DOI QR코드

DOI QR Code

Characterization of Xanthomonas citri pv. glycines Population Genetics and Virulence in a National Survey of Bacterial Pustule Disease in Korea

  • Kang, In-Jeong (Division of Crop Cultivation and Environment Research, National Institute of Crop Science) ;
  • Kim, Kyung Seok (Department of Natural Resource Ecology and Management, Iowa State University) ;
  • Beattie, Gwyn A. (Department of Plant Pathology and Microbiology, Iowa State University) ;
  • Chung, Hyunjung (Division of Crop Cultivation and Environment Research, National Institute of Crop Science) ;
  • Heu, Sunggi (Department of Plant Science, Seoul National University) ;
  • Hwang, Ingyu (Department of Agricultural Biotechnology, Seoul National University)
  • 투고 : 2021.11.04
  • 심사 : 2021.11.16
  • 발행 : 2021.12.01

초록

Xanthomonas citri pv. glycines (Xcg) is a major pathogen of soybean (Glycine max) in South Korea, despite the availability of soybean varieties with some resistance. We conducted a nationwide survey of the incidence and severity of bacterial pustule caused by Xcg. The percentage of infected fields was 7% to 17% between 2015 and 2017. We characterized the diversity of a nationwide collection of 106 Xcg isolates based on avrBs3 banding patterns. The isolates fell into 11 groups, each represented by a type strain; only two of these were similar to isolates collected from 1999 to 2002. The diversity of Xcg strains increased and the dominant strains changed between 1999 and 2017, with three new type strains comprising 44% of the isolates examined in 2012 to 2017. Pathogenicity tests did not show evidence for a shift in the races or aggressiveness of Xcg strains. Korean soybean cultivars, including the widely-grown Daewon cultivar, were susceptible to the 11 new type strains. The cultivar CNS, which carries the rxp resistance gene, was susceptible to most type strains, including two representing 83% of the Korean Xcg strains. In contrast, Williams 82, which also carries rxp, showed resistance to at least five type strains. Collectively, these results suggest that Williams 82 has resistance loci in addition to rxp. The widespread distribution of Xcg, the high virulence of the current endemic strains, and the low resistance of most Korean soybean cultivars collectively favor widespread disease in Korea in years that are favorable to pustule development.

키워드

과제정보

This research was co-funded by research grants from the Cooperative Research Program for Ag-riculture Science & Technology Development of the Rural Development Administration in the Republic of Korea (Project title: Identification of distribution and pathogenic variation in isolates of Xanthomonas citri pv. glycines), grant number PJ01357303; the Next-Generation BioGreen 21 Program of the Rural Development Administration in the Republic of Korea (Project title: Development of a platform for breeding of disease resistance in soybean: Bacterial leaf pustule and Phytophthora root rot); grant number PJ01574401.

참고문헌

  1. Athinuwat, D., Prathuangwong, S., Cursino, L. and Burr, T. 2009. Xanthomonas axonopodis pv. glycines soybean cultivar virulence specificity is determined by avrBs3 homolog avrXg1. Phytopathology 99:996-1004. https://doi.org/10.1094/PHYTO-99-8-0996
  2. Bernard, R. L. and Weiss, M. G. 1973. Qualitative genetics. In: Soybeans: improvement, production, and uses, ed. by B. E. Caldwell, pp. 117-164. American Society of Agronomy, Madison, WI, USA.
  3. Bradbury, J. F. 1984. Genus II. Xanthomonas Dowson 1939, 187. In: Bergey's manual of systematic bacteriology, Vol. 1, eds. by N. R. Krieg and J. G. Holt, pp. 199-210. Williams & Wilkins, Baltimore, MD, USA.
  4. Constantin, E. C., Cleenwerck, I., Maes, M., Baeyen, S., Van Malderghem, C., De Vos, P. and Cottyn, B. 2016. Genetic characterization of strains named as Xanthomonas axonopodis pv. dieffenbachiae leads to a taxonomic revision of the X. axonopodis species complex. Plant Pathol. 65:792-806. https://doi.org/10.1111/ppa.12461
  5. Hartman, G. L., Rupe, J. C., Sikora, E. J., Domier, L. L., Davis, J. A. and Steffey, K. L. 2016. Compendium of soybean diseases and pests. 5th ed. American Phytopathological Society, St. Paul, MN, USA.
  6. Hartwig, E. E. and Lehman, S. G. 1951. Inheritance of resistance to bacterial pustule disease in soybeans. Agron. J. 43:226-229. https://doi.org/10.2134/agronj1951.00021962004300050005x
  7. Hokawat, S. and Rudolph, K. 1991. Variation in pathogenicity and virulence of strains of Xanthomonas campestris pv. glycines, the incitant of bacterial pustule of soybean. J. Phytopathol. 131:73-83. https://doi.org/10.1111/j.1439-0434.1991.tb04573.x
  8. Hong, S.-J., Kim, Y.-K., Jee, H.-J., Lee, B.-C., Yoon, Y.-N. and Park, S.-T. 2010. Selection of bactericides for controlling soybean bacterial pustule. Res. Plant Dis. 16:266-273. https://doi.org/10.5423/RPD.2010.16.3.266
  9. Hong, S.-J., Kim, Y.-K., Jee, H.-J., Shim, C.-K., Kim, M.-J., Park, J.-H., Han, E.-J. and Lee, B.-C. 2011. Influence of disease severity of bacterial pustule caused by Xanthomonas axonopodis pv. glycines on soybean yield. Res. Plant Dis. 17:317-325. https://doi.org/10.5423/RPD.2011.17.3.317
  10. Hueck, C. J. 1998. Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol. Mol. Biol. Rev. 62:379-433. https://doi.org/10.1128/mmbr.62.2.379-433.1998
  11. Hwang, I. and Lim, S. M. 1998. Pathogenic variability in isolates of Xanthomonas campestris pv. glycines. Korean J. Plant Pathol. 14:19-22.
  12. Kaewnum, S., Prathuangwong, S. and Burr, T. J. 2005. Aggressiveness of Xanthomonas axonopodis pv. glycines isolates to soybean and hypersensitivity responses by other plants. Plant Pathol. 54:409-415. https://doi.org/10.1111/j.1365-3059.2005.01176.x
  13. Kim, J.-G., Choi, S., Oh, J., Moon, J. S. and Hwang, I. 2006. Comparative analysis of three indigenous plasmids from Xanthomonas axonopodis pv. glycines. Plasmid 56:79-87. https://doi.org/10.1016/j.plasmid.2006.03.001
  14. Lee, J. and Shin, H. 2021. Assessment of future climate change impact on an agricultural reservoir in South Korea. Water 13:2125. https://doi.org/10.3390/w13152125
  15. Lee, S. D. 1999. Occurrence and characterization of major plant bacterial diseases in Korea. Ph.D. thesis. Seoul National University, Seoul, Korea.
  16. Moffett, M. L. and Croft, B. J. 1983. Xanthomonas. In: Plant bacterial diseases: a diagnostic guide, eds. by P. C. Fahy and G. J. Persley, pp. 189-228. Academic Press, New York, NY, USA.
  17. Narvel, J. M., Jakkula, L. R., Phillips, D. V., Wang, T., Lee, S.-H. and Boerma, H. R. 2001. Molecular mapping of Rxp conditioning reaction to bacterial pustule in soybean. J. Hered. 92:267-270. https://doi.org/10.1093/jhered/92.3.267
  18. Park, H.-J., Han, S.-W., Oh, C., Lee, S., Ra, D., Lee, S.-H. and Heu, S. 2008. Avirulence gene diversity of Xanthomonas axonopodis pv. glycines isolated in Korea. J. Microbiol. Biotechnol. 18:1500-1509.
  19. Rural Development Administration. 2012. The agricultural science and technology research and analysis standards. 5th ed. Rural Development Administration, Suwon, Korea. 424 pp.
  20. Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA. 1546 pp.
  21. Shukla, A. K. 1994. Pilot estimation studies of soybean (Glycine max) yield losses by various levels of bacterial pustule (Xanthomonas campestris pv. glycines) infection. Int. J. Pest Manage. 40:249-251. https://doi.org/10.1080/09670879409371892