Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Purification of ginseng rare sapogenins 25-OH-PPT and its hypoglycemic, antiinflammatory and lipid-lowering mechanisms

  • Xu, Jing (School of Functional Food and Wine, Shenyang Pharmaceutical University) ;
  • Liu, Hairong (School of Functional Food and Wine, Shenyang Pharmaceutical University) ;
  • Su, Guangyue (School of Functional Food and Wine, Shenyang Pharmaceutical University) ;
  • Ding, Meng (School of Functional Food and Wine, Shenyang Pharmaceutical University) ;
  • Wang, Wei (School of Functional Food and Wine, Shenyang Pharmaceutical University) ;
  • Lu, Jincai (Department of Medicinal Plant Shenyang Pharmaceutical University) ;
  • Bi, Xiuli (School of Life Science, Liaoning University) ;
  • Zhao, Yuqing (School of Functional Food and Wine, Shenyang Pharmaceutical University)
  • Received : 2018.09.13
  • Accepted : 2019.11.01
  • Published : 2021.01.15
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Abstract

Background: Panax ginseng Meyer has been used as a nourishing edible herb in East Asia for thousands of years. 25-OH-PPT was first discovered as a natural rare triterpenoid saponin in ginseng stems and leaves by our group. Research found that it showed strong inhibitory effects on α-glucosidase and protein tyrosine phosphatase 1B, and protected cardiocytes (H9c2) through PI3K/Akt pathway. Methods: In the research, in order to optimize the 25-OH-PPT enrichment process, optimal macroporous resins and optimal purification conditions were studied. Meanwhile, the hypoglycemic effect and mechanism of 25-OH-PPT were evaluated by using STZ to establish insulin-dependent diabetic mice and the spontaneous type 2 diabetes DB/DB mice. Results and Conclusion: Research found that 25-OH-PPT can reduce blood glucose and enhance glucose tolerance in STZ model mice. It increases insulin sensitivity by upregulating GLUT4 and AMPK in skeletal muscle, and activating insulin signaling pathways. In DB/DB mice, 25-OH-PPT achieves hypoglycemic effects mainly by activating the insulin signaling pathway. Meanwhile, through the influence of liver inflammatory factors and lipids in serum, it can be seen that 25-OH-PPT has obvious anti-inflammatory and lipid-lowering effects. These results provide new insights into the study of ginseng as a functional food.

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References

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