Animal Bioscience

  • 제34권4호
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  • Pages.670-679
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  • 2021
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Chicken serum uric acid level is regulated by glucose transporter 9

  • Ding, Xuedong (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Peng, Chenglu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Li, Siting (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Li, Manman (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Li, Xinlu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Wang, Zhi (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Li, Yu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Wang, Xichun (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Li, Jinchun (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Wu, Jinjie (College of Animal Science and Technology, Anhui Agricultural University)
  • 투고 : 2020.02.13
  • 심사 : 2020.05.29
  • 발행 : 2021.04.01
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초록

Objective: Glucose transporter 9 (GLUT9) is a uric acid transporter that is associated with uric absorption in mice and humans; but it is unknown whether GLUT9 involves in chicken uric acid regulation. This experiment aimed to investigate the chicken GLUT9 expression and serum uric acid (SUA) level. Methods: Sixty chickens were divided into 4 groups (n = 15): a control group (NC); a sulfonamide-treated group (SD) supplemented with sulfamonomethoxine sodium via drinking water (8 mg/L); a fishmeal group (FM) supplemented with 16% fishmeal in diet; and a uric acid-injection group (IU), where uric acid (250 mg/kg) was intraperitoneally injected once a day. The serum was collected weekly to detect the SUA level. Liver, kidney, jejunum, and ileum tissues were collected to detect the GLUT9 mRNA and protein expression. Results: The results showed in the SD and IU groups, the SUA level increased and GLUT9 expression increased in the liver, but decreased in the kidney, jejunum, and ileum. In the FM group, the SUA level decreased slightly and GLUT9 expression increased in the kidney, but decreased in the liver, jejunum, and ileum. Correlation analysis revealed that liver GLUT9 expression correlated positively, and renal GLUT9 expression correlated negatively with the SUA level. Conclusion: These results demonstrate that there may be a feedback regulation of GLUT9 in the chicken liver and kidney to maintain the SUA balance; however, the underlying mechanism needs to be investigated in future studies.

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참고문헌

  1. Yamauchi T, Ueda T. Primary hyperuricemia due to decreased renal uric acid excretion. Nihon Rinsho 2008;66:679-81.
  2. El Ridi R, Tallima H. Physiological functions and pathogenic potential of uric acid: a review. J Adv Res 2017;8:487-93. https://doi.org/10.1016/j.jare.2017.03.003
  3. Xu L, Shi Y, Zhuang S, Liu N. Recent advances on uric acid transporters. Oncotarget 2017;8:100852-62. https://doi.org/10.18632/oncotarget.20135
  4. Mobasheri A, Dobson H, Mason SL, et al. Expression of the GLUT1 and GLUT9 facilitative glucose transporters in em - bryonic chondroblasts and mature chondrocytes in ovine articular cartilage. Cell Biol Int 2005;29:249-60. https://doi.org/10.1016/j.cellbi.2004.11.024
  5. Vitart V, Rudan I, Hayward C, et al. SLC2A9 is a newly identified urate transporter influencing serum urate concentration, urate excretion and gout. Nat Genet 2008;40:437-42. https://doi.org/10.1038/ng.106
  6. Kimura T, Amonpatumrat S, Tsukada A, et al. Increased expression of SLC2A9 decreases urate excretion from the kidney. Nucleosides Nucleotides Nucleic Acids 2011;30:1295-301. https://doi.org/10.1080/15257770.2011.628354
  7. Ruiz A, Gautschi I, Schild L, Bonny O. Human mutations in SLC2A9 (Glut9) affect transport capacity for urate. Front Physiol 2018;9:476. https://doi.org/10.3389/fphys.2018.00476
  8. Mueckler M, Thorens B. The SLC2 (GLUT) family of membrane transporters. Mol Aspects Med 2013;34:121-38. https://doi.org/10.1016/j.mam.2012.07.001
  9. Clemencon B, Luscher BP, Fine M, et al. Expression, purification, and structural insights for the human uric acid transporter, GLUT9, using the Xenopus laevis oocytes system. PLoS One 2014;9:e108852. https://doi.org/10.1371/journal.pone.0108852
  10. Augustin R, Carayannopoulos MO, Dowd LO, Phay JE, Moley JF, Moley KH. Identification and characterization of human glucose transporter-like protein-9 (GLUT9): alternative splicing alters trafficking. J Biol Chem 2004;279:16229-36. https://doi.org/10.1074/jbc.M312226200
  11. Bu P, Le Y, Zhang Y, Cheng X. Hormonal and chemical regulation of the Glut9 transporter in mice. J Pharmacol Exp Ther 2017;360:206-14. https://doi.org/10.1124/jpet.116.237040
  12. Preitner F, Bonny O, Laverrière A, et al. Glut9 is a major regulator of urate homeostasis and its genetic inactivation induces hyperuricosuria and urate nephropathy. Proc Natl Acad Sci USA 2009;106:15501-6. https://doi.org/10.1073/pnas.0904411106
  13. Matsuo H, Chiba T, Nagamori S, et al. Mutations in glucose transporter 9 gene SLC2A9 cause renal hypouricemia. Am J Hum Genet 2008;83:744-51. https://doi.org/10.1016/j.ajhg.2008.11.001
  14. Bibert S, Hess SK, Firsov D, et al. Mouse GLUT9: evidences for a urate uniporter. Am J Physiol Renal Physiol 2009;297:F612-9. https://doi.org/10.1152/ajprenal.00139.2009
  15. Keebaugh AC, Thomas JW. The evolutionary fate of the genes encoding the purine catabolic enzymes in hominoids, birds, and reptiles. Mol Biol Evol 2010;27:1359-69. https://doi.org/10.1093/molbev/msq022
  16. Auberson M, Stadelmann S, Stoudmann C, et al. SLC2A9 (GLUT9) mediates urate reabsorption in the mouse kidney. Pflugers Arch 2018;470:1739-51. https://doi.org/10.1007/s00424-018-2190-4
  17. Torres RJ, Puig JG. GLUT9 influences uric acid concentration in patients with Lesch-Nyhan disease. Int J Rheum Dis 2018;21:1270-6. https://doi.org/10.1111/1756-185X.13323
  18. Zhang W, Sumners LH, Siegel PB, Cline MA, Gilbert ER. Quantity of glucose transporter and appetite-associated factor mRNA in various tissues after insulin injection in chickens selected for low or high body weight. Physiol Genomics 2013;45:1084-94. https://doi.org/10.1152/physiolgenomics.00102.2013
  19. Bai S, Pan S, Zhang K, et al. Long-term effect of dietary overload lithium on the glucose metabolism in broiler chickens. Environ Toxicol Pharmacol 2017;54:191-8. https://doi.org/10.1016/j.etap.2017.07.011
  20. Guo X, Huang K, Tang J. Clinicopathology of gout in growing layers induced by high calcium and high protein diets. Br Poult Sci 2005;46:641-6. https://doi.org/10.1080/00071660500302661
  21. Mustafa S, Alsughayer A, Elgazzar A, Elassar A, Al Sagheer F. Effect of sulfa drugs on kidney function and renal scintigraphy. Nephrology 2014;19:210-6. https://doi.org/10.1111/nep.12200
  22. Romi MM, Arfian N, Tranggono U, Setyaningsih WAW, Sari DCR. Uric acid causes kidney injury through inducing fibroblast expansion, Endothelin-1 expression, and inflammation. BMC Nephrol 2017;18:326. https://doi.org/10.1186/s12882-017-0736-x
  23. Zhiqiang C. Studies of determination of calcium, phosphonium and uric acid in urine by automatic biochemical analyzer. J Clin Urol 2006;6:461-3. https://doi.org/10.3969/j.issn.1001-1420.2006.06.023
  24. Zeng Y, Callaghan D, Xiong H, Yang Z, Huang P, Zhang W. Abcg2 deficiency augments oxidative stress and cognitive deficits in Tg-SwDI transgenic mice. J Neurochem 2012;122:456-69. https://doi.org/10.1111/j.1471-4159.2012.07783.x
  25. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  26. Smith PK, Krohn RI, Hermanson GT, et al. Measurement of protein using bicinchoninic acid. Anal Biochem 1985;150:76-85. https://doi.org/10.1016/0003-2697(85)90442-7
  27. Francisco JS, de Moraes HP, Dias EP. Evaluation of the Image-Pro Plus 4.5 software for automatic counting of labeled nuclei by PCNA immunohistochemistry. Braz Oral Res 2004;18:100-4. https://doi.org/10.1590/S1806-83242004000200002
  28. Liu Y, Sun W, Zhang X, Li J, Zhang H. Compound tufuling granules regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice. Chin J Integr Med 2015;21:823-9. https://doi.org/10.1007/s11655-015-2052-2
  29. Field A. Discovering statistics using IBM SPSS statistics. 4ed. Thousand Oaks, CA, USA: Sage Publications; 2013.
  30. Ghoodjani A. Advanced statistical methods and applications. Iran: Statistica; 2018.
  31. Maesaka JK, Fishbane S. Regulation of renal urate excretion: a critical review. Am J Kidney Dis 1998;32:917-33. https://doi.org/10.1016/S0272-6386(98)70067-8
  32. Oka Y, Tashiro H, Sirasaki R, et al. Hyperuricemia in hematologic malignancies is caused by an insufficient urinary excretion. Nucleosides Nucleotides Nucleic Acids 2014;33:434-8. https://doi.org/10.1080/15257770.2013.872274
  33. Keembiyehetty C, Augustin R, Carayannopoulos MO, et al. Mouse glucose transporter 9 splice variants are expressed in adult liver and kidney and are up-regulated in diabetes. Mol Endocrinol 2006;20:686-97. https://doi.org/10.1210/me.2005-0010
  34. Anzai N, Ichida K, Jutabha P, et al. Plasma urate level is directly regulated by a voltage-driven urate efflux transporter URATv1 (SLC2A9) in humans. J Biol Chem 2008;283:26834-8. https://doi.org/10.1074/jbc.C800156200
  35. DeBosch BJ, Kluth O, Fujiwara H, Schurmann A, Moley K. Early-onset metabolic syndrome in mice lacking the intestinal uric acid transporter SLC2A9. Nat Commun 2014;5:4642. https://doi.org/10.1038/ncomms5642
  36. Nagura M, Tamura Y, Kumagai T, Hosoyamada M, Uchida S. Uric acid metabolism of kidney and intestine in a rat model of chronic kidney disease. Nucleosides Nucleotides Nucleic Acids 2016;35:550-8. https://doi.org/10.1080/15257770.2016.1163379