Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Isolation of New Strain of Cordyceps militaris HB8 and Optimal Condition for Production of Adenosine and Cordycepin in Fruit Body

  • Li, Jin Feng (Department of Oriental Medicinal Biotechnology, Kyung Hee University) ;
  • Hoang, Van An (HanbangBio Inc. Kyung Hee University Holdings) ;
  • Ahn, Jong Chan (Graduate School of Biotechnology, Kyung Hee University) ;
  • Yang, Dong Uk (HanbangBio Inc. Kyung Hee University Holdings) ;
  • Lee, Dong Wook (HanbangBio Inc. Kyung Hee University Holdings) ;
  • Yang, Deok Chun (HanbangBio Inc. Kyung Hee University Holdings)
  • Received : 2020.11.03
  • Accepted : 2020.11.26
  • Published : 2020.12.01
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Abstract

Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the new Cordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militaris JLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8 was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession number MT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 mg/g (200 g of pupa, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 mg of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production of adenosine was 2.6 mg/g (15 g of skim milk powder, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 mg of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

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References

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