한국유기농업학회지 (Korean Journal of Organic Agriculture)

  • 제29권4호
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  • Pages.613-623
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  • 2021
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  • 1229-3571(pISSN)
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  • 2287-819X(eISSN)
한국유기농업학회 (Korean Association of Organic Agriculture)
권호 표지
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폴리페놀을 다량 함유한 커피박 및 녹차박 추출물의 수준별 첨가가 반추위 발효 및 메탄 발생량에 미치는 영향

Effects of Addition Levels of Coffee and Green Tea By-products Extract including Polyphenols on in vitro Rumen Fermentation and Methane Emission

  • 원미영 (국립농업과학원 농업미생물과) ;
  • 류채화 (국립축산과학원 동물영양생리과) ;
  • 박혜련 (전북대학교 축산학과) ;
  • 채병호 (전북대학교 축산학과) ;
  • 장승호 (전북대학교 축산학과) ;
  • 최승신 (한국사료협회) ;
  • 최봉환 (국립축산과학원 가축유전자원센터) ;
  • 이성수 (국립축산과학원 가축유전자원센터) ;
  • 이진욱 (국립축산과학원 가축유전자원센터) ;
  • 최낙진 (전북대학교 동물자원과학과)
  • 투고 : 2021.11.02
  • 심사 : 2021.11.22
  • 발행 : 2021.11.30
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초록

This study was conducted to investigate the effect of addition levels of coffee and green tea by products extract including polyphenols through hot water extraction on rumen fermentation. The treatment groups consisted of coffee extract (CO), green tea extract (GR) and mixed extract (MIX), and the addition level was 10 µL, 20 µL and 30 µL of three levels. The experiment consisted of a total of 10 experimental groups including the control group, and a full factorial design was used. The effect of polyphenol addition in coffee and green tea by-products was analyzed through main and interaction effect of statistical analysis. The total polyphenol content of the extracts was 106.15, 79.10 and 185.25 ㎍ GAE/g DM for coffee by-product, green tea by-product and mixture, respectively. Total gas production was significantly lower in the treatment groups than in the control (114.00 mL/gDM) (p<0.05). Methane emission tended to decrease as the polyphenol addition level increased. Moreover, the MIX showed the lowest methane emission when 30 µL was added (p<0.05). Volatile fatty acids showed a significant difference compared to the treatment group as a control (98.06 mM) (p<0.05), but there was no change according to the level of polyphenols. As a result of the main effect and interaction, it is thought that the effect on methane reduction and improvement of rumen fermentation in MIX20 can be expected. In a series of studies, the addition of 20 µL of a blended extract of coffee and green tea by-products is thought to reduce methane to levels that do not inhibit rumen fermentation.

키워드

과제정보

본 연구는 반추가축용 발효사료 제조 기술 확립 및 효과 구명(PJ01564702)의 지원과 2021년도 농촌진흥청 국립축산과학원 전문연구원 과정 지원 사업에 의해 이루어진 것임.

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