The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Comparative evaluation on biological activities of Ziingiber officinale Roscoe extracted from different solvents

추출 용매를 달리한 생강 추출물에 대한 생리활성의 비교 평가 연구

  • Kim, So Young (Department of Herbal Pharmacology, College of Oriental Medicine, Daegu Haany University) ;
  • Choi, Moon-Yeol (Department of Herbal Pharmacology, College of Oriental Medicine, Daegu Haany University) ;
  • Do, Eun ju (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, College of Oriental Medicine, Daegu Haany University)
  • 김소영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 최문열 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 도은주 ((재)대구테크노파크 한방산업지원센터) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2021.02.10
  • Accepted : 2021.03.25
  • Published : 2021.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study was designed to investigate anti-oxidative, anti-aging and anti-inflammatory activities of Zingiber officinale Roscoe water (ZD) and 50% ethanol extracts (ZE). Method : The antioxidant capacity of ZD or ZE was analyzed by DPPH, ABTS scavenging activities and reducing power, respectively. The anti-aging efficacy was performed with tyrosinase and collagenase inhibition activities. The anti-inflammatory activities were confirmed through inhibition of nitric oxide production and TNF-�� expression in LPS-treated Raw 264.7 cells. Result : As a result of measuring DPPH and ABTS radical scavenging activities, both ZD and ZE extracts increased in concentration-dependent manners. The ABTS radical scavenging activities of water and 50% ethanol extract were similar to that of positive control at high concentration. On reducing power, both ZD and ZE showed higher activities than the positive control at high concentration. On inhibition activities of tyrosinase and collagenase both ZD and ZE showed the large inhibition rates at low concentration. Also, the inhibitory efficacy of ZD on NO and TNF-�� production was better than ZE in RAW 264.7 cells. Conclusion : These results suggest that ZD and ZE have anti-oxidative, anti-aging and anti-inflammatory effects in vitro. Especially, ZD was more effective than ZE. Thus, Ginger extract is considered to be highly useful as a cosmetic or food material with anti-oxidative, anti-aging (prevention of whitening and wrinkle) and anti-inflammatory effects. Also these effects may vary depending on the extraction solvent.

Keywords

References

  1. Kim MS. A study on the effects of depression caused by skin condition on life stress and quality of life. J Kor Soc Cosm. 2017 ; 23(4) : 739-52.
  2. Seo JY, Cho KH, Eun HC, Chung JH. Skin aging from phenotype to mechanism. Ko. J. Invest. Dermatol. 2001 ; 8(4) : 187-94.
  3. Edwin DL. Skin aging and oxidative stress: Equol's anti-aging effects via biochemical and molecular mechanisms. Ageing Res Rev. 2016 ; 31 : 36-54. https://doi.org/10.1016/j.arr.2016.08.001
  4. Rossetti D, Kielmanowicz MG, Vigodman S, Hu YP, Chen N, Nkengne A, Oddos T, Fischer D, Seiberg M, Lin CB. A novel anti-ageing mechanism for retinol: induction of dermal elastin synthesis and elastin fibre formation. Int J Cosmet Sci. 2011 ; 33 : 62-9. https://doi.org/10.1111/j.1468-2494.2010.00588.x
  5. Gonzaga ER. Role of UV light in photoaging, skin aging, and skin cancer. Am. J. Clin. Dermatol. 2009 ; 10(1) : 19-24. https://doi.org/10.2165/0128071-200910001-00004
  6. Fischer SM, Pavone A, Mikulec C, Langenbach R, Rundhaug JE. Cyclooxygenase-2 expression is critical for chronic UV-induced murine skin carcinogenesis. Mol Carcinog. 2007 ; 46(5) : 363-71. https://doi.org/10.1002/mc.20284
  7. Chun KS, Cha HH, Shin JW, Na H., Park KK, Chung WY, Surh YJ. Nitric oxide induces expression of cyclooxygenase-2 in mouse skin through activation of NF-κB. Carcinogenesis. 2004 ; 25(3) : 445-54. https://doi.org/10.1093/carcin/bgh021
  8. Kim JS, Koh MS, Kim YH, Kim MK, Hong JS. Volatile flavor components of korean ginger (Zingiber officinale Roscoe). Korean J Food Sci Technol. 1991 ; 23 : 141-9.
  9. Mao QQ, Xu XY, Cao SY, Gan RY, Corke H, Beta T, Li HB. Bioactive compounds and bioactivities of ginger (Zingiber ocinale Roscoe). Foods. 2019 ; 8(6) : 1-21.
  10. Connell DW. The chemistry of the essential oil and oleoresin of ginger (Zingiber officinale Roscoe). Flavour Industry. 1970 ; 1 : 677-93.
  11. Yang WK, Jung CS, Jung KW, Kim JW, Lee EB. Antigastric and antiulcerative action of the extract of Zingiberis Rhizoma. Yakhak Hoeji. 1992 ; 36 : 173-79.
  12. Yamahara J, Huang Q, Li Y, Xu L, Fujimura H. Gastrointestinal motility enhancing effect of ginger and its active constituents. Chem Pharm Bull. 1990 ; 38(2) : 430-1. https://doi.org/10.1248/cpb.38.430
  13. Sheo HJ. The antibacterial action of garlic, onion, ginger and red pepper juice. J Kor Soc Food Sci Nutr. 1999 ; 28 : 94-9.
  14. Ali AMA, El-Nour MEM, Mohammad O, Yagi SM. In vitro anti-inflammatory activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors. J Med Plants Res. 2019 ; 13(10) : 227-35.
  15. Shukla Y, Singh M. Cancer preventive properties of ginger: a brief review. Food Chem Toxicol. 2007 ; 45(5) : 683-90. https://doi.org/10.1016/j.fct.2006.11.002
  16. Bae JS, Kim TH. Pancreatic lipase inhibitory and antioxidant activities of Zingiber officinale extracts. Kor J Food Preserv. 2011 ; 18(3) : 390-6. https://doi.org/10.11002/KJFP.2011.18.3.390
  17. Ebrahimzadeh Attari V, Malek Mahdavi A, Javadivala Z, Mahluji S, Zununi Vahed S, Ostadrahimi A. A systematic review of the anti‐ obesity and weight lowering effect of ginger (Zingiber officinale Roscoe) and its mechanisms of action. Phytother Res. 2018 ; 32(4) : 577-85. https://doi.org/10.1002/ptr.5986
  18. Fuhrman B, Rosenblat M, Hayek T, Coleman R, Aviram M. Ginger extract consumption reduces plasma cholesterol, inhibits LDL oxidation and attenuates development of atherosclerosis in atherosclerotic, apolipoprotein E-deficient mice. J Nutr. 2000 ; 130(5) : 1124-31. https://doi.org/10.1093/jn/130.5.1124
  19. Blois MS, Antioxidant determination by the use of stable free radical. Nature. 1958 ; 191 : 1199-200. https://doi.org/10.1038/1911199a0
  20. Van den Berg R, Haenen GR, Van den Berg H, Bast A. Applicability of an improved trolox equivalent anti-oxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem. 1999 ; 66 : 511-7. https://doi.org/10.1016/S0308-8146(99)00089-8
  21. Wunsch E, Heidrich HG. Quantitativen bestimmung der collagenase. Hoppe-Seyler's Z Physiol Chem. 1963 ; 333(1) : 149-51. https://doi.org/10.1515/bchm2.1963.333.1.149
  22. Floegel A, Kim DO, Chung SJ, Koo SI, Chun O.K. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compost Anal. 2011 ; 24(7) : 1043-8. https://doi.org/10.1016/j.jfca.2011.01.008
  23. Shi J, Gong J, Liu J, Wu X, Zhang Y. Antioxidant capacity of extract from edible flowers of Prunus mume in China and its active components. LWT-Food Sci Technol. 2009 ; 42 : 477-82. https://doi.org/10.1016/j.lwt.2008.09.008
  24. Awika JM, Rooney LW, Wu X, Prior RL, Cineros-Zevallos L. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J Agric Food Chem. 2003 ; 51 : 6657-62. https://doi.org/10.1021/jf034790i
  25. Fellegrini N, Roberta K, Yang M, Rice-Evans C. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation de-colorization assay. Method Enzymol. 1999 ; 299 : 379-89. https://doi.org/10.1016/S0076-6879(99)99037-7
  26. Guon TE, Chung SH. Effect of Zingiber officinale Roscoe extract on antioxidant and apoptosis in A2058 human melanoma cells. J East Asian Soc Diet Life. 2016 ; 26(3) : 207-14. https://doi.org/10.17495/easdl.2016.6.26.3.207
  27. Kumari JA, Venkateshwarlu G, Choukse MK, Anandan R. Effect of essential oil and aqueous extract of ginger (Zingiber officinale) onoxidative stability of fish oil-in-water emulsion. J Food Process Technol. 2014 ; 6 : 412-6.
  28. Lee YM, Bae JH, Jung HY, Kim JH, Park DS. Antioxidant activity in water and methanol extracts from Korean edible wild plants. J Kor Soc Food Sci Nutr. 2011 ; 40(1) : 29-36. https://doi.org/10.3746/JKFN.2011.40.1.029
  29. Shirin AP, Prakash J. Chemical composition and antioxidant properties of ginger root (Zingiber officinale). J Med Plants Res. 2010 ; 24(4) : 2674-9. https://doi.org/10.5897/JMPR09.464
  30. Kim MJ, Park EJ. Feature analysis of different in vitro antioxidant capacity assays and their application to fruit and vegetable samples. J Kor Soc Food Sci Nutr. 2011 ; 40(7) : 1053-62. https://doi.org/10.3746/JKFN.2011.40.7.1053
  31. Jung YS, Park SJ, Park JH, Jhee KH, Lee IS, Yang SA. Effects of ethanol extracts from Zingiber officinale Rosc., Curcuma longa L., and Curcuma aromatica Salisb. on acetylcholinesterase and antioxidant activities as well as GABA contents. Kor Soc Food Sci Nutr. 2012 ; 41(10) : 1395-401. https://doi.org/10.3746/jkfn.2012.41.10.1395
  32. Lee BS, Ko MS, Kim HJ, Kwak IS, Kim DH, Chung BW. Separation of 6-gingerol from ginger [Zingiber officinale Roscoe] and antioxidative activity. KSBB Journal. 2006 ; 21(6) : 484-8.
  33. Ali BH., Blunden G, Tanira MO, Nemmar A. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem Toxicol. 2008 ; 46(2) : 409-20. https://doi.org/10.1016/j.fct.2007.09.085
  34. Slominski A, Tobin DJ, Shibahara S, Wortsman J. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol Rev. 2004 ; 84(4) : 1155-228. https://doi.org/10.1152/physrev.00044.2003
  35. Iwata M, Corn T, Iwata S, Everett MA, Fuller BB. The relationship between tyrosinase activity and skin color in human foreskins. J Investig Dermatol. 1990 ; 95(1) : 9-15. https://doi.org/10.1111/1523-1747.ep12872677
  36. Lee SM. A study on volatile flavor component analysis of ginger essential oil, and its whitening and antibacterial effects. KSCC. 2019 ; 9(3) : 429-39.
  37. Park KJ, Park SH, Kim JK. Anti-wrinkle activity of Acanthopanax senticosus extract in Ultraviolet B (UVB)-induced photoaging. J Kor Soc Food Sci Nutr. 2010 ; 39 : 42-6. https://doi.org/10.3746/JKFN.2010.39.1.042
  38. Oishi Y, Fu ZW, Ohnuki Y, Kato H, Noguchi T. Molecular basis of the alteration in skin collagen metabolism in response to in vivo dexamethasone treatment: effects on the synthesis of collagen type I and III, collagenase, and tissue inhibitors of metalloproteinases. Br J Dermatol. 2002 ; 147(5) : 859-68. https://doi.org/10.1046/j.1365-2133.2002.04949.x
  39. Yoon Y, Bae S, An S, Choe Y, Ahn K, An I. Effects of ultraviolet radiation on the skin and skin cell signaling pathways. Kor J Aesthet Cosmetol. 2013 ; 11 : 417-26.
  40. Abdel-Naeem HH, Mohamed HM. Improving the physico-chemical and sensory characteristics of camel meat burger patties using ginger extract and papain. Meat Science. 2016 ; 118 : 52-60. https://doi.org/10.1016/j.meatsci.2016.03.021
  41. Rodriguez D, Silvera R, Carrio R, Nadji M, Caso R, Rodriguez G, Charyulu V, Torroella-Kouri M. Tumor microenvironment profoundly modifies functional status of macrophages: peritoneal and tumor-associated macrophages are two very different subpopulations. Cell Immunol. 2013 ; 283 : 51-60. https://doi.org/10.1016/j.cellimm.2013.06.008
  42. Guzik T, Korbut R, Adamek-Guzik T. Nitric oxide and superoxide in inflammation. J physiol pharmacol. 2003 ; 54(4) : 469-87.
  43. Farias-Eisner R, Sherman MP, Aeberhard E, Chaudhuri G. Nitric oxide is an important mediator for tumoricidal activity in vivo. Proc Natl Acad Sci USA. 1994 ; 91 : 9407-11. https://doi.org/10.1073/pnas.91.20.9407
  44. Caban M, Chojnacka K, Owczarek K, Laskowska J, Fichna J, Podsedek A, Sosnowska D, Lewandowska, U. Spent hops (Humulus lupulus L.) extract as modulator of the inflammatory response in lipopolysaccharide stimulated raw 264.7 macrophages. J Physiol Pharmacol. 2020 ; 71(1) : 67-78.
  45. Mustafa I, Chin NL, Fakurazi S, Palanisamy A. Comparison of phytochemicals, antioxidant and anti-inflammatory properties of sun-, oven-and freeze-dried ginger extracts. Foods. 2019 ; 8(10) : 1-11.
  46. Oh HK. Antioxidant and anti-inflammatory activities of extracts from Eugenia caryophyllata Thunb. J Korean Soc Food Cult. 2016 ; 31(5) : 481-8 https://doi.org/10.7318/KJFC/2016.31.5.481
  47. Kim JJ, An SI, Lee JS, Yun SM, Lee MY, Yun HS. Suppression of the expression of cyclooxygenase-2 induced by Toll-like receptor 2, 3, and 4 agonists by 6-shogaol. Kor J Food Sci Technol. 2008 ; 40(3) : 332-6.
  48. Lee NK, Ku JE, Han HS, Lee NK, Ku JE, Han HS. Cytoprotective and anti-inflammatory effects of 6-shogaol on human dermal fibroblasts. Asian J Beauty Cosmetol . 2017 ; 15(3) : 367-76. https://doi.org/10.20402/ajbc.2016.0158
  49. Ha SK, Moon E, Ju MS, Kim DH, Ryu JH, Oh MS, Kim SY. 6-Shogaol, a ginger product, modulates neuroinflammation: a new approach to neuroprotection. Neuropharmacology. 2012 ; 63(2) : 211-23. https://doi.org/10.1016/j.neuropharm.2012.03.016