대한한의학방제학회지 (Herbal Formula Science)

  • 제28권4호
  • /
  • Pages.339-349
  • /
  • 2020
  • /
  • 1229-1218(pISSN)
  • /
  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
권호 표지
DOI QR코드

DOI QR Code

위령탕 혼합단미연조엑스의 항염증 효과

Anti-inflammatory Effects of Wiryeong-tang Mix Soft Extract

  • Kim, Myoung-Jin (National Institute for Korean Medicine Development) ;
  • Kim, Se-Jin (National Institute for Korean Medicine Development) ;
  • Nam, Won-Hee (National Institute for Korean Medicine Development) ;
  • Leem, Hyun-Hee (National Institute for Korean Medicine Development) ;
  • Jeon, Young-Hee (National Institute for Korean Medicine Development) ;
  • Son, Su-Mi (National Institute for Korean Medicine Development) ;
  • Kim, Jung-Ok (National Institute for Korean Medicine Development)
  • 투고 : 2020.11.12
  • 심사 : 2020.11.27
  • 발행 : 2020.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : Wiryeong-tang (WRT) is a traditional herbal medicine used to treat kidney-related diseases. However, the anti-inflammatory and anti-gastritis effect of Wiryeong-tang was not well known. Therefore, we experimented to confirmed the anti-inflammatory and anti-gastritis effects of Wiryeong-tang. Methods : The RAW 264.7 cells were pre treated with Wiryeong-tang mix soft extract (WRT-mse; 50, 100 and 200 ㎍/mL) for 1 hrs, and then incubated with lipopolysaccharide (LPS; 500 ng/mL). Cell viability was measured by the MTT method, and nitric oxide (NO) was measured with griess reagent. In addition, pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). For anti-gastritis effect in vivo, acute gastritis was induced using 150 mM HCl/60% ethanol used ICR mice. WRT-mse (133 mg/kg) was pre treated for 3 days and then treated with 150 mM HCl/60% ethanol 1 hrs later. Then gastritis was observed and inflammatory cytokines in the gastric tissue was measured. Results : The 8 marker components of the WRT-mse were determined by simultaneous analysis using HPLC. WRT-mse was not toxic and inhibited pro-inflammatory cytokines such as IL-1β, IL-6 and TNF-α at NO production, protein and mRNA levels. Also, it was confirmed that WRT-mse improved bleeding and edema in gastritis, and suppresses inflammatory cytokines. Conclusion : In summary, our results suggest that the treatment of the WRT-mse reduced and improved the 150 mM HCl/60% ethanol induced acute gastritis and the inflammation caused by LPS stimulation in RAW 264.7 cells. Therefore, this study may provide useful drug or clinical evidence for WRT-mse to prevent inflammation.

키워드

참고문헌

  1. Kim MR, Kang OH, Kong R, Seo YS, Zhou T, Kim SA, Kim ES, et al. The study of anti-inflammatory effect of Hyeonto-dan extract in RAW 264.7 macrophage. Kor. J. Herbology. 2017 ; 32(2) : 77-85.
  2. Laroux FS. Mechanisms of inflammation : the good, the bad and the ugly. Front. Biosci. 2004;9:3156-3162. https://doi.org/10.2741/1468
  3. Behrens EM. Macrophage activation syndrome in rheumatic disease: what is the role of the antigen presenting cell?. Autoimmun Rev. 2008;7(4):305-308. https://doi.org/10.1016/j.autrev.2007.11.025
  4. Kim MJ, Bae GS, Choi SB, Jo IJ, Kim DG, Shin JY, et al. The anti-inflammatory effect of Taraxacum coreanum on lipopolysaccharide induced inflammatory response on RAW 264.7 cells. Kor. J. Herbology. 2014 ; 29(6) : 21-26. https://doi.org/10.6116/kjh.2014.29.6.21.
  5. Kuzmich NN, Sivak KV, Chubarev VN, Porozov YB, Savateeva-Lyubimova TN, Peri F. TLR4 signaling pathway modulators as potential therapeutics in inflammation and sepsis. Vaccines. 2017;5(4):34. https://doi.org/10.3390/vaccines5040034
  6. Jung JY, Byun SH, Park CA, Cho IJ, Kim SC. Anti-inflammatory effects of Epimedii Herba water extract through inhibition of nuclear factor-κB in RAW 264.7 Cells. Kor. J. Herbology. 2018 ; 33(2) : 19-28.
  7. Kim DH, Park SJ, Jung JY, Kim SC, Byun SH. Antiinflammatory effects of the aqueous extract of Hwangnyenhaedok-tang in LPS- activated macrophage cells. Kor J Herbology. 2009 ; 24(4):39-47.
  8. Choi SB, Bae GS, Jo IJ, Park KC, Seo SH, Kim DG, et al. The anti-inflammatory effect of Lithospermum Erythrorhizon on lipopolysaccharide - induced inflammatory response in RAW 264.7 cells. Kor. J. Herbology. 2013 ; 28(2) : 67-73. https://doi.org/10.6116/kjh.2013.28.2.67
  9. McDaniel ML, Kwon G, Hill JR, Marshall CA, Corbett JA. Cytokines and nitric oxide in islet inflammation and diabetes. Proc Soc Exp Biol Med. 1996 ; 211(1):24-32. https://doi.org/10.3181/00379727-211-43950D
  10. Hwang DH, Kang MJ, Jo MJ, Seo YB, Park NG, Kim GD. Anti-inflammatory activity of β-thymosin peptide derived from Pacific oyster (Crassostrea gigas) on NO and PGE2 production by down-regulating NF-κB in LPS-induced RAW264.7 macrophage cells. Mar Drugs. 2019;17(2):129. https://doi.org/10.3390/md17020129
  11. Kim HJ, Han JS, Kim SH, Park HJ, Park HS, Ko MK et al. Study on the efects of natural herb extract against acute gastritis induced by HCl-ethanol in SD rats. Journal of Animal Asisted Psychotherapy. 2017;6(1):55-67.
  12. Kim OK, Nam DE, You YH, Jun WJ, Le JM. Protective effect of Canavalia gladiata on gastric inflammation induced by alcohol treatment in rats. J Korean Soc Fod Sci Nutr. 2013;42(5):690-696. https://doi.org/10.3746/jkfn.2013.42.5.690
  13. Lee JJ, Choi HS, Lee JH, Jung CJ, Lee MY. The effects of ethylacetate fraction of Sanguisorba officinalis L. on Experimentally-induced acute gastritis and peptic ulcers in rats. J Korean Soc Fod Sci Nutr. 2005; 34(10):1545-1552. https://doi.org/10.3746/jkfn.2005.34.10.1545
  14. Lee AR, Lee JY, Kim MY, Shin MR, Shin SH, Seo B, et al. Protective effects of a Lycium chinense ethanol extract through anti-oxidative stress on acute gastric lesion mice. Kor J Herbology. 2015 ; 30(6) : 63-68. https://doi.org/10.6116/kjh.2015.30.6.63.
  15. Jeong CS, Hyun JE, Kim YS. Anti - oxidative effect of Ginsenoside Rb1 on the HCl, ethanol - induced gastric tissue in rats. Kor. J. Pharmacogn. 2002;33(3):252-256.
  16. Kim MG, Lee YJ, Choi ES, Yoon JJ, Han BH, Kang DG, et al. Wiryeongtang regulates hypertonicity-induced expression of aquaporin-2 water channels in mIMCD-3 cells. MOLECULAR MEDICINE REPORTS. 2017;15(5):2665-2672. https://doi.org/10.3892/mmr.2017.6296
  17. Yoon JJ, Han BH, Choi ES, NamGung S, Jeong DH, Kin HY, et al. Wiryeongtang atenuates diabetic renal dysfunction in human renal mesangial cels. Kor J Herbology. 2016 ; 31(5) : 71-78. https://doi.org/10.6116/kjh.2016.31.5.71.
  18. Yun GY. Oriental herbal formula science. Seoul, Publisher myeongbo. 1985;198-204.
  19. Jang HJ, Shin GC, Le WC. Efect of Wiryeongtang on triton WR-139-induced hyperlipidemia rat. J of Oriental Chr Dis. 1988;4(1):98-121.
  20. Kwqamata H, Ochiai H, Mantani N, terasawa K. Enhanced expression of inducible nitric oxied RAW264.7 cells, a murine macrophage cell line. Am J Chin Med. 2000;28(02):217-226. https://doi.org/10.1142/S0192415X0000026X
  21. Chiou WF, Chou CJ, Chen CF. Camptothecin suppresses nitric oxied biosythesis in RAW264.7 macrophages. Life Sci. 2001;69(6):625-635. https://doi.org/10.1016/S0024-3205(01)01154-7
  22. Kim MS, Jeong JS, Lee HY, Ju YS, Bae GS, Seo SW, et al. The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages. Kor. J. Herbology. 2011;26(2):51-57.
  23. Lee JA, Kim SH, Kim MJ, Ahn JH, Park HJ, Lee WR, et al. Protective effects of Chrysanthemi indici Flos extract and Flaxsed oil mixture on HCl/ethanol-induced Acute Gastric Lesion Mice. Kor J Herbology. 2018 ; 33(6) : 19-28. https://doi.org/10.6116/KJH.2018.33.6.19
  24. Park IJ, Yun SH, Choi GH, Kim HJ, Seo YH, Cho JH. Protective effect of Litsea japonica fruit flesh extract on stress- induced gastritis in rats. J. Food Hyg. Saf. 2017;32(6):536-541. https://doi.org/10.13103/JFHS.2017.32.6.536
  25. Hong SH, Lee HA, Lee YS, Kim DW, Oh GW, Woo JW, et al. Protective effect of halophyte Salsola komarovi ljin against gastric ulcer Induce by alcohol treatment in rats. J Biomed Res. 2014;15(4):170-175. https://doi.org/10.12729/jbr.2014.15.4.170