Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Histological Analysis of Reproductive System in Low-Dose Nonylphenol-treated F1 Female Mice

  • Kim, Yong-Bin (Dept. of Biotechnology, Sangmyung University) ;
  • Cheon, Yong-Pil (Division of Developmental Biology and Physiology, School of Biological Sciences and Chemistry, Sungshin University) ;
  • Choi, Donchan (Dept. of Life Science, College of Environmental Sciences, Yong-In University) ;
  • Lee, Sung-Ho (Dept. of Biotechnology, Sangmyung University)
  • Received : 2020.06.19
  • Accepted : 2020.09.07
  • Published : 2020.09.30
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Abstract

Previously, we reported adverse effects of low-dose nonylphenol (NP) exposure on the reproductive parameters of F1 female mice. In the present study we further investigated the pathohistological effect of NP exposure on the reproductive organs in F1 female mice. NP exposures were continuously conducted from parental pre-mating period until the postnatal day (PND) 33 of F1 offspring for vaginal examination. Mice were sacrificed on PND 30 and the reproductive tissue weights were measured. The initial (at PND 21) body weights of the NP-50 group animals were significantly lower than those of control group animals, and the weight deficit were recovered when the terminal (PND 33) body weights were measured. Early vaginal opening was found in NP group animals (p<0.05). Pathohistological studies revealed that NP-treated F1 animals showed prominent increase in the ovarian follicle numbers (p<0.01), and decrease in the diameter of uterine myometrium (p<0.01), and increase in the diameter of luminal epithelium (p<0.05). The present study demonstrated that the subchronic low-dose NP exposure induced early beginning of puberty and pathohistological abnormalities in ovary and uterus of F1 mice. Further studies are needed to achieve a better understanding on the action mechanism of NP in pubertal onset and to find a way to avoid a hazardous situation provoked by NP exposure.

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References

  1. Aly HA, Domenech O, Banjar ZM (2012) Effect of nonylphenol on male reproduction: Analysis of rat epididymal biochemical markers and antioxidant defense enzymes. Toxicol Appl Pharmacol 261:134-141. https://doi.org/10.1016/j.taap.2012.02.015
  2. Bontje D, Hermens J, Vermeire T, Damstra T (2012) Integrated Risk Assessment: Nonylphenol Case Study. Report No. WHO/IPCS/IRA/12/04. WHO, Geneva, Switzerland.
  3. Boukouvalas G, Antoniou K, Papalexi E, Kitraki E (2008) Post weaning high fat feeding affects rats' behavior and hypothalamic pituitary adrenal axis at the onset of puberty in a sexually dimorphic manner. Neuroscience 153:373-382. https://doi.org/10.1016/j.neuroscience.2008.02.023
  4. Cha S, Baek JW, Ji HJ, Choi JH, Kim C, Lee MY, Hwang YJ, Yang E, Lee SH, Jung HI, Cheon YP (2017) Disturbing effects of chronic low-dose 4-nonylphenol exposing on gonadal weight and reproductive outcome over one-generation. Dev Reprod 21:121-130. https://doi.org/10.12717/DR.2017.21.2.121
  5. EPA. 2009. Screening-Level Hazard Characterization: Alkylphenols Category. U.S. Environmental Protection Agency, Washington, DC.
  6. Glass AR, Dahms WT, Swerdloff RS (1979) Body fat at puberty in rats: Alteration by changes in diet. Pediatr Res 13:7-9. https://doi.org/10.1203/00006450-197901000-00002
  7. Gong Y, Wu J, Huang Y, Shen S, Han X (2009) Nonylphenol induces apoptosis in rat testicular Sertoli cells via endoplasmic reticulum stress. Toxicol Lett 186:84-95. https://doi.org/10.1016/j.toxlet.2009.01.010
  8. Guenther K, Heinke V, Thiele B, Kleist E, Prast H, Raecker T (2002) Endocrine disrupting nonylphenols are ubiquitous in food. Environ Sci Technol 36:1676-1680. https://doi.org/10.1021/es010199v
  9. Han XD, Tu ZG, Gong Y, Shen SN, Wang XY, Kang LN, Hou YY, Chen JX (2004) The toxic effects of nonylphenol on the reproductive system of male rats. Reprod Toxicol 19:215-221. https://doi.org/10.1016/j.reprotox.2004.06.014
  10. Hao C, Cheng X, Guo J, Xia H, Ma X (2013) Perinatal exposure to diethyl-hexyl-phthalate induces obesity in mice. Front Biosci (Elite Ed) 5:725-733.
  11. Kim YB, Cheon YP, Lee SH (2019) Adverse effect of nonylphenol on the reproductive system in F1 male mice: A subchronic low-dose exposure model. Dev Reprod 23:93-99. https://doi.org/10.12717/DR.2019.23.2.093
  12. Lu YY, Chen ML, Sung FC, Wang PS, Mao IF (2007) Daily intake of 4-nonylphenol in Taiwanese. Environ Int 33:903-910. https://doi.org/10.1016/j.envint.2007.04.008
  13. Lu WC, Wang AQ, Chen XL, Yang G, Lin Y, Chen YO, Hong CJ, Tian HL (2014) 90d exposure to nonylphenol has adverse effects on the spermatogenesis and sperm maturation of adult male rats. Biomed Environ Sci 27:907-911. https://doi.org/10.3967/bes2014.128
  14. Mehranjani MS, Noorafshan A, Momeni HR, Abnosi MH, Mahmoodi M, Anvari M, Hoseini SM (2009) Stereological study of the effects of vitamin E on testis structure in rats treated with para-nonylphenol. Asian J Androl 11:508-516. https://doi.org/10.1038/aja.2009.29
  15. Nagao T, Wada K, Marumo H, Yoshimura S, Ono H (2001) Reproductive effects of nonylphenol in rats after gavage administration: A two-generation study. Reprod Toxicol 15:293-315. https://doi.org/10.1016/S0890-6238(01)00123-X
  16. Noorimotlagh Z, Haghighi NJ, Ahmadimoghadam M, Rahim F (2017) An updated systematic review on the possible effect of nonylphenol on male fertility. Environ Sci Pollut Res Int 24:3298-3314. https://doi.org/10.1007/s11356-016-7960-y
  17. Rattan S, Brehm E, Gao L, Niermann S, Flaws JA (2018) Prenatal exposure to di(2-ethylhexyl) phthalate disrupts ovarian function in a transgenerational manner in female mice. Biol Reprod 98:130-145. https://doi.org/10.1093/biolre/iox154
  18. Soares A, Guieysse B, Jefferson B, Cartmell E, Lester JN (2008) Nonylphenol in the environment: A critical review on occurrence, fate, toxicity and treatment in wastewaters. Environ Int 34:1033-1049. https://doi.org/10.1016/j.envint.2008.01.004
  19. Soto AM, Justicia H, Wray JW, Sonnenschein C (1991) p-Nonyl-phenol: An estrogenic xenobiotic released from "modified" polystyrene. Environ Health Perspect 92:167-173. https://doi.org/10.1289/ehp.9192167
  20. Terasawa E, Kurian JR, Keen KL, Shiel NA, Colman RJ, Capuano SV (2012) Body weight impact on puberty: Effects of high-calorie diet on puberty onset in female rhesus monkeys. Endocrinology 153:1696-1705. https://doi.org/10.1210/en.2011-1970
  21. Uguz C, Varisli O, Agca C, Agca Y (2009) Effects of nonylphenol on motility and subcellular elements of epididymal rat sperm. Reprod Toxicol 28:542-549. https://doi.org/10.1016/j.reprotox.2009.06.007