Alba

백출(Atractylodis Rhizoma Alba)의 품질평가를 위한 지표성분 분석법 평가

  • Lee, Jae-Woong (Medicinal Crops Seed Supply Center, National Development Institute of Korean Medicine) ;
  • Kim, Joon-Hee (Medicinal Crops Seed Supply Center, National Development Institute of Korean Medicine) ;
  • Kang, Byoung-Man (Medicinal Crops Seed Supply Center, National Development Institute of Korean Medicine) ;
  • Ahn, Byung-Kwan (Medicinal Crops Seed Supply Center, National Development Institute of Korean Medicine)
  • 이재웅 (한국한의약진흥원 한약자원개발본부 약용작물종자보급센터) ;
  • 김준희 (한국한의약진흥원 한약자원개발본부 약용작물종자보급센터) ;
  • 강병만 (한국한의약진흥원 한약자원개발본부 약용작물종자보급센터) ;
  • 안병관 (한국한의약진흥원 한약자원개발본부 약용작물종자보급센터)
  • Received : 2020.07.27
  • Accepted : 2020.11.05
  • Published : 2021.02.01
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Abstract

Homogenization of quality was important in order to use herbal medicines as pharmaceuticals. To solve this problem, it was important to establish quality standards. Atractylodis Rhizoma Alba has no quantitative method in the Korean Pharmacopoeia. Thus, we have researched to improve the quality evaluation method of Atractylodis Rhizoma Alba with an HPLC. Atractylenolide III and atracylodin were selected as potential marker compounds. This analytical procedure was subject to validation. According to validation guideline of South Korea's Ministry of Food and Drugs Safety, the specificity, linearity, precision, range, quantitative limits, detection limits and accuracy were measured. Because the specificity, linearity, precision, range, quantitative limits, detection limits and accuracy meet criteria of the guideline, the analytic method was validated. With this analysis, Atractylodis Rhizoma Alba and Atractylodis Rhizoma analyzed. As a result, both atractylenolide III and atracylodin appear to be suitable standard compounds. it confirmed that tractylodes Rhizome, similar to Atractylodes Rhizome Alba, could be distinguished.

백출의 기원 감별 및 품질기준 설정을 위하여 atractylenolide III과 atracylodin을 지표성분으로 설정하였다. HPLC를 이용하여 선정된 지표물질인 atractylenolide III과 atracylodin의 특이성, 직선성, 범위, 검출한계, 정량한계, 정확성, 정밀성에 대한 평가를 실시한 결과 모두 양호한 결과가 나타나 분석법에 문제는 없었으며, 백출과 창출을 각각 분석한 결과 위품인 창출에서만 atracylodin이 검출이 되어 위품 구별이 가능한 지표물질로 손색이 없었다.

Keywords

References

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