Journal of the Korean Society of Marine Environment & Safety (해양환경안전학회지)

The Korean Society of Marine Environment and safety (해양환경안전학회)
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Early Detection of Cochlodinium polykrikoides (Dinophyceae) Blooms in Namhaedo in 2019 Using Quantitative Real-Time PCR (qPCR)

Quantitative real-time PCR (qPCR)을 이용하여 2019년 남해도 해역에서 발생한 Cochlodinium polykrikoides (Dinophyceae) 적조의 조기검출

  • Park, Tae Gyu (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • Kim, Jin Joo (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • Song, Seon Young (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS))
  • 박태규 (국립수산과학원 남동해수산연구소) ;
  • 김진주 (국립수산과학원 남동해수산연구소) ;
  • 송선영 (국립수산과학원 남동해수산연구소)
  • Received : 2020.08.13
  • Accepted : 2020.10.28
  • Published : 2020.10.31
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Abstract

Quantitative real-time polymerase chain reaction (qPCR) was applied for the early detection of red tides in the coastal areas of South Gyeongsang in 2019. Cochlodinium polykrikoides (Dinophyceae) was detected at very low cell densities (0.0015~0.0058 cells mL-1) in early June, but its cell density increased by up to 0.163 cells mL-1 in mid-August. Higher cell densities were detected mainly in Namhaedo using both qPCR and microscopy (maximum 24 cells mL-1) in late-August. Accordingly, a red tide alert was issued on September 2 (maximum 200 cells mL-1) on this island. C. polykrikoides cell density in Namhaedo peaked on September 11 (12,000 cells mL-1). Our results indicate that C. polykrikoides was detected at very low cell density in Namhaedo prior to bloom, which occurred in the same area. Therefore, qPCR is a useful tool to detect even at very low cell densities of C. polykrikoides for early warning of blooms.

적조가 처음 시작되는 해역을 조기에 파악하기 위하여 Quantitative real-time PCR (qPCR)을 경남해역 적조현장에 활용하였다. 2019년 경남해역을 대상으로 Cochlodinium polykrikoides를 qPCR로 정량분석한 결과, 6월 초에 저밀도로(0.0015~0.0058 cells mL-1) 검출되기 시작하여 8월 중순에는 최대 0.163 cells mL-1 밀도로 증가하였고, 주로 남해도 주변에서 높게 검출되었다. 8월 말에는 현미경 검경으로 남해도 주변에서 높게 출현함이 확인되었고(최대 24 cells mL-1), 9월 2일에는 남해도에서 적조주의보가 발령되었고(최대 200 cells mL-1), 9월 1일에는 최대 12,000 cells mL-1까지 남해도 해역에서 발생하였다. 위 결과는 극미량의 C. polykrikoides이 적조발생 전에 남해도에서 검출되었고 이후 같은 해역에서 적조가 발생되었음을 보여준다. 이는 qPCR이 극미량의 C. polykrikoides을 조기검출하는데 유용한 방법임을 보여준다.

Keywords

References

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