한국유기농업학회지 (Korean Journal of Organic Agriculture)

  • 제29권4호
  • /
  • Pages.575-588
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  • 2021
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  • 1229-3571(pISSN)
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  • 2287-819X(eISSN)
한국유기농업학회 (Korean Association of Organic Agriculture)
권호 표지
DOI QR코드

DOI QR Code

오이 탄저병원균과 잿빛곰팡이병원균에 억제 효과가 있는 방선균 선발

Selection of Antagonistic Soil Actinomycetes Against Both Colletotrichum orbiculare and Botrytis cinerea in Cucumber Plants

  • 곽화숙 (국립농업과학원 농업미생물과) ;
  • 김지원 (국립농업과학원 농업미생물과, 전북대학교 농생물학과) ;
  • 박진우 (국립농업과학원 농업미생물과, 농촌진흥청 대변인실) ;
  • 상미경 (국립농업과학원 농업미생물과)
  • 투고 : 2021.07.23
  • 심사 : 2021.09.30
  • 발행 : 2021.11.30
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초록

본 연구에서는 오이 지상부에 발생하는 두 가지 병원균 B. cinerea와 C. orbiculare에 항균활성을 가지는 유용한 방선균을 선발하고자 하였다. 토양에서 분리한 방선균 560 균주 중 두 병원균에 대해 동시 항균활성을 나타낸 7균주를 일차적으로 선발한 후, 배양여액의 항균활성 검정을 위한 대치배양과 오이 식물체 검정을 통해 최종적으로 S20-465 균주를 선발하였다. 이 균주는 염기서열 비교 분석을 통해 Streptomyces sp.로 동정하였다. S20-465 균주의 배양여액은 두 병원균의 균사 생장을 60%이상 억제하였다. 배양여액의 20배, 40배 희석액을 분무 처리할 경우 오이 잎에서의 잿빛곰팡이병에 의한 병반길이를 감소시켰을 뿐만 아니라 탄저병의 발병도도 대조구에 비해 50% 이상 감소시켜 생물검정에서도 병억제 효과가 있는 것을 확인하였다. 따라서 최종 선발한 S20-465는 앞으로 생물방제 미생물소재로써 활용할 수 있을 것으로 사료된다.

The purpose of this study is to select actinomycetes with antifungal activity against Botrytis cinerea and Colletotrichum orbiculare, which are airborne pathogens in cucumber plants. In 2020, 560 species of actinomycetes were isolated from rhizome soils of various vegetables in Korea; 7 strains showing simultaneous antifungal activity against two pathogens were selected. Finally, strain S20-465 was selected through dual culture and plant assay. This strain was identified as Streptomyces sp. based on 16S rRNA analysis. The culture filtrate of strain S20-465 inhibited mycelial growth of both pathogens by more than 60%. In addition, when cucumber plants were treated with 20-fold and 40-fold diluted culture filtrates of S20-465, lesions caused by B. cinerea and C. orbiculare on cucumber leaves were significantly reduced compared to the control. This results suggest that strain S20-465 produces specific secondary metabolites with antifungal activity against both pathogens.

키워드

과제정보

본 연구는 국립농업과학원 연구개발사업(Project No : PJ014978)의 지원에 의하여 이루어짐.

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