구강회복응용과학지 (Journal of Dental Rehabilitation and Applied Science)

  • 제37권4호
  • /
  • Pages.244-250
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  • 2021
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  • 2384-4353(pISSN)
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  • 2384-4272(eISSN)
대한턱관절교합학회 (Korean Academy of Stomatognathic Function and Occlusion)
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Streptococcus salivarius K12의 배양조건에 따른 항균활성의 특징

Characteristics of antimicrobial activity of Streptococcus salivarius K12 by culture condition

  • 송영균 (단국대학교 치과대학 보철학교실) ;
  • 이성훈 (단국대학교 치과대학 구강미생물학교실)
  • Song, Young-Gyun (Department of Prosthodontics, College of Dentistry, Dankook University) ;
  • Lee, Sung-Hoon (Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University)
  • 투고 : 2021.12.09
  • 심사 : 2021.12.10
  • 발행 : 2021.12.31
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초록

목적: 본 연구의 목적은 Streptococcus salivarius K12의 성장과 항균활성에 대한 배양조건의 영향을 알아보는 것이다. 연구 재료 및 방법: S. salivarius K12는 동물 또는 식물 단백질을 함유한 배지 또는 중성 및 산성 조건의 배지에서 배양되었다. S. salivarius K12의 성장은 2시간마다 분광광도계로 측정하였다. S. salivarius K12의 Streptococcus mutans, Porphyromonas gingivalis 및 Candida albicans에 대한 항균 또는 항진균 활성을 배양액을 이용한 감수성 분석으로 조사하였다. 결과: S. salivarius K12의 성장은 식물 단백질과 중성 pH 조건에서 더 빠른 성장을 보였다. S. salivarius K12의 항균 및 항진균 활성은 동물성 단백질보다 식물성 단백질을 함유한 배지에서 더 강하게 나타났다. 결론: S. salivarius K12를 세균성 구강질환에 적용하기 위해서는 S. salivarius K12가 구강 내에서 군집화하여 항균 활성을 향상시키기 위한 보조물질이 필요할 수 있다.

Purpose: The purpose of this study was to examine effects of culture conditions on the growth and antibacterial activity of Streptococcus salivarius K12. Materials and Methods: S. salivarius K12 was cultivated in medium containing animal and plant protein or in medium of neutral and acidic conditions. The growth of S. salivarius K12 was measured every 2 hours by a spectrophotometer. The antimicrobial activity of S. salivarius K12 against Streptococcus mutans and Porphyromonas gingivalis was investigated by the susceptibility assay using the spent culture medium. Results: the growth of S. salivarius K12 showed faster in medium containing plant protein and neutral pH condition. The antimicrobial and antifungal activity of S. salivarius K12 appeared stronger in medium containing plant protein than animal proteins. Conclusion: For application of S. salivarius K12 to bacterial oral disease, co-substances may be needed for S. salivarius K12 to colonize in the oral cavity and enhance the antimicrobial activity.

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