The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Liriope muscari Water Extracts on the Cell Death and Inflammatory Cytokine Expression of Poly I:C-treated Lung Carcinoma Cells

맥문동 열수 추출물이 Poly I:C를 처리한 폐암세포주의 사멸 및 염증성 사이토카인 발현에 미치는 영향

  • Kang, Dayeon (Department of Biochemistry, Chungnam National University) ;
  • Cho, Namjoon (Department of Biochemistry, Chungnam National University) ;
  • Renchinkhand, Gereltuya (Division of Animal Resources Science, Chungnam National University) ;
  • Lee, Bo-Hee (Gogi Berry Research Institute, Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Kim, Eun-Mi (Department of Predictive Toxicology, Korea Institute of Toxicology) ;
  • Nam, Myoung Soo (Division of Animal Resources Science, Chungnam National University) ;
  • Kim, Keekwang (Department of Biochemistry, Chungnam National University)
  • 강다연 (충남대학교 생화학과) ;
  • 조남준 (충남대학교 생화학과) ;
  • 렌친핸드 (충남대학교 동물자원과학부) ;
  • 이보희 (충남농업기술원 구기자연구소) ;
  • 김은미 (안전성평가연구소 예측독성연구본부) ;
  • 남명수 (충남대학교 동물자원과학부) ;
  • 김기광 (충남대학교 생화학과)
  • Received : 2020.12.03
  • Accepted : 2021.01.25
  • Published : 2021.01.30
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Abstract

Objectives : Virus infection through the respiratory tract causes various inflammatory diseases such as pneumonia, cystic fibrosis, and obstructive pulmonary disease, causing enormous social damage. Therefore, it is very important to develop a treatment and prevention of infectious diseases. In this study, we investigated the effect of water extracts of Liriope muscari (WELM), known to improve lung function, on the inflammatory response of lung carcinoma cell line A549 cells induced by the viral double stranded RNA mimetic Polyinosinic:polycytidylic acid (Poly I:C). Methods : The cell viability by WELM treatment was analyzed using MTS assay in A549 cells. After inducing an inflammatory response to WELM-treated A549 cells with Poly I:C, the degree of apoptosis was confirmed through bright field microscopy. Interferon beta (IFN-β) mRNA expression level in A549 cells was analyzed by quantitative reverse transcription PCR (qRT-PCR). Results : WELM treatment has no significant effect on cell viability of A549 cells. We confirmed that pre-treatment of WELM effectively reduces the Poly I:C-induced apoptotic cell death in A549 cells. In addition, it was confirmed that the mRNA expression level of IFN-β, a pro-inflammatory cytokine increased by Poly I:C treatment, was significantly suppressed by WELM treatment in A549 cells. Conclusions : These results provide the evidence that WELM is effective at inhibiting inflammation on respiratory viral infections and suggest that Liriope muscari might be a valuable natural substance in the prevention and treatment of infectious diseases.

Keywords

References

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