The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Gyejigamchoyonggolmoryeo-tang on sleep latency and total duration of sleep induced by thiopental sodium in mice

계지감초용골모려탕(桂枝甘草龍骨牡蠣湯)이 치오펜탈 나트륨으로 유도된 수면의 입면 및 수면 시간에 미치는 영향

  • Received : 2020.10.08
  • Accepted : 2020.11.25
  • Published : 2020.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Gyejigamchoyonggolmoryeo-tang (GT, Guizhigancaolonggumulitang in Chinese) is a herbal medicine to be prescribed for insomnia caused by anxiety induced by Heart-Heat and elevated Liver-Yang. In the present study, the onset time (sleep latency) and the duration time of sleep were measured to find out the sleep inducing effects of GT. The expression of c-fos immunohistochemistry was also measured at the ventrolateral preoptic area (VLPO) and tuberomammillary nucleus (TMN) site in brain. Methods : The onset time (sleep latency) and the duration time of sleep were measured 30 minutes after thiopental sodium injection. Thereafter, brain tissue was obtained and c-fos immunohistochemistry was performed on the VLPO and TMN sites. Results : GT statistically significantly reduced the sleep latency required to enter sleep, and significantly increased sleep duration time. GT significantly increased the number of c-fos immunohistochemical staining-positive cells in the sleep-inducing center (VLPO), whereas GT significantly decreased the number of c-fos immunohistochemical staining-positive cells in the arousal center (TMN). Conclusions : It could be concluded that the GT shortened the sleep latency and increased the duration time for sleep induced by thiopental sodium. And it was confirmed that the mechanism was to stimulate the sleep-inducing center (VLPO) and suppress the arousal center (TMN) in the brain. The results of this study are considered to be useful as scientific evidence that can be used clinically for the treatment of insomnia caused by anxiety.

Keywords

References

  1. Jonathan RLS, Thomas R. Neurophysiology of Sleep and Wakefulness: Basic Science and Clinical Implications. Curr Neuropharmacol. 2008 ; 6(4) : 367-78. https://doi.org/10.2174/157015908787386050
  2. Zeitzer JM, Dijk DJ, Kronauer R, Brown E, Czeisler C. Sensitivity of the human circadian pacemaker to nocturnal light: melatonin phase resetting and suppression. J Physiol. 2000 ; 526(3) : 695-702. https://doi.org/10.1111/j.1469-7793.2000.00695.x
  3. Moon JJ, An GS, Kim JB, Park WS, Park JH, Kim DH et al. Precise Interpretation of 'Treatise on Cold Damage Diseases'. Yongin : Hanuimunhwa-sa. 2017 : 262.
  4. Zou J, Liang SH, Zhao CH, Ni LQ, Cao BY, Chen HJ, Tong Y. The effect of two kinds of traditional Chinese herbal compound on the behavior and hypothalamus-pituitary-adrenal gland axis of the chronic psychological stress rats. Journal of Jinan University. Natural science & medicine edition. 2005 ; 26(4) : 530-4.
  5. Kao TC. Treatment of Hidrosis with Dysphoria by GuizhiGancaoLongguMuli Decoction: A Case Report. Taiwan Journal of Clinical Chinese Medicine. 2008 ; 14(3) : 240-2.
  6. Louie GL, Prokocimer PG, Nicholls EA, Maze M. Aminophylline shortens thiopental sleep-time and enhances noradrenergic neurotransmission in rats. Brain Res. 1986 ; 383(1-2) : 377-81. https://doi.org/10.1016/0006-8993(86)90044-2
  7. da Silva JC, Scorza FA, Nejm MB, Cavalheiro EA, Cukiert A. c-FOS expression after hippocampal deep brain stimulation in normal rats. Neuromodulation. 2014 ; 17(3) : 213-7. https://doi.org/10.1111/ner.12122
  8. Jeong HY, Kwon YJ, Park IJ, Kwon JT, Han BH, Lee SP. Effects of the Sanjoin on the Rat Brain. Korean Journal of Biological Psychiatry. 1997 ; 4(1) : 54-9.
  9. Park JM. Oriental Medical Dictionary. Seoul : Kkachigeulbang. 1997 : 76.
  10. Textbook compilation committee of department of herbology in Korean medicine academy association. Bonch-hak (Textbook of herbology). 4th ed. Seoul : Younglim-sa. 2020 : 156, 520, 545, 577.
  11. Shin YI, Kim SH. GuksiShanghanlun. Seoul : Jumin Publisher. 2006 : 152.
  12. World Health Organization. WHO Model List of Essential Medicines. 16th ed. Geneva : World Health Organization. 2009.
  13. World Health Organization. WHO Model List of Essential Medicines. 21st ed. Geneva : World Health Organization. 2019.
  14. Calcaterra NE, Barrow JC. Classics in chemical neuroscience: diazepam (valium). ACS Chem Neurosci. 2014 ; 5(4) : 253-60. https://doi.org/10.1021/cn5000056
  15. Lobo IA, Harris RA. GABAA receptors and alcohol. Pharmacol Biochem Behav. 2008 ; 90(1) : 90-4.Z https://doi.org/10.1016/j.pbb.2008.03.006
  16. Malenka RC, Nestler EJ, Hyman SE. "Chapter 6: Widely Projecting Systems: Monoamines, Acetylcholine, and Orexin". In Sydor A, Brown RY (eds.). Molecular Neuropharmacology: A Foundation for Clinical Neuroscience. 2nd ed. New York : McGraw-Hill Medical. 2009 : 175-6.