대한한의학방제학회지 (Herbal Formula Science)

  • 제29권1호
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  • Pages.1-8
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  • 2021
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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DOI QR코드

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Hydrogen peroxide에 의해 유도된 남성 생식 세포 GC-1 cell에 미치는 오자연종환(五子衍宗丸)의 효과 연구

Study of Ojayeonjonghwan on hydrogen peroxide-induced oxidative stress in male reproductive GC-1 germ cell lines

  • 장문석 (경희대학교 한의과대학 처방제형학교실) ;
  • 이호철 (경희대학교 대학원 기초한의과학과) ;
  • 이승호 (경희대학교 대학원 기초한의과학과) ;
  • 박성규 (경희대학교 한의과대학 처방제형학교실)
  • Chang, Mun Seog (Department of Prescriptionology, College of Korean Medicine, Kyung Hee University) ;
  • Lee, Ho Chul (Department of Science in Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Lee, Seung Ho (Department of Science in Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Park, Seong Kyu (Department of Prescriptionology, College of Korean Medicine, Kyung Hee University)
  • 투고 : 2020.11.28
  • 심사 : 2020.12.03
  • 발행 : 2021.02.28
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초록

Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Ojayeonjonghwan (OYH) in GC-1 germ cell lines. Methods : DPPH radical scavenging activity and cell viability assays in GC-1 germ cell lines were performed. In addition, the protective effects of OYH against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines were examined by measuring cell viability after H2O2 treatmet. The formation of ROS and the antioxidant enzymes activity such as SOD and catalase were measured in the same condition. Results : OYH scavenged DPPH radical dose-dependent manner and the IC50 was 63.79 ㎍/ml. OYH showed no cytotoxicity at concentration of 1, 10, 100 ㎍/ml. The hydrogen peroxide-induced cytotoxicity of GC-1 germ cell lines was protected to 53.66% by OYH at concentration of 10 ㎍/ml. OYH effectively inhibited ROS production in GC-1 germ cell lines. Mn SOD and catalase protein expression were significantly increased in GC-1 germ cell lines, but Cu/Zn SOD protein expression was not significantly changed. Conclusions : In conclusion, OYH has antioxidant activities against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines.

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