Animal Bioscience

  • 제34권4호
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  • Pages.525-532
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  • 2021
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Mitochondrial DNA variation and phylogeography of Old World camels

  • Ming, Liang (Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • Siren, Dalai (Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • Yi, Li (Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • Hai, Le (Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • He, Jing (Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • Ji, Rimutu (Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University)
  • 투고 : 2020.05.09
  • 심사 : 2020.07.23
  • 발행 : 2021.04.01
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초록

Objective: Old World camels are a valuable genetic resource for many countries around the world due to their adaptation to the desert environment. At present, Old World camels have encountered the challenge of unprecedented loss of genetic resources. Through our research, we would reveal the population structure and genetic variation in Old World camel populations, which provides a theoretical basis for understanding the germplasm resources and origin and evolution of different Old World camel populations. Methods: In the present study, we assessed mtDNA control region sequences of 182 individuals from Old World camels to unravel genetic diversity, phylogeography, and demographic dynamics. Results: Thirty-two haplotypes confirmed by 54 polymorphic sites were identified in the 156 sequences, which included 129 domestic and 27 wild Bactrian camels. Meanwhile, 14 haplotypes were defined by 47 polymorphic sites from 26 sequences in the dromedaries. The wild Bactrian camel population showed the lowest haplotype and nucleotide diversity, while the dromedaries investigated had the highest. The phylogenetic analysis suggests that there are several shared haplotypes in different Bactrian camel populations, and that there has been genetic introgression between domestic Bactrian camels and dromedaries. In addition, positive values of Tajima's D and Fu's Fs test demonstrated a decrease in population size and/or balancing selection in the wild Bactrian camel population. In contrast, the negative values of Tajima's D and Fu's Fs test in East Asian Bactrian camel populations explained the demographic expansion and/or positive selection. Conclusion: In summary, we report novel information regarding the genetic diversity, population structure and demographic dynamics of Old World camels. The findings obtained from the present study reveal that abundant genetic diversity occurs in domestic Bactrian camel populations and dromedaries, while there are low levels of haplotype and nucleotide diversity in the wild Bactrian camel population.

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참고문헌

  1. Burger PA. The history of Old World camelids in the light of molecular genetics. Trop Anim Health Prod 2016;48:90513. https://doi.org/10.1007/s11250-016-1032-7
  2. Saipolda T. Mongolian camels. In: Cardellino R, Rosati A, Mosconi C, editors. Proceedings of the current status of genetic resources, recording and production systems in African, Asian and American camelids; 2004 May 30: Sousse, Tunisia. Rome, Italy: ICAR; 2004. pp.73-9.
  3. Burger PA, Ciani E, Faye B. Old World camels in a modern world - a balancing act between conservation and genetic improvement. Anim Genet 2019;50:598-612. https://doi.org/10.1111/age.12858
  4. Hare J. Camelus ferus. The IUCN red list of threatened species. 2008. https://doi.org/10.2305/IUCN.UK.2008.RLTS.T63543A12689285.en
  5. Lei Y, Hare J, Guoying Y, Yun C. The status of the wild camel in China. In: Knoll EM, Burger P, editors. Camels in Asia and North Africa: interdisciplinary perspectives on their past and present significance. Vienna, Austria: Austrian Academy of Sciences Press; 2012. pp. 55-60.
  6. Yadamsuren A, Dulamtseren E, Reading RP. The conservation status and management of wild camels in Mongolia. In: Knoll EM, Burger P, editors. Camels in Asia and North Africa: interdisciplinary perspectives on their past and present significance. Vienna, Austria: Austrian Academy of Sciences Press; 2012. pp.45-54.
  7. Al-Ali AK, Husayni HA, Power DM. A comprehensive biochemical analysis of the blood of the camel (Camelus dromedarius). Comp Biochem Physiol B 1988;89:35-7. https://doi.org/10.1016/0305-0491(88)90257-X
  8. Hamers-Casterman C, Atarhouch T, Muyldermans S, et al. Naturally occurring antibodies devoid of light chains. Nature 1993;363:446-8. https://doi.org/10.1038/363446a0
  9. Wu H, Guang X, Al-Fageeh MB, et al. Camelid genomes reveal evolution and adaptation to desert environments. Nat Commun 2014;5,5188. https://doi.org/10.1038/ncomms6188
  10. Ming L, Wang Z, Yi L, et al. Chromosome-level assembly of wild Bactrian camel genome reveals organization of immune gene loci. Mol Ecol Resour 2020;20:770-80. https://doi.org/10.1111/1755-0998.13141
  11. Ji R, Cui P, Ding F, et al. Monophyletic origin of domestic bactrian camel (Camelus bactrianus) and its evolutionary relationship with the extant wild camel (Camelus bactrianus ferus). Anim Genet 2009;40:377-82. https://doi.org/10.1111/j.1365-2052.2008.01848.x
  12. Mohandesan E, Fitak RR, Corander J, et al. Mitogenome sequencing in the genus Camelus reveals evidence for purifying selection and long-term divergence between wild and domestic Bactrian camels. Sci Rep 2017;7:9970. https://doi.org/10.1038/s41598-017-08995-8
  13. Cui P, Ji R, Ding F, et al. A complete mitochondrial genome sequence of the wild two-humped camel (Camelus bactrianus ferus): an evolutionary history of camelidae. BMC Genomics 2007;8:241. https://doi.org/10.1186/1471-2164-8-241
  14. Almathen F, Charruau P, Mohandesan E, et al. Ancient and modern DNA reveal dynamics of domestication and cross-continental dispersal of the dromedary. Proc Natl Acad Sci USA 2016;113:6707-12. https://doi.org/10.1073/pnas.1519508113
  15. Ming L, Yi L, Sa R, Wang ZX, Wang Z, Ji R. Genetic diversity and phylogeographic structure of Bactrian camels shown by mitochondrial sequence variations. Anim Genet 2017;48:217-20. https://doi.org/10.1111/age.12511
  16. Yi L, Ai Y, Ming L, et al. Molecular diversity and phylogenetic analysis of domestic and wild Bactrian camel populations based on the mitochondrial ATP8 and ATP6 genes. Livest Sci 2017;199:95-100. https://doi.org/10.1016/j.livsci.2017.03.015
  17. Chuluunbat B, Charruau P, Silbermayr K, Khorloojav T, Burger PA. Genetic diversity and population structure of Mongolian domestic Bactrian camels (Camelus Bactrianus). Anim Genet 2014;45:550-8. https://doi.org/10.1111/age.12158
  18. Mate ML, Rocco FD, Zambelli A, Vidal-Rioja L. Mitochondrial DNA structure and organization of the control region of South American camelids. Mol Ecol Notes 2004;4:765-7. https://doi.org/10.1111/j.1471-8286.2004.00744.x
  19. Silbermayr K, Orozco-terWengel P, Charruau P, et al. High mitochondrial differentiation levels between wild and domestic Bactrian camels: a basis for rapid detection of maternal hybridization. Anim Genet 2010;41:315-8. https://doi.org/10.1111/j.1365-2052.2009.01993.x
  20. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  21. Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 2009;25:1451-2. https://doi.org/10.1093/bioinformatics/btp187
  22. Bandelt HJ, Forster P, Rohl A. Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 1999;16:3748. https://doi.org/10.1093/oxfordjournals.molbev.a026036
  23. Rogers AR, Harpending H. Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol 1992;9:552-69. https://doi.org/10.1093/oxfordjournals.molbev.a040727
  24. Harpending HC. Signature of ancient population growth in a low-resolution mitochondrial DNA mismatch distribution. Hum Biol 1994;66:591-600.
  25. Fu YX. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 1997;147:915-25. https://doi.org/10.1093/genetics/147.2.915
  26. Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 1989;123:58595. https://doi:10.1101/gad.3.11.1801
  27. Excoffier L, Lischer HE. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and windows. Mol Ecol Resour 2010;10:564-7. https://doi.org/10.1111/j.1755-0998.2010.02847.x
  28. Al-Jumaili AS, Boudali SF, Kebede A, et al. The maternal origin of indigenous domestic chicken from the Middle East, the north and the horn of Africa. BMC Genetics 2020;21:30. https://doi.org/10.1186/s12863-020-0830-0
  29. Ramos-Onsins SE, Rozas J. Statistical properties of new neutrality tests against population growth. Mol Biol Evol 2002;19:2092-100. https://doi.org/10.1093/oxfordjournals.molbev.a004034
  30. Rogers AR, Harpending H. Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol 1992;9:552-69. https://doi.org/10.1093/oxfordjournals.molbev.a040727
  31. Ming L, Yi L, Guo FC, Siriguleng S, Jirimutu J. Molecular phylogeny of the Bactrian camel based on mitochondrial cytochrome b gene sequences. Genet Mol Res 2016;15:15038983. https://doi.org/10.4238/gmr.15038983
  32. Ming L, Yuan L, Yi L, et al. Whole-genome sequencing of 128 camels across Asia reveals origin and migration of domestic. Commun Biol 2020;3:1. https://doi.org/10.1038/s42003019-0734-6

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