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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Investigation of the effect of Staufen1 overexpression on the HIV-1 virus production

  • Park, Seong-won (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea) ;
  • Yu, Kyung-Lee (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea) ;
  • Bae, Jun-Hyun (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea) ;
  • Kim, Ga-Na (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea) ;
  • Kim, Hae-In (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea) ;
  • You, Ji Chang (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
  • Received : 2021.06.03
  • Accepted : 2021.07.07
  • Published : 2021.11.30
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Abstract

In this study, we investigated how Staufen1 influences the HIV-1 production. The overexpression of Staufen1 increased virus production without any negative affect on the viral infectivity. This increase was not caused by transcriptional activation; but by influencing post-transcriptional steps. Using multiple Gag protein derivatives, we confirmed that the zinc-finger domains of the HIV-1 nucleocapsid (NC) are important for its interaction with Staufen1. We also found that Staufen1 colocalized in stress granules with the mature form of the HIV-1 NC protein.

Keywords

Acknowledgement

This work was supported by a research foundation of Korea (NRF) Grant funded by the Korean Government (2020R1F1A1075725 and 2017R1A5A1015366).

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