Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography

  • Kim, Youngkyu (Asan Institute for Life Sciences, Asan Medical Center) ;
  • Kim, Tae-Keun (Asan Institute for Life Sciences, Asan Medical Center) ;
  • Shin, Yeonhee (Department of Convergence Medicine, University of Ulsan College of Medicine) ;
  • Tak, Eunyoung (Asan Institute for Life Sciences, Asan Medical Center) ;
  • Song, Gi-Won (Division of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Oh, Yeon-Mok (Department of Pulmonary and Critical Care Medicine, Clinical Research Center for Chronic Obstructive Airway Diseases, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Kim, Jun Ki (Asan Institute for Life Sciences, Asan Medical Center) ;
  • Pack, Chan-Gi (Asan Institute for Life Sciences, Asan Medical Center)
  • Received : 2021.07.16
  • Accepted : 2021.09.09
  • Published : 2021.11.30
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Abstract

Label-free optical diffraction tomography (ODT), an imaging technology that does not require fluorescent labeling or other pre-processing, can overcome the limitations of conventional cell imaging technologies, such as fluorescence and electron microscopy. In this study, we used ODT to characterize the cellular organelles of three different stem cells-namely, human liver derived stem cell, human umbilical cord matrix derived mesenchymal stem cell, and human induced pluripotent stem cell-based on their refractive index and volume of organelles. The physical property of each stem cell was compared with that of fibroblast. Based on our findings, the characteristic physical properties of specific stem cells can be quantitatively distinguished based on their refractive index and volume of cellular organelles. Altogether, the method employed herein could aid in the distinction of living stem cells from normal cells without the use of fluorescence or specific biomarkers.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) through the Korean Ministry of Science & ICT (MSIT) (grant Nos., 2018R1D1A1B07048696, 2018R1A5A2020732, and 2019R1A2C2084122); the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant No. HI18C2391); and a grant from the Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea (grant No. 2021IP0035-1). We would like to thank Tomocube Inc. for their technical support and discussion.

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