Animal Bioscience

  • 제34권1호
  • /
  • Pages.93-101
  • /
  • 2021
  • /
  • 2765-0189(pISSN)
  • /
  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Correlation analysis of muscle amino acid deposition and gut microbiota profile of broilers reared at different ambient temperatures

  • Yang, Yuting (Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University) ;
  • Gao, Huan (Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University) ;
  • Li, Xing (Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University) ;
  • Cao, Zhenhui (Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University) ;
  • Li, Meiquan (Department of Animal Husbandry and Veterinary Medicine, College of Agriculture, Kunming University) ;
  • Liu, Jianping (Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University) ;
  • Qiao, Yingying (Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University) ;
  • Ma, Li (Yunnan Vocational and Technical College of Agriculture) ;
  • Zhao, Zhiyong (Yunnan Animal Science and Veterinary Institute) ;
  • Pan, Hongbin (Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University)
  • 투고 : 2020.05.08
  • 심사 : 2020.08.10
  • 발행 : 2021.01.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: Temperature could influence protein and amino acid deposition as well as gut microbiota profile and composition. However, the specific effects of ambient temperature on amino acids deposition and gut microbiota composition remain insufficiently understood. Methods: A total of 300 one-day-old Avian broilers were randomly divided into three groups and reared at high, medium, and low temperature (HT, MT, and LT), respectively. Breast muscle and fecal samples were collected for amino acid composition analysis and 16S rRNA gene sequence analysis. Results: Our data showed that compared to the MT group, there was a decrease of muscle leucine and tyrosine (p<0.05), as well as an increase of methionine in the HT group (p<0.05) and a decrease of serine in the LT group. Examination of microbiota shift revealed that at genus level, the relative abundance of Turicibacter and Parabacteroides was increased in the HT group (p<0.05) and that the relative abundances of Pandoraea, Achromobacter, Prevotella, Brevundimonas, and Stenotrophomonas in the LT group were higher than those in the MT group (p<0.05). In addition, there were substantial correlations between microbes and amino acids. In the HT group. Turicibacter was negatively correlated with aspartic acid and tyrosine, whereas Parabacteroides was positively correlated with methionine (p<0.05). In the LT group, there were multiple positive correlations between Achromobacter and arginine, isoleucine or tyrosine; between Prevotella and cysteine or phenylalanine; between Brevundimonas and cysteine; and between Stenotrophomonas and cysteine as well as a negative correlation between Stenotrophomonas and serine. Conclusion: Our findings demonstrated that amino acid content of breast muscle and intestinal microbiota profile was affected by different ambient temperatures. Under heat exposure, augmented abundance of Parabacteroides was correlated with elevated methionine. Low temperature treatment may affect muscle tyrosine content through the regulation of Achromobacter.

키워드

과제정보

The authors want to thank Qiaoping Ji for providing the poultry feed. We thank Qihua Li for experimental equipment. This study was supported by the National Key R&D Program of China (2016YFD0500501).

참고문헌

  1. Lara LJ, Rostagno MH. Impact of heat stress on poultry production. Animals 2013;3:356-69. https://doi.org/10.3390/ani3020356
  2. He X, Lu Z, Ma B, et al. Effects of chronic heat exposure on growth performance, intestinal epithelial histology, appetiterelated hormones and genes expression in broilers. J Sci Food Agric 2018;98:4471-8. https://doi.org/10.1002/jsfa.8971
  3. Burkholder KM, Thompson KL, Einstein ME, Applegate TJ, Patterson JA. Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to Salmonella enteritidis colonization in broilers. Poult Sci 2008;87:1734-41. https://doi.org/10.3382/ps.2008-00107
  4. Nicholson JK, Holmes E, Kinross J, et al. Host-gut microbiota metabolic interactions. Science 2012;336:1262-7. https://doi.org/10.1126/science.1223813
  5. Clavijo V, Florez MJV. The gastrointestinal microbiome and its association with the control of pathogens in broiler chicken production: a review. Poult Sci 2018;97:1006-21. https://doi.org/10.3382/ps/pex359
  6. Koren O, Goodrich JK, Cullender TC, et al. Host remodeling of the gut microbiome and metabolic changes during pregnancy. Cell 2012;150:470-80. https://doi.org/10.1016/j.cell.2012.07.008
  7. Zhang ZY, Jia GQ, Zuo JJ, et al. Effects of constant and cyclic heat stress on muscle metabolism and meat quality of broiler breast fillet and thigh meat. Poult Sci 2012;91:2931-7. https://doi.org/10.3382/ps.2012-02255
  8. Aksit M, Yalcin S, Ozkan S, Metin K, Ozdemir D. Effects of temperature during rearing and crating on stress parameters and meat quality of broilers. Poult Sci 2006;85:1867-74. https://doi.org/10.1093/ps/85.11.1867
  9. Ito K, Erwan E, Nagasawa M, Furuse M, Chowdhury VS. Changes in free amino acid concentrations in the blood, brain and muscle of heat-exposed chicks. Br Poult Sci 2014;55:644-52. https://doi.org/10.1080/00071668.2014.957653
  10. Mardinoglu A, Shoaie S, Bergentall M, et al. The gut microbiota modulates host amino acid and glutathione metabolism in mice. Mol Syst Biol 2015;11:834. https://doi.org/10.15252/msb.20156487
  11. Committee on Nutrient Requirements of Poultry, National Research Council. Nutrient requirements of poultry. 9th revised ed. Washington, DC, USA: National Academies Press; 1994.
  12. Li X, Cao Z, Yang Y, et al. Correlation between jejunal microbial diversity and muscle fatty acids deposition in broilers reared at different ambient temperatures. Sci Rep 2019;9:11022. https://doi.org/10.1038/s41598-019-47323-0
  13. Alleman F, Leclercq B. Effect of dietary protein and environ-mental temperature on growth performance and water consumption of male broiler chickens. Br Poult Sci 1997;38:607-10. https://doi.org/10.1080/00071669708418044
  14. Kamel NN, Ahmed AMH, Mehaisen GMK, Mashaly MM, Abass AO. Depression of leukocyte protein synthesis, immune function and growth performance induced by high environmental temperature in broiler chickens. Int J Biometeorol 2017;61:1637-45. https://doi.org/10.1007/s00484-017-1342-0
  15. Samuels SE, Thompson JR, Christopherson RJ. Skeletal and cardiac muscle protein turnover during short-term cold exposure and rewarming in young rats. Am J Physiol Regul Integr Comp Physiol 1996;270:R1231-9. https://doi.org/10.1152/ajpregu.1996.270.6.R1231
  16. Scott SL, Christopherson RJ, Thompson JR, Baracos VE. The effect of a cold environment on protein and energy metabolism in calves. Br J Nutr 1993;69:127-39. https://doi.org/10.1079/BJN19930015
  17. Ma B, He X, Lu Z, et al. Chronic heat stress affects muscle hypertrophy, muscle protein synthesis and uptake of amino acid in broilers via insulin like growth factor-mammalian target of rapamycin signal pathway. Poult Sci 2018;97:4150-8. https://doi.org/10.3382/ps/pey291
  18. Smith OL, Huszar G, Davidson SB, Davis E. Effects of acute cold exposure on muscle amino acid and protein in rats. J Appl Physiol 1982;52:1250-6. https://doi.org/10.1152/jappl.1982.52.5.1250
  19. Lin H, Decuypere E, Buyse J. Acute heat stress induces oxidative stress in broiler chickens. Comp Biochem Physiol A Mol Integr Physiol 2006;144:11-7. https://doi.org/10.1016/j.cbpa.2006.01.032
  20. Luo S, Levine RL. Methionine in proteins defends against oxidative stress. FASEB J 2009;23:464-72. https://doi.org/10.1096/fj.08-118414
  21. Lan PTN, Sakamoto M, Benno Y. Effects of two probiotic Lactobacillus strains on jejunal and cecal microbiota of broiler chicken under acute heat stress condition as revealed by molecular analysis of 16S rRNA genes. Microbiol Immunol 2004;48:917-29. https://doi.org/10.1111/j.1348-0421.2004.tb03620.x
  22. Lozupone CA, Stombaugh JI, Gordon JI, Jansson JK, Knight R. Diversity, stability and resilience of the human gut microbiota. Nature 2012;489:220-30. https://doi.org/10.1038/nature11550
  23. Xu ZR, Hu CH, Xia MS, Zhan XA, Wang MQ. Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult Sci 2003;82:1030-6. https://doi.org/10.1093/ps/82.6.1030
  24. Wang XJ, Feng JH, Zhang MH, Li XM, Ma DD, Chang SS. Effects of high ambient temperature on the community structure and composition of ileal microbiome of broilers. Poult Sci 2018;97:2153-8. https://doi.org/10.3382/ps/pey032
  25. Torres C, Alonso CA, Ruiz-Ripa L, Leon-Sampedro R, Del Campo R, Coque TM. Antimicrobial resistance in Enterococcus spp. of animal origin. Microbiol Spectr 2018;6:185-227. https://doi.org/10.1128/microbiolspec.ARBA-0032-2018
  26. Arias CA, Murray BE. The rise of the Enterococcus: beyond vancomycin resistance. Nat Rev Microbiol 2012;10:266-78. https://doi.org/10.1038/nrmicro2761
  27. Le Roy T, Llopis M, Lepage P, et al. Intestinal microbiota determines development of non-alcoholic fatty liver disease in mice. Gut 2013;62:1787-94. https://doi.org/10.1136/gutjnl2012-303816
  28. Wylie KM, Truty RM, Sharpton TJ, et al. Novel bacterial taxa in the human microbiome. PLoS One 2012;7:e35294. https://doi.org/10.1371/journal.pone.0035294
  29. Neis EPJG, Dejong CH, Rensen SS. The role of microbial amino acid metabolism in host metabolism. Nutrients 2015;7:2930-46. https://doi.org/10.3390/nu7042930
  30. Lin R, Liu W, Piao M, Zhu H. A review of the relationship between the gut microbiota and amino acid metabolism. Amino Acids 2017;49:2083-90. https://doi.org/10.1007/s00726-017-2493-3