Korean Journal of Exercise Nutrition (운동영양학회지)

한국운동영양학회
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Muscle oxygenation, endocrine and metabolic regulation during low-intensity endurance exercise with blood flow restriction

  • Hwang, Hyejung (Graduate school of Sport and Health Science, Ritsumeikan University) ;
  • Mizuno, Sahiro (Research Center of Health, Physical Fitness and Sports, Nagoya University) ;
  • Kasai, Nobukazu (Department of Sports Science, Japan Institute of Sports Sciences) ;
  • Kojima, Chihiro (Department of Sports Science, Japan Institute of Sports Sciences) ;
  • Sumi, Daichi (Research Center for Urban Health and Sports, Osaka City University) ;
  • Hayashi, Nanako (Research Center for Urban Health and Sports, Osaka City University) ;
  • Goto, Kazushige (Research Center for Urban Health and Sports, Osaka City University)
  • Received : 2020.06.13
  • Accepted : 2020.06.26
  • Published : 2020.06.30
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Abstract

[Purpose] The present study investigated the effect of endurance exercise with blood flow restriction (BFR) performed at either 25% maximal oxygen uptake (${\dot{V}}O_2$ max) or 40% ${\dot{V}}O_2$ max) on muscle oxygenation, energy metabolism, and endocrine responses. [Methods] Ten males were recruited in the present study. The subjects performed three trials: (1) endurance exercise at 40% ${\dot{V}}O_2$ max without BFR (NBFR40), (2) endurance exercise at 25% ${\dot{V}}O_2$ max with BFR (BFR25), and (3) endurance exercise at 40% ${\dot{V}}O_2$ max with BFR (BFR40). The exercises were performed for 15 min during which the pedaling frequency was set at 70 rpm. In BFR25 and BFR40, 2 min of pressure phase (equivalent to 160 mmHg) followed by 1 min of release phase were repeated five times (5 × 3 min) throughout 15 minutes of exercise. During exercise, muscle oxygenation and concentration of respiratory gases were measured. The blood samples were collected before exercise, immediately after 15 min of exercise, and at 15, 30, and 60 minutes after completion of exercise. [Results] Deoxygenated hemoglobin (deoxy-Hb) level during exercise was significantly higher with BFR25 and BFR40 than that with NBFR40. BFR40 showed significantly higher total-hemoglobin (total-Hb) than NBFR40 during 2 min of pressure phase. Moreover, exercise-induced lactate elevation and pH reduction were significantly augmented in BFR40, with concomitant increase in serum cortisol concentration after exercise. Carbohydrate (CHO) oxidation was significantly higher with BFR40 than that with NBFR40 and BFR25, whereas fat oxidation was lower with BFR40. [Conclusion] Deoxy-Hb and total Hb levels were significantly increased during 15 min of pedaling exercise in BFR25 and BFR40, indicating augmented local hypoxia and blood volume (blood perfusion) in the muscle. Moreover, low-and moderate-intensity exercise with BFR facilitated CHO oxidation.

Keywords

Acknowledgement

This work was supported by the Ministry of Education of the Republic of Korea and the National Research Foundation of Korea(NRF-2016S1A5B5A01021612).

References

  1. Kraemer WJ, Ratamess NA, French DN. Resistance training for health and performance. Curr Sports Med Rep. 2002;1:165-71. https://doi.org/10.1249/00149619-200206000-00007
  2. Yasuda T, Brechue WF, Fujita T, Sato Y, Abe T. Muscle activation during low-intensity muscle contractions with varying levels of external limb compression. J sports sci med. 2008;7:467.
  3. Takarada Y, Sato Y, Ishii N. Effects of resistance exercise combined with vascular occlusion on muscle function in athletes. Eur J Appl Physiol. 2002;86:308-14. https://doi.org/10.1007/s00421-001-0561-5
  4. Abe T, Fujita S, Nakajima T, Sakamaki M, Ozaki H, Ogasawara R. Effects of low-intensity cycle training with restricted leg blood flow on thigh muscle volume and VO2 max in young men. J sports sci med. 2010;9:452.
  5. Sudo M, Ando S, Kano Y. Repeated blood flow restriction induces muscle fiber hypertrophy. Muscle Nerve. 2017;55:274-6. https://doi.org/10.1002/mus.25415
  6. Ladlow P, Coppack RJ, Dharm-Datta S, Conway D, Sellon E, Patterson SD. Low-load resistance training with blood flow restriction improves clinical outcomes in musculoskeletal rehabilitation: A single-blind randomized controlled trial. Front Physiol. 2018;9:1269. https://doi.org/10.3389/fphys.2018.01269
  7. Yasuda T. Fukumura K, Tomaru T, Nakajima T. Thigh muscle size and vascular function after blood flow-restricted elastic band training in older women. Oncotarget. 2016;7:33595-607. https://doi.org/10.18632/oncotarget.9564
  8. Yasuda T, Loenneke JP, Thiebaud RS, Abe T. Effects of blood flow restricted low-intensity concentric or eccentric training on muscle size and strength. Plos one. 2012;7:e52843. https://doi.org/10.1371/journal.pone.0052843
  9. Hamlin MJ, Marshall HC, Hellemans J, Ainslie PN, Anglem N. Effect of intermittent hypoxic training on 20km time trial and 30s anaerobic performance. Scand J Med Sci Sports. 2010;20:651-61. https://doi.org/10.1111/j.1600-0838.2009.00946.x
  10. Sundberg C, Eiken O, Nygren A, Kaijser L. Effects of ischaemic training on local aerobic muscle performance in man. Acta Physiol Scand. 1993;148:13-9. https://doi.org/10.1111/j.1748-1716.1993.tb09526.x
  11. Ohno H, Shirato K, Sakurai T, Ogasawara J, Sumitani Y, Sato S. Effect of exercise on HIF-1 and VEGF signaling. J Phys Fit Sports Med. 2012;1:5-16. https://doi.org/10.7600/jpfsm.1.5
  12. Lundby C, Jacobs RA. Adaptations of skeletal muscle mitochondria to exercise training. Experimental physiology. 2016;101:17-22. https://doi.org/10.1113/EP085319
  13. Conceicao MS, Gaspari AF, Ramkrapes APB, Junior EMM, Bertuzzi R, Cavaglieri CR. Anaerobic metabolism induces greater total energy expenditure during exercise with blood flow restriction. PLoS One. 2018;13:e0194776. https://doi.org/10.1371/journal.pone.0194776
  14. Corvino RB, Rossiter HB, Loch T, Martins JC, Caputo F. Physiological responses to interval endurance exercise at different levels of blood flow restriction. Eur J Appl Physiol. 2017;117:39-52. https://doi.org/10.1007/s00421-016-3497-5
  15. de Oliveira MF, Caputo F, Corvino RB, Denadai BS. Shortterm low-intensity blood flow restricted interval training improves both aerobic fitness and muscle strength. Scand J Med Sci Sports. 2016;26:1017-25. https://doi.org/10.1111/sms.12540
  16. Kelly LP, Basset FA. Acute Normobaric Hypoxia Increases Post-exercise Lipid Oxidation in Healthy Males. Front Physiol. 2017;8:293. https://doi.org/10.3389/fphys.2017.00293
  17. Joyner MJ, Casey DP. Regulation of increased blood flow (hyperemia) to muscles during exercise: a hierarchy of competing physiological needs. Physiol Rev. 2015;95:549-601. https://doi.org/10.1152/physrev.00035.2013
  18. Takano H, Morita T, Iida H, Asada K, Kato M, Uno K. Hemodynamic and hormonal responses to a short-term low-intensity resistance exercise with the reduction of muscle blood flow. Eur J Appl Physiol. 2005;95:65-73. https://doi.org/10.1007/s00421-005-1389-1
  19. Hamaoka T, McCully KK, Quaresima V, Yamamoto K, Chance B. Near-infrared spectroscopy/imaging for monitoring muscle oxygenation and oxidative metabolism in healthy and diseased humans. J Biomed Opt. 2007;12:062105. https://doi.org/10.1117/1.2805437
  20. Horiuchi M, Okita K. Blood flow restricted exercise and vascular function. Int J Vasc Med. 2012;2012:543218. https://doi.org/10.1155/2012/543218
  21. Tran TK, Sailasuta N, Kreutzer U, Hurd R, Chung Y, Mole P, Kuno S, Jue T. Comparative analysis of NMR and NIRS measurements of intracellular PO2 in human skeletal muscle. Am J Physiol. 1999 Jun;276:R1682-90.
  22. Nioka S, Kime R, Sunar U, Im J, Izzetoglu M, Zhang J. A novel method to measure regional muscle blood flow continuously using NIRS kinetics information. Dyn Med. 2006;5:5. https://doi.org/10.1186/1476-5918-5-5
  23. Ganesan G, Cotter JA, Reuland W, Cerussi AE, Tromberg BJ, Galassetti P. Effect of blood flow restriction on tissue oxygenation during knee extension. Med Sci Sports Exerc. 2015;47:185-93.
  24. Suga T, Okita K, Morita N, Yokota T, Hirabayashi K, Horiuchi M. Intramuscular metabolism during low-intensity resistance exercise with blood flow restriction. J Appl Physiol. 2009;106:1119-24. https://doi.org/10.1152/japplphysiol.90368.2008
  25. Spranger MD, Krishnan AC, Levy PD, O'Leary DS, Smith SA. Blood flow restriction training and the exercise pressor reflex: a call for concern. Am J Physiol Heart Circ Physiol. 2015;309:H1440-52. https://doi.org/10.1152/ajpheart.00208.2015
  26. Mendonca GV, Vaz JR, Teixeira MS, Gracio T, Pezarat-Correia P. Metabolic cost of locomotion during treadmill walking with blood flow restriction. Clin Physiol Funct Imaging. 2014;34:308-16. https://doi.org/10.1111/cpf.12098
  27. Neto GR, Santos HH, Sousa JB, Junior AT, Araujo JP, Aniceto RR. Effects of high-intensity blood flow restriction exercise on muscle fatigue. J Hum Kinet. 2014;41:163-72. https://doi.org/10.2478/hukin-2014-0044
  28. Suga T, Okita K, Takada S, Omokawa M, Kadoguchi T, Yokota T. Effect of multiple set on intramuscular metabolic stress during low-intensity resistance exercise with blood flow restriction. Eur J Appl Physiol. 2012;112:3915-20. https://doi.org/10.1007/s00421-012-2377-x
  29. Suga T, Okita K, Morita N, Yokota T, Hirabayashi K, Horiuchi M. Dose effect on intramuscular metabolic stress during low-intensity resistance exercise with blood flow restriction. J Appl Physiol. 2010;108:1563-7. https://doi.org/10.1152/japplphysiol.00504.2009
  30. Pearson SJ, Hussain SR. A review on the mechanisms of blood-flow restriction resistance training-induced muscle hypertrophy. Sports Med. 2015;45:187-200. https://doi.org/10.1007/s40279-014-0264-9
  31. Cumming KT, Paulsen G, Wernbom M, Ugelstad I, Raastad T. Acute response and subcellular movement of HSP27, alphaB-crystallin and HSP70 in human skeletal muscle after blood-flow-restricted low-load resistance exercise. Acta Physiol (Oxf). 2014;211:634-46. https://doi.org/10.1111/apha.12305
  32. Smiles WJ, Conceicao MS, Telles GD, Chacon-Mikahil MP, Cavaglieri CR, Vechin FC, Libardi CA, Hawley JA, Camera DM. Acute low-intensity cycling with blood-flow restriction has no effect on metabolic signaling in human skeletal muscle compared to traditional exercise. Eur J Appl Physiol. 2017;117:345-58. https://doi.org/10.1007/s00421-016-3530-8
  33. Amann M, Eldridge MW, Lovering AT, Stickland MK, Pegelow DF, Dempsey JA. Arterial oxygenation influences central motor output and exercise performance via effects on peripheral locomotor muscle fatigue in humans. J Physiol. 2006;15:937-52.
  34. Miura H, McCully K, Nioka S, Chance B. Relationship between muscle architectural features and oxygenation status determined by near infrared device. Eur J Appl Physiol. 2004;91:273-8. https://doi.org/10.1007/s00421-003-0964-6