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Anti-inflammatory Effect and Mechanism of Citri Reticulatae Viride Pericarpium Water Extract

청피 물 추출물의 항염증 효과와 기전 연구

  • Ahn, Tae-Seok (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University) ;
  • Hwang, Deok-Sang (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University) ;
  • Lee, Jin-Moo (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University) ;
  • Jang, Jun-Bock (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University) ;
  • Lee, Chang-Hoon (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
  • 안태석 (경희대학교 대학원 임상한의학과) ;
  • 황덕상 (경희대학교 대학원 임상한의학과) ;
  • 이진무 (경희대학교 대학원 임상한의학과) ;
  • 장준복 (경희대학교 대학원 임상한의학과) ;
  • 이창훈 (경희대학교 대학원 임상한의학과)
  • Received : 2021.01.10
  • Accepted : 2021.02.26
  • Published : 2021.02.26

Abstract

Objectives: This study was designed to examine anti-inflammatory effect and mechanism of Citri Reticulatae Viride Pericarpium water extract (CRE). Methods: Cell cytotoxicity was tested with RAW 264.7 cells. To investigate anti-inflammatory effect of CRE in lipopolysaccharide (LPS)-induced RAW 264.7 cell, we measured nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10). In addition, mitrogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) were examined by western blotting in LPS-induced RAW 264.7 cell with treated CRE. Results: In cytotoxicity analysis, CRE does not affect cell cytotoxicity. As compared with the control group, the expression of NO, PGE2, TNF-α, IL-6 were significantly decreased, and IL-10 was significantly increased in LPS-induced RAW 264.7 cell with treated CRE. As a result of Western blotting, there was concentration-dependent inhibition of pp38, pERK in MAPK pathway and significant reduction of pp65 in the NF-κB pathway. Conclusions: CRE might have anti-inflammatory effect in LPS-induced macrophages by promoting the production of IL-10.

Keywords

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