Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Bisphenol a induces reproductive dysfunction in male mice

  • Young-Joo, Yi (Department of Agricultural Education, College of Education, Sunchon National University) ;
  • Malavige Romesha, Chandanee (Department of Agricultural Education, College of Education, Sunchon National University) ;
  • Dong-Won, Seo (Department of Vaccine, Gyeongbuk Institute for Bio industry) ;
  • Jung-Min, Heo (College of Agriculture and Life Sciences, Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Min, Cho (Division of Biotechnology, SELS center, College of Environmental & Bioresource Sciences, Jeonbuk National University) ;
  • Sang-Myeong, Lee (Laboratory of Veterinary Virology, College of Veterinary Medicine, Chungbuk National University)
  • Received : 2021.10.21
  • Accepted : 2021.11.11
  • Published : 2021.12.01
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Abstract

It has been suggested that bisphenol A (BPA), a known endocrine disruptor, interferes with the endocrine system, causing reproductive dysfunction. Recently, BPA has been found in waste water due to incomplete sewage purification, possibly threatening health through its ingestion via tap water. In this study, young male mice (6 - 7 weeks old) were administered water containing BPA (50 mg·kg-1) for four weeks, while control mice consumed water without BPA. Serum, epididymal spermatozoa and testicular sections were assessed after sacrificing the mice on day 28. No significant differences were obtained between the groups in the body, testis and seminal vesicle weights. However, the epididymal sperm motility and count levels were significantly reduced in BPA-fed mice. Significantly higher hepatotoxicity levels were also observed in mice ingesting BPA as compared to the control mice. The level of serum testosterone was reduced, and testicular sections revealed incomplete and irregular spermatogenesis in BPA-ingested mice. The sperm proteasomal-proteolytic activity level has been implicated in sperm function and is measured in motile spermatozoa using fluorometric substrates. High ubiquitin C-terminal hydrolase activity levels were observed in the control mice without BPA. During a mating trial, a low pregnancy rate (71.4%) was observed in females mated with males who had consumed BPA (100% in the control mice). Overall, BPA adversely affected spermatogenesis and quality, as indicated by decreased sperm motility, concentration and serum testosterone levels, resulting in reduced fertility competence.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government(MSIT) (NRF-2013R1A6A3A04063769 and NRF-2020R1A2C1014007).

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