대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)

  • 제53권4호
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  • Pages.353-362
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  • 2021
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  • 1738-3544(pISSN)
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  • 2288-1662(eISSN)
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)
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MNNG 처리에 의해 조절되는 암발생 유발 유전자의 조사

MNNG-Regulated Differentially Expressed Genes that Contribute to Cancer Development in Stomach Cells

  • 김태진 (호서대학교 임상병리학과) ;
  • 김명관 (호서대학교 임상병리학과) ;
  • 정동주 (호서대학교 임상병리학과)
  • Kim, Tae-Jin (Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University) ;
  • Kim, Myeong-Kwan (Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University) ;
  • Jung, Dongju (Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University)
  • 투고 : 2021.10.22
  • 심사 : 2021.11.21
  • 발행 : 2021.12.31
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초록

암은 전 세계적인 건강문제이다. 암의 종류는 다양하나 암의 발생과정에서의 유사성은 상당히 높다. 모든 암은 유전자의 발현 변화가 있다는 점에서 공통점을 갖는다. 암 발생과정에서 변화되는 유전자의 발현을 이해하는 것은 암치료제나 암백신 개발에 큰 도움을 줄 것이다. 이번 연구에서는 잘 알려진 암발생 물질인 MNNG를 이용하여 정상 위세포에서 암발생 시 변화되는 유전자 발현을 분석하였다. MNNG 처리에 의해 정상 세포와는 다르게 발현되는 유전자인 DEG들을 찾고 이들을 암세포에서 발현되는 DEG들과 비교하였다. 여기에 더해 DEG의 단백질 결과물들의 기능과 단백질들 간의 결합을 분석하여 단순히 유전자의 발현뿐만 아니라 이들 단백질의 신호전달 과정에서의 연관성도 함께 분석하였다. 이 결과 위암 발생과정에서 관여하는 유전자들과 이들 유전자의 단백질 결과물들의 상호 작용을 밝히게 되었다.

Cancer is a global health problem. There are diverse types of cancers, but there are several common pathways which lead to the development of cancer. Changes in gene expression might be the most common similarity found in almost all cancers. An understanding of the underlying changes in gene expression during cancer progression could lay a valuable foundation for the development of cancer therapeutics and even cancer vaccines. In this study, a well-known carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), was employed to induce changes in gene expression in normal stomach cells. MNNG is known to cause cancer by inducing damage to DNA in MNNG-treated mammalian cells and animals fed with this carcinogen. An analysis was performed by comparing the differentially expressed genes (DEGs) caused by MNNG treatment with DEGs in stomach cancer cell lines. To this end, methods of analysis for functional categorization and protein-protein interaction networks, such as gene ontology (GO), the database for annotation, visualization, and integrated discovery (DAVID), Kyoto encyclopedia of genes and genomics (KEGG) and search tool for the retrieval of interacting genes/proteins (STRING), were used. As a result of these analyses, MNNG-regulated specific genes and interaction networks of their protein products that contributed to stomach cancer were identified.

키워드

과제정보

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) grant (http://nrf.re.kr) to Dongju Jung (NRF-2019R1I1A3A01061981).

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