Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Virulence factors and multi-drug resistant patterns of pathogenic Escherichia coli isolates from diarrheic calves in Jeonbuk

전북지역 송아지 설사 유래 병원성 대장균의 병원성 인자 및 다제 내성 패턴

  • Kwak, Kil-Han (Animal Health Institute of Jellabukdo) ;
  • Kim, Seon-Min (Department of Veterinary Public Health, College of Veterinary Medicinee, Jeonbuk National University) ;
  • Yu, Yeong-Ju (Department of Veterinary Public Health, College of Veterinary Medicinee, Jeonbuk National University) ;
  • Yu, Jeong-Hee (Department of Veterinary Public Health, College of Veterinary Medicinee, Jeonbuk National University) ;
  • Lim, Mi-Na (Animal Health Institute of Jellabukdo) ;
  • Jang, Yu-Jeong (Animal Health Institute of Jellabukdo) ;
  • Hur, Jin (Department of Veterinary Public Health, College of Veterinary Medicinee, Jeonbuk National University)
  • 곽길한 (전라북도 동물위생시험소 서부지소) ;
  • 김선민 (전북대학교 수의과대학 수의공중보건학실) ;
  • 유영주 (전북대학교 수의과대학 수의공중보건학실) ;
  • 유정희 (전북대학교 수의과대학 수의공중보건학실) ;
  • 임미나 (전라북도 동물위생시험소 서부지소) ;
  • 장유정 (전라북도 동물위생시험소 서부지소) ;
  • 허진 (전북대학교 수의과대학 수의공중보건학실)
  • Received : 2021.12.17
  • Accepted : 2021.12.24
  • Published : 2021.12.30
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Abstract

Pathogenic Escherichia coli (E. coli) is one among the most important agents of diarrhea in calves. From January to December 2021, 108 isolates from feces of calves with diarrhea were investigated for enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC), and enteroinvasive E. coli (EIEC) using real-time PCR. In addition, the genes for F5, F17 and F41 fimbriae were detected by PCR. The most frequently isolated pathotypes were EPEC/STEC (29 isolates), and ETEC/EPEC/STEC (29 isolates). ETEC/EPEC, and ETEC/STEC were also found in 10 isolates. EPEC, STEC, and ETEC were detected in 13, 11, and 6 respectively. EAEC, and EIEC was not detected. Antimicrobial resistance test was carried out by agar disc diffusion method with 14 antimicrobials. Among 108 pathogenic E. coli isolates, 107 isolates were resistant to at least one of 14 antibiotics used in this study, 99 (91.7%) were resistant to two or more antimicrobials, and a single remarkable isolate was resistant to 14 antimicrobials. The isolates were primarily resistant to penicillins, streptomycin, tetracycline, ceftiofur, Trimethoprim/sulfamethoxazole, Kanamycin, and Ciprofloxacin. The high rate of resistance in pathogenic E. coli, sometimes to multiple drugs, may complicate future options for treating human infections. These results may bu used for diagnosis and therpeitic purposes in calves with diarrhea.

Keywords

Acknowledgement

이 논문은 정부의 재원으로 농림식품기술기획평가원(Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry, IPET)(No. 320070-2)과 전라북도 동물위생시험소 서부지소의 지원을 받아 연구되었습니다.

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