Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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High-Throughput In Vitro Screening of Changed Algal Community Structure Using the PhotoBiobox

  • Cho, Dae-Hyun (Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Cho, Kichul (Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea) ;
  • Heo, Jina (Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Kim, Urim (Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Lee, Yong Jae (Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Choi, Dong-Yun (Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Yoo, Chan (Department of Chemical and Biomolecular Engineering, KAIST) ;
  • Kim, Hee-Sik (Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Bae, Seunghee (Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University)
  • Received : 2020.06.18
  • Accepted : 2020.08.19
  • Published : 2020.11.28
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Abstract

In a previous study, the sequential optimization and regulation of environmental parameters using the PhotoBiobox were demonstrated with high-throughput screening tests. In this study, we estimated changes in the biovolume-based composition of a polyculture built in vitro and composed of three algal strains: Chlorella sp., Scenedesmus sp., and Parachlorella sp. We performed this work using the PhotoBiobox under different temperatures (10-36℃) and light intensities (50-700 μmol m-2 s-1) in air and in 5% CO2. In 5% CO2, Chlorella sp. exhibited better adaptation to high temperatures than in air conditions. Pearson's correlation analysis showed that the composition of Parachlorella sp. was highly related to temperature whereas Chlorella sp. and Scenedesmus sp. showed negative correlations in both air and 5% CO2. Furthermore, light intensity slightly affected the composition of Scenedesmus sp., whereas no significant effect was observed in other species. Based on these results, it is speculated that temperature is an important factor in influencing changes in algal polyculture community structure (PCS). These results further confirm that the PhotoBiobox is a convenient and available tool for performance of lab-scale experiments on PCS changes. The application of the PhotoBiobox in PCS studies will provide new insight into polyculture-based ecology.

Keywords

References

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