Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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p-coumaric acid, an active ingredient of Panax ginseng, ameliolates atopic dermatitis-like skin lesions through inhibition of thymic stromal lymphopoietin in mice

  • Moon, Phil-Dong (Center for Converging Humanities, Kyung Hee University) ;
  • Han, Na-Ra (Department of Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Lee, Jin Soo (Department of Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Hyung-Min (Department of Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Jeong, Hyun-Ja (Division of Food and Pharmaceutical Engineering, BioChip Research Center, Hoseo University)
  • Received : 2020.03.25
  • Accepted : 2020.06.28
  • Published : 2021.01.15
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Abstract

Background: Atopic dermatitis (AD) is associated with chronic skin inflammatory reactions. p-coumaric acid (pCA) is an active ingredient of Panax ginseng Meyer (Araliaceae). Methods: Here, we estimated an anti-AD effect of pCA on activated mast cells, activated splenocytes, and a mouse model of AD. Cytokines levels were measured by ELISA and protein activation was analyzed by Western blotting. 2,4-dinitrofluorobenzene (DNFB) was used to induce AD-like skin lesions. Results: The treatment with pCA suppressed the productions and mRNA expressions of thymic stromal lymphopoietin (TSLP), TNF-α, IL-6, and IL-1β in HMC-1 cells. pCA downregulated the expressions of RIP2 and caspase-1, phosphorylated-(p)p38/pJNK/pERK, and pIKKβ/pIkBα/NF-κB in HMC-1 cells. pCA also decreased the productions of TSLP, TNF-α, IL-6, IL-4, and IFN-γ in the supernatant of stimulated splenic cells. Comparing to DNFB-sensitized control group, pCA-treated group alleviated pathological changes of AD-like lesions. pCA decreased the proteins and mRNA expressions levels of TSLP, IL-6, and IL-4 in the skin lesions. Caspase-1 activation was also downregulated by pCA treatment in the AD-like lesions. The serum levels of histamine, IgE, TSLP, TNF-α, IL-6, and IL-4 were suppressed following treatment with pCA. Conclusion: This study suggests that pCA has the potential to improve AD by suppressing TSLP as well as inflammatory cytokines via blocking of caspase-1/NF-κB signal cascade.

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