Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-inflammatory Effect of Berchemia berchemiaefolia Leaves through Inhibition of NF-κB and MAPK Signaling Activation in LPS-stimulated RAW264.7 Cells

망개나무 추출물의 NF-κB 및 MAPK 신호전달 억제를 통한 항염증 효과

  • Kim, Da Som (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Eo, Hyun Ji (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Kang, Yeongyeong (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Park, Gwang Hun (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 김다솜 (국립산림과학원 산림약용자원연구소) ;
  • 어현지 (국립산림과학원 산림약용자원연구소) ;
  • 강연경 (국립산림과학원 산림약용자원연구소) ;
  • 박광훈 (국립산림과학원 산림약용자원연구소)
  • Received : 2020.09.10
  • Accepted : 2020.12.15
  • Published : 2021.02.01
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Abstract

Berchemia berchemiaefolia (Makino) Koidz. is found not only in korea, but also in japan and is known as a rare plant all over the world. In this study, we evaluated anti-inflammatory effect of B. berchemiaefolia (Makino) Koidz. leaves (BBK-L) in LPS-stimulated RAW264.7 cells. BBK-L inhibited the generation of NO through the suppression of iNOS experession. BBK-L attenuated the expression of iNOS, COX-2, TNF-α, IL-1β, IL-6 induced by LPS. BBK-L decreased LPS-mediated IκB-α degradation inhibite which resulted in the inhibition of NF-κB activation in RAW264.7 cells. In addition, BBK-L suppressed ERK1/2, JNK phosphorylation induced by LPS. BBK-L showed anti-inflammatory effect through blocking the generation of the inflammatory mediators such as NO, iNOS, COX-2, TNF-α, IL-1β, IL-6 via the inhibiting of NF-κB and MAPK signaling activation. These results suggests that BBK-L may have great potential for the development of anti-inflammatory drug to treat acute and chronic inflammatory disorders.

본 연구는 망개나무 추출물의 항염증 효과를 구명하기 위하여 수행되었다. 망개나무를 이용한 다양한 기능성 연구가 진행되고 있으나 망개나무 항염증에 대한 연구는 과학적 근거가 부족한 실정이다. 따라서 망개나무 잎(BBK-L) 추출물이 마우스 대식세포인 RAW264.7 세포에서 LPS에 의한 염증반응에 미치는 영향을 평가하였다. LPS로 유도된 마우스 대식세포 RAW264.7에 BBK-L 추출물을 농도별로 처리하였을 때, NO 생성량과 만성염증 유발인자인 iNOS, COX-2, IL-1β, TNF-α및 IL-6 발현 감소를 확인하였으며, NF-κB 및 MAPK의 인산화를 억제하는 것을 확인하였다. 이러한 연구결과를 토대로 BBK-L 추출물이 만성 염증질환의 예방과 치료 또는 기능성 소재로 활용될 수 있는 기초적인 정보를 제공할 수 있을 것으로 사료된다.

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