The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Evaluation on antioxidative and antiaging effect of immature fruits from Citrus unshiu Markovich

미숙감귤의 항산화 및 항노화 활성에 대한 평가

  • Choi, Moon-Yeol (Department of Herbal Pharmcology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center) ;
  • Kim, Jae Soo (Department of Acupuncture & Moxibustion medicine, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmcology, College of Korean Medicine, Daegu Haany University)
  • 최문열 (구한의대학교 한의과대학 본초약리학교실) ;
  • 박해진 (DHU 바이오융복합시험센터) ;
  • 김재수 (대구한의대학교 한의과대학 침구의학교실) ;
  • 김미려 (구한의대학교 한의과대학 본초약리학교실)
  • Received : 2020.12.11
  • Accepted : 2021.01.25
  • Published : 2021.01.30
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Abstract

Objective : This study was designed to investigate effect of immature fruits from Citrus unshiu Markovich to mature them on antioxidative and antiaging-related enzyme activities in vitro. Methods : Fruits from Open field-cultivated C. unshiu Markovich (ripe and unripe fruits of Citri) made a purchase in Jeju island. We measured total polyphenol and flavonoid contents in mature C. unshiu Markovich (MC) and immature C. unshiu Markovich (IMC) 70% ethanol extract. DPPH free radical, ABTS radical, and hydroxyl radical scavenging activities in both extracts were determined. Also, reducing power, SOD-like activities, elastase inhibition, and collagenase inhibition activities of these extract were assayed at 5, 1, 0.5, and 0.1 mg/㎖. Results : The total polyphenol contents in the ethanol extract of IMC were higher than those of MC (26.99 ± 0.89 mg/TAEg). And total flavonoid contents in them were very similar to both extract (MC; 7.25 ± 0.4 mg/RUEg and IMC; 7.44 ± 0.18 mg/RUEg). DPPH free radical scavenging activities, ABTS radical scavenging capacity, reducing power, and SOD-like activities of IMC-treated group showed significant increase compared to those of MC group in all treated concentrations. Particularly, DPPH free radical scavenging and elastase inhibition activities in IMC-treated group at 5 mg/㎖ showed similar with positive control group. Besides, IMC extract at 5 mg/㎖ concentration surpassed positive control (BHA or Vit. C) in SOD-like activities and ABTS radical scavenging capacity. Conclusion : These results suggest that IMC ethanol extract may be used as a natural antioxidant and a antiaging material for development of functional foods and cosmeceuticals.

Keywords

References

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