Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Validation and measurement uncertainty of HPLC-UV method for quercetin quantification in various foods

  • Seo, Eunbin (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Lim, Suji (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Yun, Choong-In (Lab of Nanobio, Seoul National University of Science and Technology) ;
  • Shin, Jae-Wook (Korea Advanced Food Research Institute) ;
  • Kim, Young-Jun (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • Received : 2021.09.15
  • Accepted : 2021.10.05
  • Published : 2021.12.31
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Abstract

The purpose of this study was to validate a high-performance liquid chromatography (HPLC) method for the quantitative analysis of quercetin in various foods. The method was based on HPLC-UV (360 nm). The method was validated using candy, beverage, and sausage which were tested for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy, and the measurement uncertainty was assessed. Matrix-matched calibration was also applied. The calibration curves (0.5-50 mg/L) showed good linearity (r2≥0.9998). LOD and LOQ ranged from 0.15 to 0.31 mg/kg and from 0.44 to 0.93 mg/kg, respectively. The average accuracy and precision at 0.5, 2.5, and 10 mg/kg ranged from 84.3 to 102.0% and 0.7 to 3.0 relative standard deviation (RSD%), respectively. This study confirmed the applicability of the proposed method by applying it to commercial products, such as teas and beverages. Thus, the proposed analytical method is suitable for quantifying quercetin in various foods.

Keywords

Acknowledgement

This research was supported by a grant (19162MFDS012) from Ministry of Food and Drug Safety in 2021.

References

  1. Abdelkawy KS, Balyshev ME, Elbarbry F. A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats. Biomed. Chromatogr. 31: e3819 (2017) https://doi.org/10.1002/bmc.3819
  2. Aguirre-Hernandez E, Gonzalez-Trujano ME, Martinez AL, Moreno J, Kite G, Terrazas T, Soto-Hernandez M. HPLC/MS analysis and anxiolytic-like effect of quercetin and kaempferol flavonoids from Tilia americana var. mexicana. J. Ethnopharmacol. 127: 91-97 (2010) https://doi.org/10.1016/j.jep.2009.09.044
  3. Albishi T, John JA, Al-Khalifa AS, Shahidi F. Antioxidative phenolic constituents of skins of onion varieties and their activities. J. Funct. Foods. 5: 1191-1203 (2013) https://doi.org/10.1016/j.jff.2013.04.002
  4. AOAC. Official methods of analysis of AOAC International. AOAC International, Rockville, MD, USA. (2016)
  5. Aybastier O, Sahin S, Demir C. Response Surface Optimized Ultra-sonic-Assisted Extraction of Quercetin and Isolation of Phenolic Compounds FromHypericum perforatumL. by Column Chromatography. Sep. Sci. Technol. 48: 1665-1674 (2013) https://doi.org/10.1080/01496395.2012.760603
  6. Buiarelli F, Bernardini F, Di Filippo P, Riccardi C, Pomata D, Simonetti G, Risoluti R. Extraction, Purification, and Determination by HPLC of Quercetin in Some Italian Wines. Food Anal. Method. 11: 3558-3562 (2018) https://doi.org/10.1007/s12161-018-1337-4
  7. Chen Y, Yu H, Wu H, Pan Y, Wang K, Jin Y, Zhang C. Characterization and quantification by LC-MS/MS of the chemical components of the heating products of the flavonoids extract in pollen typhae for transformation rule exploration. Molecules 20: 18352-18366 (2015) https://doi.org/10.3390/molecules201018352
  8. CODEX Alimentarius Commission. Guidance on Measurement Uncertainty and Uncertainty of Sampling Guidance on Measurement Uncertainty, CX/MAS 08/29/9. Joint FAO/WHO Food Standards Programme CODEX Committee on Methods of Analysis and Sampling. Budapest, Hungary. (2008)
  9. Dai Y, Row KH. Application of natural deep eutectic solvents in the extraction of quercetin from vegetables. Molecules 24: 2300 (2019) https://doi.org/10.3390/molecules24122300
  10. Davis JM, Murphy EA, Carmichael MD. Effects of the dietary flavonoid quercetin upon performance and health. Curr. Sport. Med. Rep. 8: 206-213 (2009) https://doi.org/10.1249/JSR.0b013e3181ae8959
  11. de Souza Dias F, Silva MF, David JM. Determination of quercetin, gallic acid, resveratrol, catechin and malvidin in Brazilian wines elaborated in the Vale do Sao Francisco using liquid-liquid extraction assisted by ultrasound and GC-MS. Food Anal. Method. 6: 963-968 (2012) https://doi.org/10.1007/s12161-012-9507-2
  12. Du FY, Xiao XH, Luo XJ, Li GK. Application of ionic liquids in the microwave-assisted extraction of polyphenolic compounds from medicinal plants. Talanta 78: 1177-1184 (2009) https://doi.org/10.1016/j.talanta.2009.01.040
  13. Egert S, Wolffram S, Schulze B, Langguth P, Hubbermann EM, Schwarz K, Adolphi B, Bosy-Westphal A, Rimbach G, Muller MJ. Enriched cereal bars are more effective in increasing plasma quercetin compared with quercetin from powder-filled hard capsules. Brit. J. Nutr. 107: 539-546 (2012) https://doi.org/10.1017/S0007114511003242
  14. Ellison SL, Williams A. Quantifying Uncertainty in Analytical Measurements, EURACHEM/CITAC, London, UK, pp. 33-89 (2012)
  15. Ferry DR, Smith A, Malkhandi J, Fyfe DW, deTakats PG, Anderson D, Baker J, Kerr DJ. Phase I clinical trial of the flavonoid quercetin: pharmacokinetics and evidence for in vivo tyrosine kinase inhibition. Clin. Cancer Res. 2: 659-668 (1996)
  16. Food and Drug Administration. Methods, Method Verification and Validation. (2014)
  17. Gao L, Liu G, Wang X, Liu F, Xu Y, Ma J. Preparation of a chemically stable quercetin formulation using nanosuspension technology. Int. J. Pharm. 404: 231-237 (2011) https://doi.org/10.1016/j.ijpharm.2010.11.009
  18. Glavac NK, Stojilkovski K, Kreft S, Park CH, Kreft I. Determination of fagopyrins, rutin, and quercetin in Tartary buckwheat products. LWT-Food Sci. Technol. 79: 423-427 (2017) https://doi.org/10.1016/j1wt.2017.01.068
  19. Gonzalez-Gonzalez RM, Barragan-Mendoza L, Peraza-Campos AL, Muniz-Valencia R, Ceballos-Magana SG, Parra-Delgado H. Validation of an HPLC-DAD method for the determination of plant phenolics. Rev. Bras. Farmacogn. 29: 689-693 (2019) https://doi.org/10.1016/j.bjp.2019.06.002
  20. ISO/IEC 17025, General requirements for the competence of testing and calibration laboratories. International Organization for Standardization/International Electrotechnical Committee, Geneva (2017)
  21. JECFA, Evaluation of certain food additives. In: Proceedings of 21st JECFA session, WHO technical report series, no. 617, 18-27, Geneva, Switzer land (1977)
  22. Kwak JH, Seo JM, Kim NH, Arasu MV, Kim S, Yoon MK, Kim SJ. Variation of quercetin glycoside derivatives in three onion (Allium cepa L.) varieties. Saudi. J. Biol. Sci. 24: 1387-1391 (2017) https://doi.org/10.1016/j.sjbs.2016.05.014
  23. Lai WF, Wong WT. Design and optimization of quercetin-based functional foods. Crit. Rev. Food Sci. Nutr. 1-17 (2021)
  24. Li Y, Yao J, Han C, Yang J, Chaudhry MT, Wang S, Liu H, Yin Y. Quercetin, inflammation and immunity. Nutrients 8: 167 (2016) https://doi.org/10.3390/nu8030167
  25. Lin J, Gwyneth Tan YX, Leong LP, Zhou W. Steamed bread enriched with quercetin as an antiglycative food product: its quality attributes and antioxidant properties. Food Funct. 9: 3398-3407 (2018) https://doi.org/10.1039/c8fo00818c
  26. Molinelli A, Weiss R, Mizaikoff B. Advanced solid phase extraction using molecularly imprinted polymers for the determination of quercetin in red wine. J. Agri. Food Chem. 50: 1804-1808 (2002) https://doi.org/10.1021/jf011213q
  27. Prakash D, Singh BN, Upadhyay G. Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa). Food Chem. 102: 1389-1393 (2007) https://doi.org/10.1016/j.foodchem.2006.06.063
  28. Prikryl J, Hajek T, Svecova B, Salek RN, Cernikova M, Cervenka L, Bunka F. Antioxidant properties and textural characteristics of processed cheese spreads enriched with rutin or quercetin: The effect of processing conditions. Lwt 87: 266-271 (2018) https://doi.org/10.1016/j.lwt.2017.08.093
  29. Priprem A, Watanatorn J, Sutthiparinyanont S, Phachonpai W, Muchimapura S. Anxiety and cognitive effects of quercetin liposomes in rats. Nanomed-nanotechnol. 4: 70-78 (2008) https://doi.org/10.1016/j.nano.2007.12.001
  30. Sante E. Guidance document on analytical quality control and method validation procedures for pesticides residues analysis in food and feed. European Commission SANTE/11813/2017, 1-46 (2015)
  31. Sharifi N, Mahernia S, Amanlou M. Comparison of different methods in quercetin extraction from leaves of Raphanus sativus L. Pharm. Sci. 23: 59-65 (2016) https://doi.org/10.15171/ps.2017.09
  32. Sharifuldin M, Ismail Z, Aisha A, Seow E, Beh H. Quantification of rutin, quercitrin and quercetin in Cosmos caudatus Kunth by reverse phase high performance liquid chromatography. Qual. Assur. Saf. Crop. 8: 617-622 (2016) https://doi.org/10.3920/QAS2015.0839
  33. Tayade AB, Dhar P, Sharma M, Chauhan RS, Chaurasia OP, Srivastava RB. Antioxidant capacities, phenolic contents, and GC/MS analysis of Rhodiola imbricata Edgew. root extracts from Trans-Himalaya. J. Food Sci. 78: C402-410 (2013) https://doi.org/10.1111/1750-3841.12054
  34. The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH). ICH Harmonised Tripartite Guideline. Q2(R1): Validation of analytical procedures: Text and methodology. Geneva, Switzerland (2005)
  35. Tokusoglu O, unal M, Yildirim Z. HPLC-UV and GC-MS characterization of the flavonol aglycons quercetin, kaempferol, and myricetin in tomato pastes and other tomato-based products. Acta. Chromatogr. 13: 196-207 (2003)
  36. Vasantha Rupasinghe HP, Kathirvel P, Huber GM. Ultra-sonication-assisted solvent extraction of quercetin glycosides from 'Idared' apple peels. Molecules 16: 9783-9791 (2011) https://doi.org/10.3390/molecules16129783
  37. Wach A, Pyrzynska K, Biesaga M. Quercetin content in some food and herbal samples. Food Chem. 100: 699-704 (2007) https://doi.org/10.1016/j.foodchem.2005.10.028