Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Quantitative analysis of myxosporean parasites (Enteromyxum leei and Parvicapsula anisocaudata) detected from emaciated olive flounder (Paralichthys olivaceus) and rearing water

여윔증상 넙치 및 사육수 내 검출된 점액포자충(Enteromyxum leei and Parvicapsula anisocaudata)의 정량적 분석

  • Lee, Young Juhn (Dept. of Marine Life Sciences, Jeju National University) ;
  • Jun, Lyu Jin (Dept. of Marine Life Sciences, Jeju National University) ;
  • Kim, Ye Ji (Dept. of Marine Life Sciences, Jeju National University) ;
  • Han, Ji Eun (Dept. of Marine Life Sciences, Jeju National University) ;
  • Lee, Eung Jun (Dept. of Marine Life Sciences, Jeju National University) ;
  • Jeong, Joon Bum (Dept. of Marine Life Sciences, Jeju National University)
  • 이영준 (제주대학교 해양생명과학과) ;
  • 전려진 (제주대학교 해양생명과학과) ;
  • 김예지 (제주대학교 해양생명과학과) ;
  • 한지은 (제주대학교 해양생명과학과) ;
  • 이응준 (제주대학교 해양생명과학과) ;
  • 정준범 (제주대학교 해양생명과학과)
  • Received : 2021.11.21
  • Accepted : 2021.11.29
  • Published : 2021.12.31
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Abstract

Quantitative analysis of myxosporean parasites (Enteromyxum leei and Parvicapsula anisocaudata) were performed using real-time PCR on the internal organs (head kidney, body kidney, intestine, spleen, brain, liver, heart, muscle, blood, and eye) of emaciated Paralichthys olivaceus from farm-A. The highest DNA copy number of E. leei was shown in the intestine (1.3 × 108 copies/mg tissue) of emaciatied P. olivaceus and DNA copy number in the other internal organs (1.3 × 103~4.6 × 105 copies/mg tissue) showed lower than in intestine. From the result of real-time PCR for P. anisocaudata, it was considered mildly infected, due to the low DNA copy numbers of the head kidney (1.3 × 103 copies/mg tissue) and body kidney (9.1 × 103 copies/mg tissue). In order to investigate whether myxosporean parasites can be detected in a non-invasive way, quantitative analysis of E. leei and P. anisocaudata from rearing water of three farms were performed by real-time PCR. The DNA copy number of E. leei from rearing water of farm-A and farm-B were 8 × 104 and 5 × 105 copies/L, respectively. However, it was not detected in farm-C. For P. anisocaudata from rearing water, farm-A, farm-B and farm-C showed 0, 2.0 × 106 and 5.1 × 106 copies/L, respectively.

Keywords

Acknowledgement

이 논문은 2021년 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구임(넙치 SPF(특정 병원체 무감염) 종자생산 기술개발).

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