Journal of Microbiology and Biotechnology

  • 제31권11호
  • /
  • Pages.1501-1507
  • /
  • 2021
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPS-Induced ALI via Downregulating of MAPK and NF-κB Activation

  • Min, Jae-Hong (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Seong-Man (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, JI-Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Nam Hoon (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Goo, Soo Hyeon (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ngatinem, Ngatinem (Starch Technology Center, Agency for the Assessment and Application Technology) ;
  • Ningsih, Sri (Center for Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology) ;
  • Paik, Jin-Hyub (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Sangho (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Sei-Ryang (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Han, Sang-Bae (College of Pharmacy, Chungbuk National University) ;
  • Ahn, Kyung-Seop (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jae-Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 투고 : 2021.07.12
  • 심사 : 2021.09.01
  • 발행 : 2021.11.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Lagerstroemia ovalifolia Teijsm. & Binn. (LO) (crape myrtle) has reportedly been used as traditional herbal medicine (THM) in Java, Indonesia. Our previous study revealed that the LO leaf extract (LOLE) exerted anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Based on this finding, the current study aimed to evaluate the protective effects of LOLE in a mouse model of LPS-induced acute lung injury (ALI). The results showed that treatment with LPS enhanced the inflammatory cell influx into the lungs and increased the number of macrophages and the secretion of the inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) of mice. However, these effects were notably abrogated with LOLE pretreatment. Furthermore, the increase of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1) expression in the lung tissues of mice with ALI was also reversed by LOLE. In addition, LOLE significantly suppressed the LPS-induced activation of the MAPK/NF-κB signaling pathway and led to heme oxygenase-1 (HO-1) induction in the lungs. Additionally, in vitro experiments showed that LOLE enhanced the expression of HO-1 in RAW264.7 macrophages. The aforementioned findings collectively indicate that LOLE exerts an ameliorative effect on inflammatory response in the airway of ALI mice.

키워드

과제정보

This research was supported by grants from the Korea Research Institute of Bioscience and Biotechnology Research Initiative Program (KRIBB) (Grant No. KGM5522113 and KGS1402113) of the Republic of Korea.

참고문헌

  1. Butt Y, Kurdowska A, Allen TC. 2016. Acute lung injury: a clinical and molecular review. Arch. Pathol. Lab. Med. 140: 345-350. https://doi.org/10.5858/arpa.2015-0519-RA
  2. Huang X, Xiu H, Zhang S, Zhang G. 2018. The role of macrophages in the pathogenesis of ALI/ARDS. Mediators Inflamm. 13: 1264913
  3. Park JW, Ryu HW, Ahn HI, Min JH, Kim SM, Kim MG, et al. 2020. The anti-inflammatory effect of Trichilia martiana C. DC. in the lipopolysaccharide-stimulated inflammatory response in macrophages and airway epithelial cells and in LPS-challenged mice. J. Microbiol. Biotechnol. 30: 1614-1625. https://doi.org/10.4014/jmb.2006.06042
  4. Chen X, Tang J, Shuai W, Meng J, Feng J, Han Z. 2020. Macrophage polarization and its role in the pathogenesis of acute lung injury/acute respiratory distress syndrome. Inflamm. Res. 69: 883-895. https://doi.org/10.1007/s00011-020-01378-2
  5. Frank JA, Pittet JF, Lee H, Godzich M, Matthay MA. 2003. High tidal volume ventilation induces NOS2 and impairs cAMP-dependent air space fluid clearance. Am. J. Physiol. Lung. Cell. Mol. Physiol. 284: L791-798. https://doi.org/10.1152/ajplung.00331.2002
  6. Mehta S. 2005. The effects of nitric oxide in acute lung injury. Vascul. Pharmacol. 43: 390-403. https://doi.org/10.1016/j.vph.2005.08.013
  7. Giuliano F and Warner TD. 2002. Origins of prostaglandin E2: involvements of cyclooxygenase (COX)-1 and COX-2 in human and rat systems. J. Pharmacol. Exp. Ther. 303: 1001-1006. https://doi.org/10.1124/jpet.102.041244
  8. Laskin DL, Malaviya R, Laskin JD. 2019. Role of macrophages in acute lung injury and chronic fibrosis induced by pulmonary toxicants. Toxicol. Sci. 168: 287-301. https://doi.org/10.1093/toxsci/kfy309
  9. Xing J, Yu Z, Zhang X, Li W, Gao D, Wang J, et al. 2019. Epicatechin alleviates inflammation in lipopolysaccharide-induced acute lung injury in mice by inhibiting the p38 MAPK signaling pathway. Int. Immunopharmacol. 66: 146-153. https://doi.org/10.1016/j.intimp.2018.11.016
  10. Fang W, Cai SX, Wang CL, Sun XX, Li K, Yan XW, et al. 2017. Modulation of mitogen-activated protein kinase attenuates sepsis-induced acute lung injury in acute respiratory distress syndrome rats. Mol. Med. Rep. 16: 9652-9658. https://doi.org/10.3892/mmr.2017.7811
  11. Aggarwal NR, King LS, D'Alessio FR. 2014. Diverse macrophage populations mediate acute lung inflammation and resolution. Am. J. Physiol. Lung. Cell. Mol. Physiol. 306: L709-25. https://doi.org/10.1152/ajplung.00341.2013
  12. Lu Y, Xu D, Liu J, Gu L. 2019. Protective effect of sophocarpine on lipopolysaccharide-induced acute lung injury in mice. Int. Immunopharmacol. 70: 180-186. https://doi.org/10.1016/j.intimp.2019.02.020
  13. Lee JW, Chun W, Kwon OK, Park HA, Lim Y, Lee JH, et al. 2018. 3,4,5-Trihydroxycinnamic acid attenuates lipopolysaccharide (LPS)-induced acute lung injury via downregulating inflammatory molecules and upregulating HO-1/AMPK activation. Int. Immunopharmacol. 64: 123-130. https://doi.org/10.1016/j.intimp.2018.08.015
  14. Qing R, Huang Z, Tang Y, Xiang Q, Yang F. 2018 Cordycepin alleviates lipopolysaccharide-induced acute lung injury via Nrf2/HO-1 pathway. Int. Immunopharmacol. 60: 18-25. https://doi.org/10.1016/j.intimp.2018.04.032
  15. Hsieh YH, Deng JS, Pan HP, Liao JC, Huang SS, Huang GJ. 2017. Sclareol ameliorate lipopolysaccharide-induced acute lung injury through inhibition of MAPK and induction of HO-1 signaling. Int. Immunopharmacol. 44: 16-25. https://doi.org/10.1016/j.intimp.2016.12.026
  16. Lee JW, Seo KH, Ryu HW, Yuk HJ, Park HA, Lim Y, et al. 2018. Anti-inflammatory effect of stem bark of Paulownia tomentosa steud. in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS-induced murine model of acute lung injury. J. Ethnopharmacol. 210: 23-30. https://doi.org/10.1016/j.jep.2017.08.028
  17. Park HA, Kwon OK, Ryu HW, Min JH, Park MW, Park MH, et al. 2019. Physalis peruviana L. inhibits ovalbumin-induced airway inflammation by attenuating the activation of NF-κB and inflammatory molecules. Int. J. Mol. Med. 43: 1830-1838.
  18. Lee JW, Ryu HW, Park SY, Park HA, Kwon OK, Yuk HJ, et al. 2017. Protective effects of neem (Azadirachta indica A. Juss.) leaf extract against cigarette smoke- and lipopolysaccharide-induced pulmonary inflammation. Int. J. Mol. Med. 40: 1932-1940.
  19. Park JW, Kwon OK, Yuniato P, Marwoto B, Lee J, Oh SR, et al. 2016. Amelioration of an LPS-induced inflammatory response using a methanolic extract of Lagerstroemia ovalifolia to suppress the activation of NF-κB in RAW264.7 macrophages. Int. J. Mol. Med. 38: 482-490. https://doi.org/10.3892/ijmm.2016.2646
  20. Graham A, Graham SA, Nowicke JW, Patel V, Lee S. 1990. Palynology and systematics of the Lythraceae. III. genera physocalymma through woodfordia, addenda, and conclusions. Am. J. Bot. 77: 159-177. https://doi.org/10.2307/2444639
  21. Min JH, Kim MG, Kim SM, Park JW, Chun W, Lee HJ, et al. 2020. 3,4,5-Trihydroxycinnamic acid exerts a protective effect on pulmonary inflammation in an experimental animal model of COPD. Int. Immunopharmacol. 85: 106656. https://doi.org/10.1016/j.intimp.2020.106656
  22. Kim SM, Ryu HW, Kwon OK, Hwang D, Kim MG, Min JH, et al. 2021. Callicarpa japonica Thunb. ameliorates allergic airway inflammation by suppressing NF-κB activation and upregulating HO-1 expression. J. Ethnopharmacol. 267: 113523. https://doi.org/10.1016/j.jep.2020.113523
  23. Lin Y, Qiu D, Huang L, Zhang S, Song C, Wang B, et al. 2019. A novel chalcone derivative, L2H17, ameliorates lipopolysaccharideinduced acute lung injury via upregulating HO-1 activity. Int. Immunopharmacol. 71: 100-108. https://doi.org/10.1016/j.intimp.2019.02.002
  24. Lee JW, Chun W, Lee HJ, Min JH, Kim SM, Seo JY, et al. 2021. The role of macrophages in the development of acute and chronic inflammatory lung diseases. Cells 10: 897. https://doi.org/10.3390/cells10040897
  25. Lim Y, Park JW, Kwon OK, Lee JW, Lee HS, Lee S, et al. 2018. Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells. Int. J. Mol. Med. 41: 391-398.
  26. Li G, Dai Y, Tan J, Zou J, Nie X, Yang Z, et al. 2020. SB203580 protects against inflammatory response and lung injury in a mouse model of lipopolysaccharide-induced acute lung injury. Mol. Med. Rep. 22: 1656-1662. https://doi.org/10.3892/mmr.2020.11214
  27. Ju M, He H, Chen S, Liu Y, Liu Y, Pan S, et al. 2019. Ulinastatin ameliorates LPS-induced pulmonary inflammation and injury by blocking the MAPK/NF-κB signaling pathways in rats. Mol. Med. Rep. 20: 3347-3354.
  28. Zhong WT, Wu YC, Xie XX, Zhou X, Wei MM, Soromou LW, et al. 2013. Phillyrin attenuates LPS-induced pulmonary inflammation via suppression of MAPK and NF-κB activation in acute lung injury mice. Fitoterapia 90: 132-139. https://doi.org/10.1016/j.fitote.2013.06.003
  29. Liu Q, Ci X, Wen Z , Peng L. 2018. Diosmetin alleviates lipopolysaccharide-Induced acute lung injury through activating the Nrf2 pathway and inhibiting the NLRP3 inflammasome. Biomol. Ther (Seoul). 26: 157-166. https://doi.org/10.4062/biomolther.2016.234
  30. Ding H, Ci X, Cheng H, Yu Q, Li D. 2019. Chicoric acid alleviates lipopolysaccharide-induced acute lung injury in mice through anti-inflammatory and anti-oxidant activities. Int. Immunopharmacol. 66: 169-176. https://doi.org/10.1016/j.intimp.2018.10.042
  31. Wu KC, Huang SS, Kuo YH, Ho YL, Yang CS, Chang YS, et al. 2017. Ugonin M, a helminthostachys zeylanica constituent, prevents LPS-induced acute lung injury through TLR4-mediated MAPK and NF-κB signaling pathways. Molecules 22: 573. https://doi.org/10.3390/molecules22040573
  32. Lee JW, Park JW, Shin NR, Park SY, Kwon OK, Park HA, et al. 2016. Picrasma quassiodes (D. Don) Benn. attenuates lipopolysaccharide (LPS)-induced acute lung injury. Int. J. Mol. Med. 38: 834-844. https://doi.org/10.3892/ijmm.2016.2669
  33. Hong JM, Kwon OK, Shin IS, Jeon CM, Shin NR, Lee J, et al. 2015. Anti-inflammatory effects of methanol extract of Canarium lyi C.D. Dai & Yakovlev in RAW 264.7 macrophages and a murine model of lipopolysaccharide-induced lung injury. Int. J. Mol. Med. 35: 1403-1410. https://doi.org/10.3892/ijmm.2015.2117