대한한의학방제학회지 (Herbal Formula Science)

  • 제29권1호
  • /
  • Pages.45-55
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  • 2021
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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Dexamethasone으로 유도한 근위축 세포모델에서 흑효모 배양물 유래 polycan의 근위축 개선에 대한 효과

Effects of polysaccharide (polycan) derived from black yeast in dexamethasone-induced muscle atrophy cell model

  • Hwang, Su-Jin (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Lim, Jong-Min (Glucan Co. Ltd.) ;
  • Ku, Bon-Hwa (Glucan Co. Ltd.) ;
  • Cheon, Da-Mi (Glucan Co. Ltd.) ;
  • Jung, Yu Jin (Research Center for Advanced Specialty Chemicals, Korea Research Institute of Chemical Technology (KRICT)) ;
  • Kim, Young-Suk (Glucan Co. Ltd.) ;
  • Oh, Tae Woo (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • 투고 : 2021.02.08
  • 심사 : 2021.02.21
  • 발행 : 2021.02.28
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초록

Objectives : This study was conducted to evaluate the anti-atrophic effect of polycan in dexamethasone-induced skeletal muscle atrophy in vitro model. Methods : C2C12 myoblast were differentiated into myotube by 2% horese serum medium for 6 days, and then treated polycan extract at different concentrations for 24h. The effect of dexamethasone on the induction of muscle atrophy and expression of atrophy-related genes in differentiated C2C12 myotubes using a GSH, ROS, real-time PCR, western blots analysis. Results : The results showed that Treatment with polycan (100 and 200 ㎍/㎖) noncytotoxic levels on both myoblast and myotube. Polycan decreased the ROS level overproduced with dexamethasone and improved the depletion of GSH level. Dexamethasone showed a decrease in myotube diameter, which was associated with up-regulation muscle-specific ubiquitin ligases markers, such as atrogin-1, FoxO3, myostatin and muscle RING finger-1 (MuRF1), and down-regulation of myogenin, MEF2, Myogenic regulatory factor 5, 6 and MyoD. The results showed that polycan treatment significantly dose-dependently inhibited it. Furthermore, decreased expressions of PI3K/Akt signal pathway by dexamethasone were reversed by treatment with polycan. Conclusions : Thus, polycan suppresses dexamethasone induced muscle atrophy in C2C12 myotube in vitro model through activation of PI3K/Akt pathway and protective effect of improve skeletal muscle function.

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참고문헌

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