Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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The effect of wound healing of Nile tilapia (Oreochromis niloticus) using PDRN (polydeoxyribonucleotide) extracted from seaweed (Porphyra sp.)

김(Porphyra sp.)에서 추출한 PDRN (polydeoxyribonucleotide)의 나일틸라피아(Oreochromis niloticus)에서의 상처 치유 효과

  • Yang, Chan Yeong (Departments of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Han, Ji Sung (All-in-one Jintec) ;
  • Lee, Won Se (All-in-one Jintec) ;
  • Bae, Jun Sung (Departments of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Lee, Chae Won (Departments of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Jeong, Eun Ha (Departments of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Kim, Gwan Hui (Departments of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Park, Kwan Ha (Departments of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 양찬영 (군산대학교 해양과학대학 수산생명의학과) ;
  • 한지성 (올인원진텍) ;
  • 이원세 (올인원진텍) ;
  • 배준성 (군산대학교 해양과학대학 수산생명의학과) ;
  • 이채원 (군산대학교 해양과학대학 수산생명의학과) ;
  • 정은하 (군산대학교 해양과학대학 수산생명의학과) ;
  • 김관희 (군산대학교 해양과학대학 수산생명의학과) ;
  • 박관하 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2021.08.27
  • Accepted : 2021.09.30
  • Published : 2021.12.31
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Abstract

Polydeoxyribonucleotide (PDRN) is known to accelerate wound healing process by acting on specific adenosine receptors. Differently from the PDRN substances obtained so far from piscine tissues, unique PDRN was prepared recently from Porphyra, a seaweed species. In this study, effects of the Porphyra PDRN was evaluated by inducing artificial skin wounds in Nile tilapia Oreochromis niloticus. The wound recovered almost completely in 2 weeks without any treatment, however only a partial recovery was done after one week. Facilitated healing was observed by PDRN fed for 5 days before wound induction at 10 or 20 mg/kg, against the partially recovered 1-week old wound. The healing effects were identified not only by visual observation, wound area measurement, but also by colorimetric estimations. These novel wound healing effects suggest beneficial therapeutic utility of Porphyra-derived PDRN for wound treatments in fishes occurring from various causes.

Polydeoxyribonucleotide (PDRN)은 특정 adenosine 수용체에 작용하여 상처 치유 과정을 촉진하는 것으로 알려져 있다. 지금까지 어류의 조직에서 얻어진 PDRN과는 다르게, 최근 해초류인 Porphyra로부터 추출된 PDRN이 개발되었다. 본 연구는 나일틸라피아(Oreochromis niloticus)에서 인공적으로 피부의 상처를 유발한 뒤 Porphyra에서 추출한 PDRN을 이용하여 상처 치유 효과를 평가하였다. 상처의 회복은 시험 시작 후 1주내에는 일부만 회복되었지만, 2주내에 거의 완전히 회복되었다. 상처를 유발하기 전 PDRN을 10 및 20 mg/kg의 용량으로 5일간 사료로 투여한 후 상처 치유 효과를 관찰하였다. 상처 치유 효과는 육안적, 면적 및 색도측정으로 평가하였다. 본 연구에서 관찰된 Porphyra 유래의 PDRN에 의한 상처 치유 효과는 다양한 원인에 의해 발생하는 어류의 상처 치유 목적으로의 활용 가능성을 보여준다.

Keywords

Acknowledgement

본 논문은 2021년도 군산대학교 자체 국내연구교수 학술경비로 지원되었음.

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