한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
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불레기말(Colpomenia sinuosa)의 최종당화산물 저해 효능 및 신장 보호 효과

Anti-glycation effect and renal protective activity of Colpomenia sinuosa extracts against advanced glycation end-products (AGEs)

  • 김민경 (과학기술연합대학원대학교 식품생명공학전공) ;
  • 조치흥 (한국식품연구원 식품기능연구본부) ;
  • 김세라 (한국식품연구원 식품기능연구본부) ;
  • 최인욱 (한국식품연구원 식품기능연구본부) ;
  • 이상훈 (한국식품연구원 식품기능연구본부)
  • Kim, Mingyeong (Department of Food Biotechnology, University of Science and Technology) ;
  • Cho, Chi Heung (Division of Food Functionality Research, Korea Food Research Institute) ;
  • Kim, Sera (Division of Food Functionality Research, Korea Food Research Institute) ;
  • Choi, In-Wook (Division of Food Functionality Research, Korea Food Research Institute) ;
  • Lee, Sang-Hoon (Division of Food Functionality Research, Korea Food Research Institute)
  • 투고 : 2021.11.22
  • 심사 : 2021.12.24
  • 발행 : 2021.12.31
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초록

Here, we evaluated the anti-glycation effects and renal protective properties of 70% (v/v) ethanolic extract of Colpomenia sinuosa (CSE) against AGEs -induced oxidative stress and apoptosis at different concentrations (1, 5, and 20 ㎍/mL). At 20 ㎍/mL, CSE showed that anti-glycation activities via the inhibition of AGE formation (51.1%), inhibition of AGEs-protein cross-linking (61.7%), and breaking of AGEs-protein cross-links (33.3%), were significantly (###p < 0.001 vs. non-treated group) lower than the nontreated group. Methylglyoxal (MGO) significantly (***p < 0.001) reduced cell viability (24.4%) and increased reactive oxygen species (ROS) level (642.3%), MGO accumulation (119.4 ㎍/mL), and apoptosis (55.0%) in mesangial cells compared to the nontreated group. Pretreatment with CSE significantly (###p < 0.001) increased cell viability (57.8%) and decreased intracellular ROS (96.5%), MGO accumulation (80.0 ㎍/mL), and apoptosis (22.6%) at 20 ㎍/mL. Additionally, we confirmed intracellular AGEs reduction by CSE pretreatment. Consequently, our results suggest that CSE is a good source of natural therapeutics for managing diabetic complications by the antiglycation effect and renal protective activity against MGO-induced oxidative stress.

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과제정보

본 연구성과는 정부(과학기술정보통신부)의 재원으로 한국연구재단(NRF-2020R1A2C201260811)의 지원을 받아 수행된 연구임

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