The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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The Physiological Activity of Crude Polysaccharide Solvent Extracted from Herbal Medicine Mixture

생약복합물 용매추출물과 조다당획분의 생리활성

  • Shin, Hyun-Young (Dept. of Integrated Biomedical and Life Science, Korea University) ;
  • Kim, Hoon (Skin Biotechnology Center, Kyung Hee University) ;
  • Shin, Ji-Young (Cosis-Bio Co. Ltd.) ;
  • Lee, Sue Jung (Major in Food Science and Biotechnology, Kyonggi University) ;
  • Yu, Kwang-Won (Major in Food and Nutrition, Korea National University of Transportation)
  • 신현영 (고려대학교 의생명융합과학과) ;
  • 김훈 (경희대학교 피부생명공학센터) ;
  • 신지영 ((주)코시스바이오 연구소) ;
  • 이수정 (경기대학교 식품생물공학전공) ;
  • 유광원 (한국교통대학교 식품영양학전공)
  • Received : 2020.12.02
  • Accepted : 2021.01.11
  • Published : 2021.02.28
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Abstract

After ethanol (BM-E and RW-E) and hot-water (BM-HW and RW-HW) extracts were fractionated from two herbal mixtures (BM and RW), their physiological activities were investigated. All extracts consisted of more than 50% of neutral sugar, with their total polyphenol levels higher than flavonoid levels. Radical scavenging activities of EtOH extracts remained significantly higher compared to that of hot-water extracts, and in particular, RW-E showed consistently higher antioxidant activity than BM-E. When anti-inflammatory activities of the extracts were evaluated by LPS-stimulated RAW 264.7 cells at 10~500 μg/mL non-cytotoxicity doses, BM-E showed significantly higher levels of TNF-α, IL-1β, IL-6, and nitric oxide inhibitory activity than those of hot-water extracts and RW-E. Murine peritoneal macrophage cells were shown to be enhanced in crude polysaccharides (BM-CP and RW-CP fractionated from BM-HW and RW-HW) compared to hot-water extracts and polysaccharide K (PSK, positive control). Especially, RW-CP exhibited higher activity than BM-CP, and component sugar analysis showed that BM-CP mainly contained galacturonic acid, glucose, arabinose, galactose, and xylose (34.5%, 33.9%, 16.1%, 7.1%, and 6.3%, respectively), whereas RW-CP showed different measurements (29.5%, 59.2%, 5.0%, 4.5%, and 0.2%). In conclusion, two herbal mixtures could contain varying sets of physiological activities dependent on different extraction and fractionation methods.

Keywords

Acknowledgement

2020년 한국교통대학교와 (주)코시스바이오의 지원을 받아 수행하였습니다.

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