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Quality characteristics and physiological activities of strawberry vinegars using Acetobacter pasteurianus SRCM60009

Acetobacter pasteurianus SRCM60009로 발효한 딸기 식초의 품질특성 및 생리활성

  • 임은정 ((재)발효미생물산업진흥원) ;
  • 조승화 ((재)발효미생물산업진흥원) ;
  • 강현진 ((재)발효미생물산업진흥원) ;
  • 박슬기 ((재)발효미생물산업진흥원) ;
  • 정도연 ((재)발효미생물산업진흥원)
  • Received : 2021.09.17
  • Accepted : 2021.11.17
  • Published : 2021.12.31

Abstract

Strawberries fermented with Acetobacter pasteurianus SRCM60009 were prepared, and the quality characteristics and physiological activity were measured. As the fermentation period increased, viable cell counts increased, pH decreased, and total acidity increased from 1.09% to 4.20%. The organic acid content of strawberry through acetic acid fermentation was confirmed in the following order: acetic acid, succinic acid, citric acid, and lactic acid. Measurement of α-glucosidase and pancreatic lipase inhibitory activities and angiotensin converting enzyme inhibitory activity showed significantly increased physiological activity owing to fermentation. The use of strawberry vinegar as a functional material was confirmed by measuring the anti-diabetic, anti-obesity, and anti-hypertensive physiological activities through acetic acid fermentation of strawberry. Thus, fermented strawberry vinegar can be used as a functional material in vinegar and other foods.

고부가가치 딸기 식초를 개발하고 이를 다양한 식품에 활용하기 위해 딸기 발효에 적합한 초산균을 선발하여 딸기 식초를 제조하고 품질 특성 및 생리활성을 분석하였다. A. pasteurianus 균주를 이용하여 딸기 식초를 제조하여 균주별 발효 특성을 조사한 결과 SRCM 60009 균주로 발효 후 산도가 가장 높은 결과를 보여 A. pasteurianus SRCM 60009 균주가 딸기 식초용 발효균주로 최종 선발되었다. SRCM 60009 균주로 제조한 딸기 발효식초의 품질특성은 발효 9일차에 pH 2.86, 산도 4.20%, 생균수 8.56 log CFU/mL를 나타내었다. 발효기간에 따라 딸기 식초의 유리당은 감소하는 경향을 보였고, 유기산은 증가하는 경향을 나타내었다. 딸기 식초의 생리활성은 α-glucosidase 저해(AGI) 활성, pancreatic lipase 저해(PLI) 활성, angiotensin converting enzyme I (ACE) 저해활성을 분석하였다. 딸기 식초는 65.13%의 AGI 활성을 보였으나, 딸기 착즙액을 발효하지 않은 대조구에서는 55.18%의 활성을 나타내었다. PLI 활성을 확인한 결과 딸기 식초는 75.40%, 대조구는 35.79%의 활성을 보였고, ACE 저해 활성은 딸기식초는 70.58%, 대조구는 46.40%의 활성을 보였으며, 그 결과는 모두 유의차가 있는 것으로 확인되었다. 이와 같이 딸기를 발효하여 식초로 이용함으로써 딸기를 발효하지 않고 섭취하는 것에 보다 다양한 기능성을 확인할 수 있었으며 동시에 전통 발효식품인 식초에 딸기의 맛과 향이 더해진 딸기 식초를 제조하였다. 이를 토대로 향후 다양한 소비자들의 기호를 충족시킬 수 있는 발효식초 및 발효식초를 활용한 응용제품의 개발이 필요하며, 이에 따른 추가적인 연구가 지속되어야 할 것 으로 사료된다.

Keywords

Acknowledgement

본 연구는 농림축산식품부의 지역전략식품산업육성사업(2020-01)의 지원에 의해 수행되었습니다.

References

  1. Ann YG, Oh MH, Lee BY. Vinegar produced from Chrysanthemum zawadskii and pearl shell. Korean J. Food Nutr. 25: 90-98 (2012) https://doi.org/10.9799/KSFAN.2012.25.1.090
  2. Bae SM, Han SM, Choi JM, Lee JS, Kim HK. Manufacturing of Korean traditional rice wine, Makgeolli, Supplemented with strawberry and its physicochemical and microbial properties during fermentation. Kor. J. Mycol. 44: 307-313 (2016) https://doi.org/10.4489/KJM.2016.44.4.307
  3. Bae IY, Shin HK, Yang CB. Studies on the inhibitory activity of angiotensin I converting enzyme of various vegetables. J. Life Sci. 17: 5-15 (1999)
  4. Baek CH, Jeong DH, Baek SY, Choi JH, Park HY, Choi HS, Jeong ST, Kim JH, Jeong YJ, Kwon JH, Yeo SH. Quality characteristics of farm-made brown rice vinegar via traditional static fermentation. Korean J. Food Preserv. 20: 564-572 (2013) https://doi.org/10.11002/KJFP.2013.20.4.564
  5. Baron AD. Postprandial hyperglycemia and α-glucosidase inhibitors. Diabetes Res. Clin. Pr. 40: 51-55 (1998) https://doi.org/10.1016/S0168-8227(98)00043-6
  6. Caner C, Aday MS, Demir M. Extending the quality of fresh strawberries by equilibrium modified atmosphere packaging. Eur. Food Res. Technol. 227: 1575-1583 (2008) https://doi.org/10.1007/s00217-008-0881-3
  7. Cha YJ, Park KJ, Kim DK, Chun HS, Lee BK, Kim KH, Lee SY. Kim SJ. Isolation and characterization of cellulose producing Acetobacter xylinum KI strain. Kor. J. Appl. Microbiol. Biotechnol. 22: 571-576 (1994)
  8. Cho JH. Letter: Balsamic vinegar improves high fat-induced beta cell dysfunction via beta cell ABCA-1. Diabetes Metab. 36: 388-389 (2012) https://doi.org/10.4093/dmj.2012.36.5.388
  9. Cho EK, Song HJ, Cho HE, Kim MH, Choi IS, Choi YJ. Inhibitory effects of ethanol extracts from pine buds (Pinus densiflora) on angiotensin converting enzyme, xanthine oxidase and nitric oxide synthesis. J. Life Sci. 19: 1629-1636 (2009) https://doi.org/10.5352/JLS.2009.19.11.1629
  10. Drent ML, Larsson I, Olsson TW, Quaade F, Czubayko K, Bergman KV, Strobel W, Sjostrom L, Veen EAVD. Orlistat (RO 18-0647), a lipase inhibitor, in the treatment of human obesity: A multiple dose study. Int. J. Obes. Relat. Metab. Disord. 19: 221-226 (1995)
  11. Holcroft, DM, Kader AA. Controlled atmosphere-induced changes in pH and organic acid metabolism may affect color of stored strawberry fruit. Postharvest Biol. Technol. 17:19-32 (1999) https://doi.org/10.1016/S0925-5214(99)00023-X
  12. Hong SM, Kang MJ, Lee JH, Jeong JH, Kwon SH, Seo KI. Production of vinegar using Rubus coreanus and its antioxidant activities. Korean J. Food Preserv. 19: 594-603 (2012a) https://doi.org/10.11002/KJFP.2012.19.4.594
  13. Hong SM, Moon HS, Lee JH, Lee HI, Jeong JH, Lee MK, Seo KI. Development of functional vinegar by using cucumbers. J. Korean Soc. Food Sci. Nutr. 41: 927-935 (2012b) https://doi.org/10.3746/JKFN.2012.41.7.927
  14. Jang JH, Na KC, Kim WS, Lee JS. Manufacture and characteristics of functional drink using pear-strawberry fermentative concentrates from fermentation by Saccharomyces cerevisiae C-2. Kor. J. Mycol. 38: 189-191 (2010) https://doi.org/10.4489/KJM.2010.38.2.189
  15. Jeong YJ, Lee MH, Seo KI, Kim JN, Lee YS. The quality comparison of grape vinegar by two stages fermentation with traditional grape vinegar. J. East Asian Soc. Dietary Life 8: 462-468 (1998)
  16. Jeong YJ, Lee MH. A view and prospect of vinegar industry. Food Ind. Nutr. 5: 7-12 (2000)
  17. Jeong YJ, Seo JH, Park NY, Shin SR, Kim KS. Changes in the components of persimmon vinegars by two stages fermentation (I). Korean J. Postharvest Sci. Technol. 6: 228-232 (1999)
  18. Jeong YJ. Current trends and future prospects in the Korean vinegar industry. Food Sci. Ind. 42: 52-59 (2009) https://doi.org/10.23093/FSI.2009.42.2.52
  19. Jung NJ, Kang YH. Comparison of the physicochemical quality characteristics of strawberry jams by processing methods. Korean J. Food Preserv. 19: 337-343 (2012) https://doi.org/10.11002/KJFP.2012.19.3.337
  20. Jung SA, Kim KBWR, Kim DH, Cho JY, Kim TW, Ahn DH. Lipase inhibitory mode of dieckol isolated from Eisenia bucyclis ethanol extract. Korean J. Microbiol. Biotechnol. 41: 112-118 (2012)
  21. Kim MJ, Choi JH, Kwon SH, Kim HD, Bang MH, Yang SA. Characteristics of fermented dropwort extract and vinegar using fermented dropwort extract and its protective effects on oxidative damage in rat glioma C6 cells. Korean J. Food Sci. Technol. 45: 350-355 (2013) https://doi.org/10.9721/KJFST.2013.45.3.350
  22. Kim JI, Han DW, Yun JA, Beak HJ, Lim SW. Pancreatic lipase inhibitory activity and antioxidant activity of carrot vinegar. Korean Soc. Biotech. Bioeng. J. 33: 104-109 (2018)
  23. Kim YH, Joo JI, Lee BC, Kim HH, Lee JS. Screen of a novel yeast for brewing of Gugija leaf Makgeolli and optimal alcohol fermentation condition. Kor. J. Mycol. 41:167-71 (2013) https://doi.org/10.4489/KJM.2013.41.3.167
  24. Kim JS, Kang EJ, Chang YE, Lee JH, Kim GG, Kim KM, Characteristics of strawberry jam contating strawberry puree. Korean J. Food Cook. Sci. 29: 725-731 (2013) https://doi.org/10.9724/kfcs.2013.29.6.725
  25. Kim HJ, Park SH, Park CH. Studies on the production of vinegar from barley. Korean J. Food Sci. Technol. 17: 350-354 (1985)
  26. Kim JH, Quality characteristics and physiological activities of black rice vinegar. Master thesis, Sunchon National University, Suncheon-si, Jeollanam-do, Republic of Korea (2017)
  27. Kwon SH, Jeong EJ, Lee GD, Jeong YJ. Preparation method of fruit vinegars by two stage fermentation and beverages including vinegar. Food Ind. Nutr. 5:18-24 (2000)
  28. Lee JH, Chang KS, Yoon HK. Studies on color and rheological properties in strawberry jam. Res. Rep. Agri. Sci. Tech. 14: 134-143 (1987)
  29. Lee MK, Choi SR, Lee J, Choi YH, Lee JH, Park KU, Kwon SH, Seo KI. Quality Characteristics and Anti-Diabetic Effect of Yacon Vinegar. J. Korean Soc. Food Sci. Nutr. 41: 79-86 (2012) https://doi.org/10.3746/JKFN.2012.41.1.079
  30. Lee YC, Jang OY, Kim HW, Choi CU, Yoon SK. Physicochemical characteristics of traditional vinegars in Andong province. Korean J. Dietary culture. 14: 17-20 (1999)
  31. Lee SH, Kim JC. A comparative analysis for main components change during natural fermentation of persimmon vinegar. J. Korean Soc. Food Sci. Nutr. 38: 372-376 (2009) https://doi.org/10.3746/JKFN.2009.38.3.372
  32. Lee JM, Kim SK, Lee GD. Monitoring on alcohol fermentation characteristics of strawberry. J. Korean Soc. Food Sci. Nutr. 32: 679-683 (2003a) https://doi.org/10.3746/JKFN.2003.32.5.679
  33. Lee KD, Kim SK, Lee JM. Optimization of the acetic acid fermentation condition for preparation of strawberry vinegar. J. Korean soc. Food Sci. Nutr. 32: 812-817 (2003b) https://doi.org/10.3746/JKFN.2003.32.6.812
  34. Lee MY, Kim HY, Singh D, Yeo SH, Paek SY, Park YK, Lee CH. Metabolite Profiling Reveals the Effect of Dietary Rubus coreanus Vinegar on Ovariectomy-Induced Osteoporosis in a Rat Model. Molecules 21: 149 (2016) https://doi.org/10.3390/molecules21020149
  35. Lee DS, Ryu IH, Lee GS, Shin YS, Joen SH. Optimization effect against lipase activity in preparation of aloe vinegar by Acetobacter sp. and inhibitory. J. Korean Soc. Agric. Chem. Biotechnol. 42: 105-110 (1999)
  36. Maruyama S, Nakagomi K, Tomizuka N, Suzuki H. Angiotensin converting enzyme inhibitor derived from an enzymatic hydrolysate of casein. Agric. Biol. Chem. 49: 1405-1410 (1985) https://doi.org/10.1080/00021369.1985.10866901
  37. MFDS (Ministry of Food and Drug Safety). 2021 Korea food safety. Available from: https://www.foodsafetykorea.go.kr/portal/safefoodlife/foodMeterial/foodMeterialDB.do?menu_grp=MENU_NEW04&menu_no=2968. Accessed Nov. 11, 2021.
  38. Mo HY, Jung YH, Jeong JS, Choi KH, Choi SW, Park CS, Choi MA. Kim ML, Kim MS. Quality characteristics of vinegar fermented using Omija (Schizandra chinensis Baillon). J. Korean Soc. Food Sci. Nutr. 42: 441-449 (2013) https://doi.org/10.3746/JKFN.2013.42.3.441
  39. Nakanc S. Food useful for preventing alcohol intoxication containing persimmon-vinegar and optimum fruits, with blood alcohol concentration reducing action. Japan patent. 63: 562-566 (1988)
  40. OH YJ. A study on cultural conditions for acetic acid production employing Pear Juice. J. Korean Soc. Food Nutr. 21: 377-380 (1992)
  41. Park YH, Choi JH, Whang K, Lee SO, Yang SA, Yu MH. Inhibitory effects of lyophilized dropwort vinegar powder on adipocyte differentiation and inflammation. J. Life Sci. 24: 476-484 (2014) https://doi.org/10.5352/JLS.2014.24.5.476
  42. Putri L, Katrin, Rissyelly, Putu GMWM. Inhibition activity of angiotensin converting enzyme (ACE) and determination of total phenolic and flavonoid compound from Bitter Melon Leaves (Momordica charantia L.). Pharmacogn. J. 9: 252-256 (2017) https://doi.org/10.5530/pj.2017.2.43
  43. Sakanakaand S, Ishihara Y. Comparison of antioxidant properties of persimmon vinegar and some other commercial vinegars in radical-scavenging assays and on lipid oxidation in tuna homogenates. Food Chem. 107: 739-744 (2008) https://doi.org/10.1016/j.foodchem.2007.08.080
  44. Seo JH, Lee GD, Jeong YJ. Optimization of the vinegar fermentation using concentrated apple juice. J. Korean Soc. Food Sci. Nutr. 30: 460-465 (2001)
  45. Shin JY, Kang JR, Shin JH, Seo WT, Byun HU, Choi JS, Kang MJ. Effects of Seomaeyakssuk (Artemisia argyi H.) vinegar on lipid metabolism in rats fed a high-fat and high-cholesterol diet. J. Korean Soc. Food Sci. Nutr. 46: 779-789 (2017) https://doi.org/10.3746/JKFN.2017.46.7.779
  46. Shinde J, Taldone T, Barletta M, Kunaparaju N, Hu B, Kumar S, Placido J, William ZS. α-glucosidase inhibitory activity of Syaygium cumini (Linn.) skeels seed kernel in vitro and in Goto-Kakizaki (GK) rats. Carbohyd. Res. 343: 1278-1281 (2008) https://doi.org/10.1016/j.carres.2008.03.003
  47. Shin DH, Kim DH, Choi U, Lim MS, An EY. Effect of red pepper varieties on the microflora, enzyme activities and taste components of traditional Kochujang during fermentation. J. Korean Soc. Food Sci. Nutr. 26: 1050-1057 (1997)
  48. Vogel RA. Corretti MC. Plotnick GD. The postprandial effect of components of the mediterranean diet on endothelial function. J. AM. Coll. Cardiol. 36: 1455-1460 (2000) https://doi.org/10.1016/S0735-1097(00)00896-2
  49. Watanabe J, Kawabata J, Kurihara H, NiKi R. Isolation and identification of α-glucosidase inhibitors from tochucha (Eucommia ulmoides). Biosci. Biotech. Biochem. 61: 177-178 (1997) https://doi.org/10.1271/bbb.61.177
  50. Yoon HN. Simultaneous gas chromatographic analysis of ethanol and acetic acid in vinegar. Korean J. Food Sci. Technol. 30: 1247-1251 (1998)
  51. Yoon HN. Chemical characterization of commercial vinegars. Korean J. Food Sci. Technol. 31: 1440-1446 (1999)