한국환경농학회지 (Korean Journal of Environmental Agriculture)

  • 제40권4호
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  • Pages.248-259
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  • 2021
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  • 1225-3537(pISSN)
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  • 2233-4173(eISSN)
한국환경농학회 (The Korean Society of Environmental Agriculture)
권호 표지
DOI QR코드

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농업용수의 미생물학적 안전성 조사 및 위생지표세균 농도와 병원성미생물 검출률과의 상관관계 분석

Investigation of Microbial Safety and Correlations Between the Level of Sanitary Indicator Bacteria and the Detection Ratio of Pathogens in Agricultural Water

  • 황인준 (농촌진흥청 국립농업과학원 유해생물과) ;
  • 이태권 (연세대학교 환경공학과) ;
  • 박대수 (농촌진흥청 국립농업과학원 유해생물과) ;
  • 김은선 (농촌진흥청 국립농업과학원 유해생물과) ;
  • 최송이 (농촌진흥청 국립농업과학원 유해생물과) ;
  • 현정은 (농촌진흥청 국립농업과학원 유해생물과) ;
  • 나겐드란 라자린감 (농촌진흥청 국립농업과학원 유해생물과) ;
  • 김세리 (농촌진흥청 국립농업과학원 유해생물과) ;
  • 조민 (전북대학교 생명공학부)
  • Hwang, Injun (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Tae Kwon (Department of Environment Engineering, Yonsei University) ;
  • Park, Daesoo (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Eunsun (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Choi, Song-Yi (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hyun, Jeong-Eun (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Rajalingam, Nagendran (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Se-Ri (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Cho, Min (Division of Biotechnology, Chonbuk National University)
  • 투고 : 2021.10.14
  • 심사 : 2021.10.26
  • 발행 : 2021.12.31
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초록

BACKGROUND: Contaminated water was a major source of food-borne pathogens in various recent fresh produce-related outbreaks. This study was conducted to investigate the microbial contamination level and correlations between the level of sanitary indicator bacteria and the detection ratio of pathogens in agricultural water by logistic regression analysis. METHODS AND RESULTS: Agricultural water was collected from 457 sites including surface water (n=300 sites) and groundwater (n=157 sites) in South Korea from 2018 to 2020. Sanitary indicator bacteria (total coliform, fecal coliform, and Escherichia coli) and food-borne pathogens (pathogenic E. coli, E. coli O157:H7, Salmonella spp., and Listeria monocytogenes) were analyzed. In surface water, the coliform, fecal coliform, and E. coli were 3.27±0.89 log CFU/100 mL, 1.90±1.19 log CFU/100 mL, and 1.39±1.26 log CFU/100 mL, respectively. For groundwater, three kinds of sanitary indicators ranged in the level from 0.09 - 0.57 log CFU/100 mL. Pathogenic E. coli, Salmonella and Listeria monocytogenes were detected from 3%-site, 1.5%- site, and 0.6%-site water samples, respectively. According to the results of correlations between the level of sanitary indicator bacteria and the detection ratio of pathogens by logistic regression analysis, the probability of pathogen detection increased individually by 1.45 and 1.34 times as each total coliform and E. coli concentration increased by 1 log CFU/100mL. The accuracy of the model was 70.4%, and sensitivity and specificity were 81.5% and 51.7%, respectively. CONCLUSION(S): The results indicate the need to manage the microbial risk of agricultural water to enhance the safety of fresh produce. In addition, logistic regression analysis is useful to analyze the correlation between the level of sanitary indicator bacteria and the detection ratio of pathogens in agricultural water.

키워드

과제정보

This study was carried out with the support of "Research Program for Agricultural Science & Technology Development (Project No. PJ01419401), National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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